Development of Multidimensional, Linear and Differential Ion Mobility MS Separations for Middle-Down Proteoforms

中下蛋白质组多维、线性和微分离子淌度 MS 分离的开发

• 批准号: 10389482
• 负责人: Francisco Fernandez-Lima
• 金额: $ 12.65万
• 依托单位: FLORIDA INTERNATIONAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-20 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10389482
• 关键词: Amino Acid Sequence; Area; Atmospheric Pressure; Biological; Cells; Communities; Computer software; Custom; DNA-Binding Proteins; Data Analyses; Development; Devices; Dissociation; Electrons; Epigenetic Process; Epithelial Cells; Equipment; Funding; Geometry; Germ-Free; Goals; Grant; Histone Code; Histones; Isomerism; Ligands; Liquid Chromatography; Mass Spectrum Analysis; Methodology; Methods; Mus; National Institute of General Medical Sciences; Pattern; Positioning Attribute; Post-Translational Protein Processing; Property; Protein Isoforms; Proteomics; Request for Applications; Research Personnel; Resolution; Science; Speed; Structure; System; Technology; Testing; Variant; base; biological systems; epigenetic regulation; gut bacteria; innovation; instrumentation; ion mobility; ionization; liquid chromatography mass spectrometry; next generation; pressure; success; tandem mass spectrometry

Project Summary PI: Francisco Fernandez-Lima This equipment supplement application requests funds to purchase a LEAP HDX Base System, and corresponding HDExaminer Data Analysis Software to support instrumental advances in tandem ion mobility – mass spectrometry (IMS/IMS-MS/MS) with the goal of providing needed complementary structural information to the middle-down and top-down strategies developed for the structural characterization of DNA binding proteins and ligand interaction dynamics under the aims of the PI’s NIGMS active grant R01-GM134247. This equipment supplement request will also streamline the application of solution HDX technology in tandem with unique middle- down and top-down IMS- MS strategies for several active-funded NIGMS investigators at FIU via the Advance Mass Spectrometry Facility, directed by the PI.

项目总结PI：弗朗西斯科费尔南德斯-利马 该设备补充申请要求资金购买LEAP HDX基础系统， 和相应的HDExaminer数据分析软件，以支持仪器的进步， 串联离子迁移-质谱（IMS/IMS-MS/MS）的目标是提供所需的 补充结构信息的中间和自上而下的战略制定， DNA结合蛋白的结构表征和配体相互作用动力学 PI的NIGMS主动资助R 01-GM 134247的目的。本次设备补充申请 还将简化解决方案HDX技术的应用， 为金融情报室几名积极资助的国家情报、地理和监测系统调查人员制定了自下而上和自上而下的国际监测系统-监测系统战略 通过高级质谱设备，由PI指导