Development of Multidimensional, Linear and Differential Ion Mobility MS Separations for Middle-Down Proteoforms

项目摘要PI：Francisco Fernandez-Lima 该设备补充申请请求资金来购买LEAP HDX基础系统， 和相应的HDExminer数据分析软件来支持仪器方面的进步 串联离子迁移率-质谱仪(IMS/IMS-MS/MS)，目标是提供所需的 补充为以下方面制定的中下战略和自上而下战略的结构信息 DNA结合蛋白的结构表征及配基相互作用动力学 PI的NIGMS主动赠款R01-GM134247的目标。此设备补充请求 还将简化解决方案HDX技术的应用， FIU几个主动资助的NIGMS调查人员的自下而下和自上而下的IMS-MS战略 通过先进的质谱学设备，由PI指导。

Project Summary PI: Francisco Fernandez-Lima This equipment supplement application requests funds to purchase a LEAP HDX Base System, and corresponding HDExaminer Data Analysis Software to support instrumental advances in tandem ion mobility – mass spectrometry (IMS/IMS-MS/MS) with the goal of providing needed complementary structural information to the middle-down and top-down strategies developed for the structural characterization of DNA binding proteins and ligand interaction dynamics under the aims of the PI’s NIGMS active grant R01-GM134247. This equipment supplement request will also streamline the application of solution HDX technology in tandem with unique middle- down and top-down IMS- MS strategies for several active-funded NIGMS investigators at FIU via the Advance Mass Spectrometry Facility, directed by the PI.