Mass Spectrometry Core

项目摘要/摘要：质谱学核心(核心2) 质谱学核心(MSC)将与内布拉斯加州的项目负责人合作并提供支持 新蛋白质鉴定和验证中的分子靶点发现和开发中心 癌症治疗药物治疗的靶点。核心将利用新建立的基础设施和 生物技术中心蛋白质组学和代谢组学设施(PMF)提供的分析设备 内布拉斯加州林肯大学，它得到了内布拉斯加州研究倡议(NRI)的支持。这个 新的MSC核心将立即投入使用，以接受新中心项目负责人的项目。 请求为MSC提供支持的目标是为项目负责人提供全面的服务 并利用现有的仪器和计算资源引入新的分析能力。这个 MSC将努力保持最先进的水平，响应和解决项目目标的特定需求 由每个项目负责人在拨款建议书中描述。该中心将利用该中心的专业知识和技能 蛋白质组学和质谱学领域的领军人物阿尔瓦雷斯博士和共同研究员纳尔德雷特博士。 MSC的主要目标是： 协助项目负责人进行所需的实验设计和样品准备 拨款提案中描述的基于质谱学的实验。 处理和运行需要使用质量进行蛋白质鉴定和/或定量的样本 光谱分析和其他分析工具。 将使用MASS优化平台以实现新的PTM组学(翻译后修改)功能 光谱分析。 分析获取的数据，并使用生物信息学帮助解释结果 核心区有可用的管道。 对项目负责人及其学生进行最佳实践方面的教育。 将为参与分析设备和质量分析项目的学生提供培训 光谱数据。

Project Summary/Abstract: Mass Spectrometry Core (Core 2) The Mass Spectrometry Core (MSC) will collaborate with and support the project leaders within the Nebraska Center for Molecular Target Discovery and Development in the identification and validation of new protein targets for therapeutic drug treatment of cancers. The Core will utilize the newly established infrastructure and analytical equipment from the Proteomics and Metabolomics Facility (PMF) of the Center for Biotechnology at the University of Nebraska – Lincoln, which receives support from the Nebraska Research Initiative (NRI). The new MSC core will be immediately operational to accept projects from the Project Leaders of the new Center. The objective for the requested support for the MSC is to afford comprehensive services to the Project Leaders and to introduce new analytical capabilities using the instrumentation and computing resources available. The MSC will strive to remain state-of-the art, responding to and addressing the specific needs of the project aims described by each Project Leader in the grant proposal. The Center will use the expertise and skills of the leader Dr. Alvarez and Co-Investigator Dr. Naldrett in proteomics and mass spectrometry. The major goals of the MSC are:  to assist the Project Leaders with the experimental design and sample preparation required for the mass spectrometry-based experiments described in the grant proposal.  to process and run samples requiring protein identification and/or quantification using mass spectrometry and other analytical tools.  to optimize platforms for new PTMomics (post-translational modifications) capabilities using mass spectrometry.  to analyze the data acquired and assist with the interpretation of the results using the bioinformatic pipelines available in the core.  to educate the Project Leaders and their students on best practice.  to offer training of students involved in the projects on use of analytical equipment and analysis of mass spectrometry data.