Mass Spectrometry Core

质谱核心

• 批准号: 10392935
• 负责人: Sophie Alvarez
• 金额: $ 5.17万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-03-16 至 2023-07-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10392935
• 关键词: Address; Affect; Affinity Chromatography; Applications Grants; Biological Assay; Biotechnology; Blood; Breast Cancer cell line; Cell Line; Chemistry; Complex; Computer software; DNA-protein crosslink; Data; Development; Drug Delivery Systems; Drug Targeting; Encapsulated; Ensure; Equipment; Experimental Designs; Genetic; Goals; Human; Immunoprecipitation; Infrastructure; Knock-out; Label; Link; Liquid Chromatography; Mantle Cell Lymphoma; Mass Spectrum Analysis; Mediating; Methods; Molecular Target; Monitor; Nanostructures; Nebraska; Pharmaceutical Preparations; Pharmacotherapy; Phenotype; Phosphopeptides; Phosphorylation; Phosphorylation Site; Plasma; Post-Translational Protein Processing; Preparation; Procedures; Process; Proteins; Proteome; Proteomics; Reagent; Regulation; Regulatory Pathway; Research; Research Personnel; Running; STAT3 gene; Sampling; Services; Signal Pathway; Signal Transduction; Site; Spectrometry, Mass, Electrospray Ionization; Stimulus; Students; TRAF6 gene; Technology; Therapeutic Intervention; U937 Cells; Ubiquitination; Universities; Validation; Wood material; analytical tool; base; bioinformatics pipeline; biomarker identification; cancer therapy; computing resources; cost effective; experience; experimental study; instrumentation; metabolomics; mutant; peptide amphiphiles; protein protein interaction; repaired; response; screening; skills; student training; tandem mass spectrometry; targeted treatment; therapeutic target; titanium dioxide; tool

Project Summary/Abstract: Mass Spectrometry Core (Core 2) The Mass Spectrometry Core (MSC) will collaborate with and support the project leaders within the Nebraska Center for Molecular Target Discovery and Development in the identification and validation of new protein targets for therapeutic drug treatment of cancers. The Core will utilize the newly established infrastructure and analytical equipment from the Proteomics and Metabolomics Facility (PMF) of the Center for Biotechnology at the University of Nebraska – Lincoln, which receives support from the Nebraska Research Initiative (NRI). The new MSC core will be immediately operational to accept projects from the Project Leaders of the new Center. The objective for the requested support for the MSC is to afford comprehensive services to the Project Leaders and to introduce new analytical capabilities using the instrumentation and computing resources available. The MSC will strive to remain state-of-the art, responding to and addressing the specific needs of the project aims described by each Project Leader in the grant proposal. The Center will use the expertise and skills of the leader Dr. Alvarez and Co-Investigator Dr. Naldrett in proteomics and mass spectrometry. The major goals of the MSC are:  to assist the Project Leaders with the experimental design and sample preparation required for the mass spectrometry-based experiments described in the grant proposal.  to process and run samples requiring protein identification and/or quantification using mass spectrometry and other analytical tools.  to optimize platforms for new PTMomics (post-translational modifications) capabilities using mass spectrometry.  to analyze the data acquired and assist with the interpretation of the results using the bioinformatic pipelines available in the core.  to educate the Project Leaders and their students on best practice.  to offer training of students involved in the projects on use of analytical equipment and analysis of mass spectrometry data.

项目概要/摘要：质谱核心（核心2） 质谱核心（MSC）将与内布拉斯加州的项目负责人合作并提供支持。 新蛋白质鉴定和验证的分子靶标发现和开发中心 用于癌症的治疗药物治疗的靶点。核心将利用新建立的基础设施， 来自生物技术中心蛋白质组学和代谢组学设施（PMF）的分析设备， 内布拉斯加大学-林肯分校，它得到内布拉斯加研究计划（NRI）的支持。的 新的MSC核心将立即投入使用，接受新中心项目负责人的项目。 要求为海安会提供支助的目的是向项目领导人提供全面服务 并使用可用的仪器和计算资源引入新的分析能力。的 海安会将努力保持最先进的技术水平，响应和解决项目目标的具体需求。 由每个项目负责人在赠款建议书中描述。该中心将利用其专业知识和技能， Alvarez博士和蛋白质组学和质谱学的共同研究员Naldrett博士。 海安会的主要目标是： 协助项目负责人进行所需的实验设计和样品制备， 资助提案中描述的基于质谱的实验。 用于处理和运行需要蛋白质鉴定和/或使用质谱定量的样品 光谱分析和其他分析工具。 优化平台以实现新的PTMomics（翻译后修饰）功能， 光谱法 分析所获得的数据，并使用生物信息学方法协助解释结果。 在核心可用的管道。 培训项目负责人及其学生的最佳实践。 * 为参与分析设备使用和质量分析项目的学生提供培训 光谱数据。