A Molecular Genetic Analysis of Root Morphogenesis
根形态发生的分子遗传学分析
基本信息
- 批准号:10380600
- 负责人:
- 金额:$ 30.41万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-04-01 至 2024-03-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAnimalsArabidopsisBiological ProcessCell divisionCellsCoupledDaughterDevelopmentDevelopmental BiologyDiseaseEctopic ExpressionHeartImageLightLogicMapsMicroscopyModelingMolecularMolecular GeneticsMorphogenesisOrganismPathway interactionsPlant RootsPlantsPluripotent Stem CellsProcessPublic HealthRegenerative MedicineRegulationTestingTimeTissue DifferentiationWorkdevelopmental diseasegenetic analysisgenome-widehealth knowledgeinsightmathematical modelneoplastic cellnetwork modelsreal-time imagesregenerative treatmentstem cell populationstem cellstissue regeneration
项目摘要
A central question in developmental biology is, “How do cells progress from pluripotent
stem cells to fully differentiated tissues.” Stem cells divide asymmetrically to give
daughters that are launched on different trajectories. On each trajectory, cells pass
through different states as they progress toward end-stage differentiation. There are
surprisingly few cases in which this whole process has been mapped out and there are
no cases in which the regulation of the entire process is understood. Answers to this
question lie at the heart of regenerative medicine and treatment of developmental
disorders. We address this question using the root of Arabidopsis as a tractable model.
Comparing and contrasting pathways to differentiation in animals and plants allows us
to understand their underlying logic, as these evolved completely independently. Our
work has identified the core molecular network required for the division and
differentiation of one stem cell population. Mathematical modeling of this network
generated hypotheses as to how it functions. We are now experimentally testing those
hypotheses as well as imaging network dynamics in real time. We have also identified
key regulators of differentiation in this lineage. Ectopic expression of these regulators
provided insights into the stability of cell fate and the requirements for acquiring cell
fate. Our progress in characterizing the path from stem cell to differentiated tissue in the
root will allow us to address fundamental questions including, “How are formative
asymmetric cell divisions regulated?” and “What controls differentiation?” To address
these questions, we will use real time imaging with light sheet microscopy during
asymmetric cell divisions and single-cell genome-wide expression analysis during the
acquisition of cell fate. To fully understand the network motifs controlling these
processes we will reengineer them using synthetic components. Observing network
dynamics in a multicellular organism is a unique approach and has the potential to
inform basic questions regarding network function in other biological processes.
Generating synthetic network motifs coupled with mathematical modeling will provide
key insights into the logic of regulatory networks that control development as well as
into disease processes that disrupt them.
发育生物学的一个核心问题是,“细胞是如何从多能性发展而来的?
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Philip N Benfey其他文献
Cell type–specific expression profiling in plants via cell sorting of protoplasts from fluorescent reporter lines
通过荧光报告系的原生质体的细胞分选在植物中的细胞类型特异性表达分析
- DOI:
10.1038/nmeth0805-615 - 发表时间:
2005-08-01 - 期刊:
- 影响因子:32.100
- 作者:
Kenneth Birnbaum;Jee W Jung;Jean Y Wang;Georgina M Lambert;John A Hirst;David W Galbraith;Philip N Benfey - 通讯作者:
Philip N Benfey
Detecting separate time scales in genetic expression data
- DOI:
10.1186/1471-2164-11-381 - 发表时间:
2010-06-16 - 期刊:
- 影响因子:3.700
- 作者:
David A Orlando;Siobhan M Brady;Thomas MA Fink;Philip N Benfey;Sebastian E Ahnert - 通讯作者:
Sebastian E Ahnert
Philip N Benfey的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Philip N Benfey', 18)}}的其他基金
A Molecular Genetic Analysis of Root Morphogenesis
根形态发生的分子遗传学分析
- 批准号:
10598025 - 财政年份:2019
- 资助金额:
$ 30.41万 - 项目类别:
A Molecular Genetic Analysis of Root Morphogenesis
根形态发生的分子遗传学分析
- 批准号:
9902468 - 财政年份:2019
- 资助金额:
$ 30.41万 - 项目类别:
相似海外基金
The earliest exploration of land by animals: from trace fossils to numerical analyses
动物对陆地的最早探索:从痕迹化石到数值分析
- 批准号:
EP/Z000920/1 - 财政年份:2025
- 资助金额:
$ 30.41万 - 项目类别:
Fellowship
Animals and geopolitics in South Asian borderlands
南亚边境地区的动物和地缘政治
- 批准号:
FT230100276 - 财政年份:2024
- 资助金额:
$ 30.41万 - 项目类别:
ARC Future Fellowships
The function of the RNA methylome in animals
RNA甲基化组在动物中的功能
- 批准号:
MR/X024261/1 - 财政年份:2024
- 资助金额:
$ 30.41万 - 项目类别:
Fellowship
Ecological and phylogenomic insights into infectious diseases in animals
对动物传染病的生态学和系统发育学见解
- 批准号:
DE240100388 - 财政年份:2024
- 资助金额:
$ 30.41万 - 项目类别:
Discovery Early Career Researcher Award
RUI:OSIB:The effects of high disease risk on uninfected animals
RUI:OSIB:高疾病风险对未感染动物的影响
- 批准号:
2232190 - 财政年份:2023
- 资助金额:
$ 30.41万 - 项目类别:
Continuing Grant
RUI: Unilateral Lasing in Underwater Animals
RUI:水下动物的单侧激光攻击
- 批准号:
2337595 - 财政年份:2023
- 资助金额:
$ 30.41万 - 项目类别:
Continuing Grant
A method for identifying taxonomy of plants and animals in metagenomic samples
一种识别宏基因组样本中植物和动物分类的方法
- 批准号:
23K17514 - 财政年份:2023
- 资助金额:
$ 30.41万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Analysis of thermoregulatory mechanisms by the CNS using model animals of female-dominant infectious hypothermia
使用雌性传染性低体温模型动物分析中枢神经系统的体温调节机制
- 批准号:
23KK0126 - 财政年份:2023
- 资助金额:
$ 30.41万 - 项目类别:
Fund for the Promotion of Joint International Research (International Collaborative Research)
Using novel modelling approaches to investigate the evolution of symmetry in early animals.
使用新颖的建模方法来研究早期动物的对称性进化。
- 批准号:
2842926 - 财政年份:2023
- 资助金额:
$ 30.41万 - 项目类别:
Studentship
Study of human late fetal lung tissue and 3D in vitro organoids to replace and reduce animals in lung developmental research
研究人类晚期胎儿肺组织和 3D 体外类器官在肺发育研究中替代和减少动物
- 批准号:
NC/X001644/1 - 财政年份:2023
- 资助金额:
$ 30.41万 - 项目类别:
Training Grant