Project 1: Immune Memory
项目一:免疫记忆
基本信息
- 批准号:10394365
- 负责人:
- 金额:$ 251.68万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-06-02 至 2023-04-30
- 项目状态:已结题
- 来源:
- 关键词:2019-nCoVAcuteAdultAntibodiesAntibody ResponseB-LymphocytesBindingBiological AssayCellsClinicConvalescenceCoronavirusDevelopmentDiagnosticDiseaseElderlyEmerging Communicable DiseasesGene Expression ProfileGoalsHealth PersonnelHumanHumoral ImmunitiesImmune responseImmunologic MemoryInfectionPeripheral Blood Mononuclear CellProtocols documentationSARS-CoV-2 antibodySARS-CoV-2 infectionSamplingSerologySerology testSerumSourceTestingTherapeuticUniversity HospitalsUrban HospitalsVaccinesViralVirusVirus Diseasesacute infectionadaptive immune responseagedbiosafety level 3 facilityexpression cloninghuman monoclonal antibodiesinsightinstrumentresponseseroconversionserosurveillancetoolvaccine-induced immunity
项目摘要
The over-arching goal of this proposal is to analyze SARS-CoV-2 antibody seroconversion rates in health
care workers at the Emory University Hospital over the next year and to study adaptive immune responses
to SARS-CoV-2 infection during acute infection and at convalescence. These studies will draw on three
main sources of samples, either through an emerging infectious disease protocol at the Emory Vaccine
Center Hope Clinic, through a sero-surveillance study initiated at Emory, and previously collected PBMC
and serum samples from adult or elderly donors by Dr. Jorg Goronzy, respectively. We will develop and
employ a combination of antibody binding assays and viral neutralization assays to test the quantity and
quality of the infection-induced antibody responses. This information will also be used to identify optimal
donors for single-cell antibody expression cloning, as we have previously described9-13. A recently
purchased 10x instrument in our BSL3 facility at the Emory Vaccine Center will allow for a detailed analysis
of the transcriptional profiles of innate and adaptive immune responses during acute SARS-CoV-2
infection. The proposed studies will provide key insight into the dynamics of this devastating disease
among health care workers at a major metropolitan hospital in Atlanta. These efforts will also generate key
serological tools, provide an understanding of the humoral immune response to SARS-CoV-2 infection,
and generate human monoclonal antibodies, with both diagnostic and therapeutic potential.
该提案的首要目标是分析健康人群中的 SARS-CoV-2 抗体血清转化率
埃默里大学医院的护理人员明年将研究适应性免疫反应
急性感染期间和恢复期的 SARS-CoV-2 感染。这些研究将借鉴三个
样本的主要来源,要么通过埃默里疫苗局的新发传染病方案
Center Hope Clinic,通过埃默里大学发起的一项血清监测研究,之前收集了 PBMC
以及 Jorg Goronzy 博士分别采集的成人或老年捐献者的血清样本。我们将开发和
采用抗体结合测定和病毒中和测定的组合来测试数量和
感染引起的抗体反应的质量。该信息还将用于确定最佳
正如我们之前所描述的9-13,单细胞抗体表达克隆的供体。最近一个
在埃默里疫苗中心的 BSL3 设施中购买的 10 倍仪器将可以进行详细分析
急性 SARS-CoV-2 期间先天性和适应性免疫反应的转录谱
感染。拟议的研究将为了解这种毁灭性疾病的动态提供重要见解
亚特兰大一家大都会医院的医护人员。这些努力也将产生关键
血清学工具,提供对 SARS-CoV-2 感染的体液免疫反应的了解,
并产生具有诊断和治疗潜力的人类单克隆抗体。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Rafi Ahmed其他文献
Rafi Ahmed的其他文献
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{{ truncateString('Rafi Ahmed', 18)}}的其他基金
System Biological Analyses of Innate and Adaptive Responses to Vaccination
对疫苗接种的先天和适应性反应的系统生物学分析
- 批准号:
10345981 - 财政年份:2021
- 资助金额:
$ 251.68万 - 项目类别:
System Biological Analyses of Innate and Adaptive Responses to Vaccination
对疫苗接种的先天和适应性反应的系统生物学分析
- 批准号:
10375723 - 财政年份:2021
- 资助金额:
$ 251.68万 - 项目类别:
System Biological Analyses of Adaptive Responses to vaccination
疫苗接种适应性反应的系统生物学分析
- 批准号:
10201503 - 财政年份:2020
- 资助金额:
$ 251.68万 - 项目类别:
Exploiting the Mechanobiology of PD-1 for Cancer Immunotherapy
利用 PD-1 的力学生物学进行癌症免疫治疗
- 批准号:
10174887 - 财政年份:2020
- 资助金额:
$ 251.68万 - 项目类别:
System Biological Analyses of Innate and Adaptive Responses to Vaccination
对疫苗接种的先天和适应性反应的系统生物学分析
- 批准号:
10056675 - 财政年份:2020
- 资助金额:
$ 251.68万 - 项目类别:
Exploiting the Mechanobiology of PD-1 for Cancer Immunotherapy
利用 PD-1 的力学生物学进行癌症免疫治疗
- 批准号:
10408747 - 财政年份:2020
- 资助金额:
$ 251.68万 - 项目类别:
Exploiting the Mechanobiology of PD-1 for Cancer Immunotherapy
利用 PD-1 的力学生物学进行癌症免疫治疗
- 批准号:
10524207 - 财政年份:2020
- 资助金额:
$ 251.68万 - 项目类别:
System Biological Analyses of Innate and Adaptive Responses to Vaccination
对疫苗接种的先天和适应性反应的系统生物学分析
- 批准号:
10201491 - 财政年份:2020
- 资助金额:
$ 251.68万 - 项目类别:
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