Center for Synthetic Regulatory Genomics

修改项目摘要/摘要部分 合成调控基因组学中心（SyRGe）的任务是开发和应用革命性技术，对广泛的基因位点进行戏剧性的，协调的改变，这将使调控序列的功能得到广泛的研究，并促进生物技术，个性化医学和基因治疗的翻译应用。具体来说，我们将（i）设计和合成超过1000个> 100 kbp的构建体，以探测在一组精心选择的基因上的调控功能;（ii）开发一个管道，用于在基因组范围内的任何所需位点进行单拷贝整合;（iii）调查文库对基因表达、染色质特征和核结构的影响;以及（iv）开发全局计算模型以预测序列变异对基因表达的调节作用。该中心将大大取代目前和预测的技术，用于操纵和评估调控基因组功能。该中心还结合了一个独特的和非常成功的推广计划，使来自不同背景的本科生在基因组组装中发挥关键作用，以及一个新的研究员计划，使来自其他领域的研究人员和学生接触变革性的新技术，并促进其在更大的人类遗传学和基因组学社区的颁布。

Modified Project Summary/Abstract Section The Center for Synthetic Regulatory Genomics (SyRGe) is tasked with development and application of revolutionary technology for making dramatic, coordinated changes to extensive gene loci, which will enable broad investigation of the function of regulatory sequences and foster translational applications to biotechnology, personalized medicine and gene therapy. Specifically, we will (i) design and synthesize over 1000 >100kbp constructs probing regulatory function at a carefully selected set of genes; (ii) develop a pipeline for their single-copy integration at any desired site genome-wide; (iii) survey libraries for effects on gene expression, chromatin features, and nuclear architecture; and (iv) develop global computational models to predict regulatory effects of sequence variation on gene expression. The Center will dramatically supersede present and predicted technologies for manipulation and assessment of regulatory genome function. This Center also incorporates a unique and highly successful outreach program whereby undergraduates from diverse backgrounds play a crucial role in genome assembly, as well as a new Fellows program to expose researchers and students from other fields to transformative new technology and facilitate its promulgation throughout the larger human genetics and genomics communities.