Mass Spectrometry Core

质谱核心

• 批准号: 10117098
• 负责人: Sophie Alvarez
• 金额: $ 3.56万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-03-16 至 2023-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10117098
• 关键词: Address; Affect; Affinity Chromatography; Applications Grants; Biological Assay; Biotechnology; Blood; Breast Cancer cell line; Cell Line; Chemistry; Complex; Computer software; DNA-protein crosslink; Data; Development; Drug Delivery Systems; Drug Targeting; Encapsulated; Ensure; Equipment; Experimental Designs; Genetic; Goals; Human; Immunoprecipitation; Infrastructure; Knock-out; Label; Link; Liquid Chromatography; Mantle Cell Lymphoma; Mass Spectrum Analysis; Mediating; Methods; Molecular Target; Monitor; Nanostructures; Nebraska; Peptides; Pharmaceutical Preparations; Pharmacotherapy; Phenotype; Phosphopeptides; Phosphorylation; Phosphorylation Site; Plasma; Post-Translational Protein Processing; Preparation; Procedures; Process; Proteins; Proteome; Proteomics; Reagent; Regulation; Regulatory Pathway; Research; Research Personnel; Running; STAT3 gene; Sampling; Services; Signal Pathway; Signal Transduction; Site; Spectrometry, Mass, Electrospray Ionization; Stimulus; Students; TRAF6 gene; Technology; Therapeutic Intervention; U937 Cells; Ubiquitination; Universities; Validation; Wood material; analytical tool; base; bioinformatics pipeline; biomarker identification; cancer therapy; computing resources; cost effective; experience; experimental study; instrumentation; metabolomics; mutant; protein protein interaction; repaired; response; screening; skills; student training; tandem mass spectrometry; targeted treatment; therapeutic target; titanium dioxide; tool

Project Summary/Abstract: Mass Spectrometry Core (Core 2) The Mass Spectrometry Core (MSC) will collaborate with and support the project leaders within the Nebraska Center for Molecular Target Discovery and Development in the identification and validation of new protein targets for therapeutic drug treatment of cancers. The Core will utilize the newly established infrastructure and analytical equipment from the Proteomics and Metabolomics Facility (PMF) of the Center for Biotechnology at the University of Nebraska – Lincoln, which receives support from the Nebraska Research Initiative (NRI). The new MSC core will be immediately operational to accept projects from the Project Leaders of the new Center. The objective for the requested support for the MSC is to afford comprehensive services to the Project Leaders and to introduce new analytical capabilities using the instrumentation and computing resources available. The MSC will strive to remain state-of-the art, responding to and addressing the specific needs of the project aims described by each Project Leader in the grant proposal. The Center will use the expertise and skills of the leader Dr. Alvarez and Co-Investigator Dr. Naldrett in proteomics and mass spectrometry. The major goals of the MSC are:  to assist the Project Leaders with the experimental design and sample preparation required for the mass spectrometry-based experiments described in the grant proposal.  to process and run samples requiring protein identification and/or quantification using mass spectrometry and other analytical tools.  to optimize platforms for new PTMomics (post-translational modifications) capabilities using mass spectrometry.  to analyze the data acquired and assist with the interpretation of the results using the bioinformatic pipelines available in the core.  to educate the Project Leaders and their students on best practice.  to offer training of students involved in the projects on use of analytical equipment and analysis of mass spectrometry data.

项目摘要/摘要：质谱核心（核心 2） 质谱核心 (MSC) 将与内布拉斯加州的项目领导者合作并提供支持 分子靶标发现和开发中心，用于新蛋白质的鉴定和验证 癌症治疗药物治疗的靶点。核心区将利用新建立的基础设施和 生物技术中心蛋白质组学和代谢组学设施 (PMF) 的分析设备 内布拉斯加大学林肯分校获得内布拉斯加研究计划 (NRI) 的支持。这 新的 MSC 核心将立即投入运行，以接受新中心项目负责人的项目。 MSC 请求支持的目标是为项目负责人提供全面的服务 并利用可用的仪器和计算资源引入新的分析能力。这 MSC 将努力保持最先进的水平，响应并解决项目目标的具体需求 由每个项目负责人在拨款提案中描述。该中心将利用专家的专业知识和技能 蛋白质组学和质谱分析领域的领导者 Alvarez 博士和联合研究员 Naldrett 博士。 MSC 的主要目标是：  协助项目负责人进行实验设计和样品制备 拨款提案中描述的基于质谱的实验。 处理和运行需要使用质量进行蛋白质鉴定和/或定量的样品 光谱测定和其他分析工具。  使用质量优化新 PTMomics（翻译后修饰）功能的平台 光谱测定法。  使用生物信息学分析获得的数据并协助解释结果 核心中可用的管道。  对项目负责人及其学生进行最佳实践教育。  为参与项目的学生提供有关使用分析设备和质量分析的培训 光谱测量数据。