Molecular mechanisms that regulate polarity and spindle orientation in animals

项目摘要 要求提供资金用于购买Akta Pure 25 Protein Purifi阳离子系统 纯化检测PAR复合体生化活性所需的试剂，a 母公司拨款的关键方面，R35GM127092。重构和审问PAR情结 为了了解极性被调节的机制，高纯度的试剂 必填项。这是具有挑战性的，原因有两个。首先，PAR情结本身就是diffi邪教获得的 它是从瞬时转基因的培养细胞中获得的。第二，我们的工作需要 各种修饰形式的PAR复杂蛋白(例如截断)，该仪器将允许 美国以高分辨率将它们与杂质分离。我们预计，额外的 此工具提供的功能将对以下方面产生显著的fi影响和持续的积极影响 我们的研究计划增加了关键试剂的可靠性和重复性。

Project abstract Funding is requested for the purchase of an AKTA pure 25 protein purification system for purifying the reagents necessary for examining the biochemical activity of the Par complex, a key aspect of the parent grant, R35GM127092. To reconstitute and interrogate Par complex proteins to understand the mechanism by which polarity is regulated, highly pure reagents are required. This is challenging for two reasons. First, the Par complex itself is difficult to obtain as it is obtained from transiently transfected cultured cells. Second, our work requires making various modified forms of Par complex proteins (e.g. truncations) and this instrument will allow us to separate them from impurities with high resolution. We expect that the additional capabilities provided by this instrument will have a significant and sustained positive impact on our research program by increasing the reliability and reproducibility of key reagents.