EMRSHN: Exploring the Modulatory Role of Sec Hormones Along the Neuromechanical Axis in Females

项目摘要 在我们目前的R01的基础上，我们建议表征全身激素的影响 浓度对骨骼肌基本特性的影响。最初，年轻和其他方面健康 将招募女性外科患者来确定肌肉之间的关联映射 骨骼纤维组成和雌二醇浓度与组织学及蛋白质/基因表达的关系 肌肉活组织检查。下一步，将招募年轻健康的女性受试者来确定潜力 在月经周期期间，肌肉纤维组成在有限的时间窗口内变化，使用 最先进的7特斯拉扫描仪进行磁共振波谱分析。最后，年轻和 将招募健康的女性受试者来确定内分泌的潜在影响 运动过程中监测循环肌群和肌肉活动对肌肉可塑性的影响。 如果成功完成，这些目标将揭示出受试者的 内分泌状态和基础肌肉表型的急性变化及其相关变化 在神经肌肉控制方面。综上所述，这些目标有可能阐明 肌肉骨骼损伤中可能导致性别差异的潜在生物学机制 并指导今后伤害预防策略的研究。

Project Summary Building off of our current R01, we propose to characterize the effect of systemic hormone concentrations on basic skeletal muscle characteristics. Initially, young and otherwise healthy female surgical patients will be recruited to determine an associative mapping between muscle fiber composition and E2 concentration using histology and protein/gene expression of skeletal muscle biopsies. Next, young and healthy female subjects would be recruited to identify potential muscle fiber composition changes over a limited time window during the menstrual cycle, using a state-of-the-art 7 Tesla scanner to perform magnetic resonance spectroscopy. Finally, young and healthy female subjects would be recruited to identify the potential effect of the endocrinological state on muscle plasticity by monitoring circulating myomiRs and muscle activity during exercise. If successfully completed, these aims would reveal a relationship between a subject’s endocrinological state and acute changes in basic muscle phenotype and the associated changes in neuromuscular control. Taken together these aims have the potential to elucidate the underlying biological mechanisms that may drive the sex-disparity seen in musculoskeletal injury and to guide future research in injury prevention strategies.