Molecular mechanisms that regulate polarity and spindle orientation in animals

项目摘要

Project abstract Funding is requested for the purchase of an AKTA pure 25 protein purification system for purifying the reagents necessary for examining the biochemical activity of the Par complex, a key aspect of the parent grant, R35GM127092. To reconstitute and interrogate Par complex proteins to understand the mechanism by which polarity is regulated, highly pure reagents are required. This is challenging for two reasons. First, the Par complex itself is difficult to obtain as it is obtained from transiently transfected cultured cells. Second, our work requires making various modified forms of Par complex proteins (e.g. truncations) and this instrument will allow us to separate them from impurities with high resolution. We expect that the additional capabilities provided by this instrument will have a significant and sustained positive impact on our research program by increasing the reliability and reproducibility of key reagents.