Molecular mechanisms that regulate polarity and spindle orientation in animals

项目摘要 申请资金用于购买AKTA pure 25蛋白纯化系统， 纯化检测Par复合物的生化活性所必需的试剂， 父母补助金的关键方面，R35GM127092。重建和询问Par复合体 为了了解蛋白质的极性调节机制， 必需的.这是具有挑战性的，原因有二。首先，Par复合体本身很难获得， 它从瞬时转染的培养细胞中获得。第二，我们的工作要求 Par复合蛋白的各种修饰艾德形式（例如截短），该仪器将允许 我们将它们与杂质分离，具有高分辨率。我们预计， 该工具提供的能力将对以下方面产生重大和持续的积极影响： 通过提高关键试剂的可靠性和重现性，我们的研究计划。

Project abstract Funding is requested for the purchase of an AKTA pure 25 protein purification system for purifying the reagents necessary for examining the biochemical activity of the Par complex, a key aspect of the parent grant, R35GM127092. To reconstitute and interrogate Par complex proteins to understand the mechanism by which polarity is regulated, highly pure reagents are required. This is challenging for two reasons. First, the Par complex itself is difficult to obtain as it is obtained from transiently transfected cultured cells. Second, our work requires making various modified forms of Par complex proteins (e.g. truncations) and this instrument will allow us to separate them from impurities with high resolution. We expect that the additional capabilities provided by this instrument will have a significant and sustained positive impact on our research program by increasing the reliability and reproducibility of key reagents.