Structure, Mechanism and Interactions of Type IA Topoisomerases
Structure, Mechanism and Interactions of Type IA Topoisomerases
批准号:
10389425
负责人:
Yuk-Ching Tse-Dinh
金额:
$6.03万
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
未结题
起止时间:
2021-02-01 至 2026-01-31
关键词:
ComplexCrystallizationDNAEquipmentEscherichia coliFundingGrantHumanLigandsMolecular ConformationMycobacterium tuberculosisNational Institute of General Medical SciencesProteinsRNARecombinantsRequest for ApplicationsResearchStructureSystemTimeTopoisomeraseTopoisomerase IIIType I DNA TopoisomerasesUnited States National Institutes of HealthX-Ray Crystallographybiophysical analysisfast protein liquid chromatographyinhibitor/antagonistmilligrammutantprotein protein interactionprotein purificationrepairedsingle molecule
中文摘要
PI: Yuk-Ching Tse-Dinh
英文摘要
Project Summary PI: Yuk-Ching Tse-Dinh
This equipment supplement application requests funds to purchase an AKTA FPLC protein
purification system for the large scale purification of recombinant topoisomerase proteins that
are needed for obtaining new structures of topoisomerases by X-ray crystallography, and
biophysical studies to elucidate topoisomerase complex conformations, protein-protein
interactions and ligand interaction dynamics under the aims of the PI’s NIGMS active grants
R01-GM054226 and R35-GM139817. The AKTA FPLC protein purification system currently
available in the PI’s lab requires frequent repairs and needs to be replaced to avoid extensive
down times. The replacement of the AKTA FPLC purification system would allow us to produce
quickly and efficiently without bottlenecks milligram quantities of wild-type and mutant forms of
Escherichia coli and Mycobacterium tuberculosis topoisomerase I, E. coli topoisomerase III, and
human topoisomerase IIIB for structural and biophysical studies of their action on both DNA and
RNA substrates, as well as inhibitors. The proteins need to be produced in high purity for
studies in our lab at FIU, and are also needed by our collaborators at Argonne National Lab and
NIH for crystallization screens and single-molecule studies.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Structure, Mechanism and Interactions of Type IA Topoisomerases
-
批准号:10093404
-
项目类别:
-
资助金额:$20.92万
-
财政年份:2021
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
Structure, Mechanism and Interactions of Type IA Topoisomerases
-
批准号:10569676
-
项目类别:
-
资助金额:$34.15万
-
财政年份:2021
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
HTS assay development targeting Yersinia pestis topoisomerase I
-
批准号:8234706
-
项目类别:
-
资助金额:$3.98万
-
财政年份:2010
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
HTS assay development targeting Yersinia pestis topoisomerase I
-
批准号:7991064
-
项目类别:
-
资助金额:$15.9万
-
财政年份:2010
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
Bacterial cell killing by topoisomerase I mediated DNA lesion
-
批准号:8070106
-
项目类别:
-
资助金额:$3.42万
-
财政年份:2010
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
Bacterial cell killing by topoisomerase I mediated DNA lesion
-
批准号:7756650
-
项目类别:
-
资助金额:$36.78万
-
财政年份:2006
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
Bacterial cell killing by topoisomerase I mediated DNA lesion
-
批准号:7169238
-
项目类别:
-
资助金额:$37.6万
-
财政年份:2006
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
Bacterial cell killing by topoisomerase I mediated DNA lesion
-
批准号:8186092
-
项目类别:
-
资助金额:$40.25万
-
财政年份:2006
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
Bacterial cell killing topoisomerase I--DNA lesion
-
批准号:7083065
-
项目类别:
-
资助金额:$38.44万
-
财政年份:2006
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
Bacterial cell killing by topoisomerase I mediated DNA lesion
-
批准号:7333269
-
项目类别:
-
资助金额:$37.15万
-
财政年份:2006
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
Bacterial cell killing by topoisomerase I mediated DNA lesion
-
批准号:8324194
-
项目类别:
-
资助金额:$37.86万
-
财政年份:2006
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
Bacterial cell killing by topoisomerase I mediated DNA lesion
-
批准号:8522124
-
项目类别:
-
资助金额:$33.68万
-
财政年份:2006
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
Bacterial cell killing by topoisomerase I mediated DNA lesion
-
批准号:7541787
-
项目类别:
-
资助金额:$37.15万
-
财政年份:2006
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
Development of a HTS system:topoisomerase targets (RMI)
-
批准号:6879445
-
项目类别:
-
资助金额:$7.8万
-
财政年份:2004
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
CONTROL OF DNA TOPOLOGY
-
批准号:6636188
-
项目类别:
-
资助金额:$27.39万
-
财政年份:1996
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
Control of DNA Topology
-
批准号:8403014
-
项目类别:
-
资助金额:$22.85万
-
财政年份:1996
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
Control of DNA Topology
-
批准号:7207966
-
项目类别:
-
资助金额:$29.15万
-
财政年份:1996
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
Control of DNA Topology
-
批准号:7579890
-
项目类别:
-
资助金额:$29.33万
-
财政年份:1996
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
CONTROL OF DNA TOPOLOGY
-
批准号:2392285
-
项目类别:
-
资助金额:$19.88万
-
财政年份:1996
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
CONTROL OF DNA TOPOLOGY
-
批准号:2193617
-
项目类别:
-
资助金额:$19.13万
-
财政年份:1996
-
负责人:Yuk-Ching Tse-Dinh
-
依托单位:
海外基金