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Core B - Retrotransposon Genomics, Technology and Analysis Core

核心 B - 逆转录转座子基因组学、技术和分析核心

基本信息

批准号：
10581511
负责人：
Jef D BOEKE
金额：
$ 49.74万
依托单位：
BROWN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-01 至 2026-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10581511
关键词：
Affinity Chromatography Aging Alzheimer&apos s Disease Alzheimer&apos s disease related dementia Animal Model Animals Area Biological Biological Assay Biology Cell Aging Cells ChIP-seq Clinical Collaborations Complementary DNA Complex Computer Analysis Computing Methodologies Cytoplasm DNA Data Data Set Dementia Detection Drosophila genus Elements Engineering Environment Eukaryota Event Genes Genomics Germ Lines Goals Grant Hybrids Imaging technology Individual Interphase Cell L1 Elements Life Cycle Stages Maps Mass Spectrum Analysis Measurement Measures Methods Modeling Monitor Mus Nerve Degeneration Neurodegenerative Disorders Organism Participant Property Proteins Proteomics RNA immunoprecipitation sequencing Reporter Reporting Retrotransposition Retrotransposon Reverse Transcription Role Sampling Sequence Analysis Stimulator of Interferon Genes System Technology Tissues Transcript Transcriptional Regulation Translations Visualization Work aging brain cell type computerized tools demethylation design fascinate gene product genomic locus genomic tools imaging modality improved in vivo insight interest mouse model novel permissiveness postmitotic promoter response single cell sequencing single molecule single-cell RNA sequencing technological innovation technology validation tool transcriptome sequencing transcriptomics

项目摘要

Core Summary Core B will provide cutting edge tools to measure retrotransposition in multicellular eukaryotes in the context of aging paradigms with a special focus on Alzheimer’s disease and related disorders. In addition to providing appropriate existing assays and mouse models to all of the participants, the core will also develop improved retrotransposition reporters and genomics tools to enable the detection and mapping of retrotransposition events in the various experimental systems under study. The Core will also provide “Driver systems” that lead to high level expression of these elements in both cell systems and animals. Finally, the core will provide cutting edge sequencing and computational tools to analyze both retrotransposition itself and the aberrant forms of LINE-1 expression and reverse transcription observed in aging cells and organisms.

核心摘要核心B将提供尖端工具，以测量多细胞真核生物在衰老的背景下，特别关注阿尔茨海默病及相关疾病。除了提供适当的现有的分析和小鼠模型的所有参与者，核心还将开发改进的反转录转座报告和基因组学工具，在各种实验中检测和定位反转录转座事件研究中的系统。核心还将提供“驱动程序系统”，这些元件在细胞系统和动物中的高水平表达。最后，核心将提供最先进的测序和计算工具，逆转录转座本身和LINE-1表达的异常形式，在衰老细胞和生物体中观察到的逆转录。