DanFreez: Zebrafish Genetically-Optimized for Cryogenic Storage of Embryos

总结： 与C.由于斑马鱼和小鼠的遗传多样性，市场上缺乏冷藏斑马鱼品系的方法。我们建议 使用环境和基因C因子以使能存活的胚胎能够在冷冻或接近冷冻的条件下冷藏。 在目标1中，我们将探索使用瞬时蛋白表达来产生与脱水生物状态相关的蛋白。 在目标2中，我们探索了诱导与鱼类滞育有关的基因作为促进冷藏的机制。 生存在目标3中，我们将研究基因c和环境因素的组合， 斑马鱼胚胎从冷藏库中取出。实现这些目标中的任何一个，导致从寒冷中获得高活力产量 然后，在第二阶段中，可以将储存物开发为基因整合系，然后可以将其广泛商业化， 产品或服务，以帮助研究人员在他们的生物银行电子商务与他们的斑马鱼线。

Summary: Unlike C. elegans and mice, methods for cold storage of zebrafish lines are lacking in the marketplace. We propose the use of environmental and gene c factors to enable cold storage of viable embryos at frozen or near freezing condi ons. In Aim 1 we will explore the use of transient protein expression to produce proteins associated with anhydrobio c status. In Aim 2 we explore the induc on of genes involved in diapause in killifish as a mechanism to promote cold-storage survival. In Aim 3, we will look at combina ons of gene c and environmental factors that promote reanima on of zebrafish embryos from cold storage. Achievement of any one of these aims that result in high viability yields from cold storage can then be developed in Phase II as a gene cally integrated line, which can then be broadly commercialized as a product or service to aid researchers in their biobanking efforts with their zebrafish lines.