Administrative Supplement: Dynamics and evolution of synthetic and natural gene regulatory networks

行政补充：合成和天然基因调控网络的动态和进化

• 批准号: 10388886
• 负责人: Gabor Balazsi
• 金额: $ 23.29万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10388886
• 关键词: Address; Administrative Supplement; Affect; Behavior; Biology; Biopsy; Biotechnology; Cell Separation; Cells; Clustered Regularly Interspaced Short Palindromic Repeats; Complex; Computer Models; Coupled; Engineering; Evolution; Gene Expression Profile; Genes; Genetic; Genomics; Learning; Liquid substance; Malignant Neoplasms; Microbial Drug Resistance; Modification; Molecular Profiling; National Institute of General Medical Sciences; Population; Proteomics; Regulator Genes; Signal Transduction; Synthetic Genes; System; Time; base; equipment acquisition; genetic payload; interest; parent grant; parent project; prevent; recombinase; transcriptomics

Project Summary: Administrative Supplement to NIGMS MIRA R35GM122561 Parent project title: Dynamics and evolution of synthetic and natural gene regulatory networks This Administrative Supplement is based on NOT-GM-21-030, “Notice of Special Interest (NOSI): Administrative Supplements for Equipment Purchases for NIGMS R01, R35, and R37 Awardees”. It is a request for partial support of a FluidFM Bio system by Cytosurge AG, a Swiss Biotech company. The FluidFM system has unique capabilities for repeated, single live cell-specific, direct intra- cytoplasmic or intra-nuclear delivery and extraction of fluids, as well as nondestructive single cell manipulation. These capabilities will be transformative in promoting progress with the parent grant, which aims to learn how gene network dynamics and stochasticity affect single cells and thereby cell populations. To achieve this, we proposed using synthetic gene networks to generate specific gene expression patterns in space and time that serve as signals for natural gene networks, studying the subsequent effects on cell population behavior and evolution by computational modeling and experimental evolution. The FluidFM system will accelerate the stable genomic insertion of synthetic gene circuits by coupled intranuclear delivery of CRISPR, recombinase and genetic payloads into single cells, by cell isolation for clonal outgrowth, and by repeated “biopsies” taken from engineered single living cells to study how their transcriptomic and proteomic networks respond to perturbations by synthetic gene circuits. Overall, the FluidFM system will provide unique capabilities to illuminate how complex networks enable control across scales of space and time in biology, from molecules to cells. Addressing these questions will teach us how to control adapting cell populations, which is relevant for understanding, predicting and possibly preventing cancer and microbial drug resistance.

项目摘要：NIGMS MIRA R35GM122561行政补充