Core B - Retrotransposon Genomics, Technology and Analysis Core

核心摘要 核心B将提供尖端工具来测量后转位 衰老范式背景下的多细胞真核生物 阿尔茨海默氏症及相关疾病。除了提供适当的 现有的所有参与者的化验和鼠标模型，核心也将 开发改进的逆转录转位记者和基因组学工具，以使 在各种实验中检测和定位逆转位事件 正在研究的系统。核心也将提供“驱动系统”，以导致 这些元件在细胞系统和动物中的高水平表达。最后， 核心将提供尖端的排序和计算工具来分析 逆转录转座本身和LINE-1表达的异常形式 在老化的细胞和生物体中观察到的逆转录。

Core Summary Core B will provide cutting edge tools to measure retrotransposition in multicellular eukaryotes in the context of aging paradigms with a special focus on Alzheimer’s disease and related disorders. In addition to providing appropriate existing assays and mouse models to all of the participants, the core will also develop improved retrotransposition reporters and genomics tools to enable the detection and mapping of retrotransposition events in the various experimental systems under study. The Core will also provide “Driver systems” that lead to high level expression of these elements in both cell systems and animals. Finally, the core will provide cutting edge sequencing and computational tools to analyze both retrotransposition itself and the aberrant forms of LINE-1 expression and reverse transcription observed in aging cells and organisms.