CACHET - Environmental Biomarkers Core

CACHET - 环境生物标志物核心

• 批准号: 10394644
• 负责人: Eric G. Pamer
• 金额: $ 28万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-30 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10394644
• 关键词: Abbreviations; Address; Bioinformatics; Biological; Biological Assay; Biological Markers; Biological Models; Blood; Chicago; Communities; Core Facility; Cost Sharing; Coupled; DNA; DNA sequencing; Data; Data Analyses; Data Reporting; Disease; Environment; Environmental Exposure; Environmental Health; Environmental Pollution; Exposure to; Family; Feces; Fourier transform ion cyclotron resonance; Funding; Gas Chromatography; Genetic Predisposition to Disease; Genomics; Goals; Health; Heavy Metals; Herbicides; High Pressure Liquid Chromatography; Human Microbiome; Illinois; Inductively Coupled Plasma Mass Spectrometry; Institutes; Institution; Investments; Liquid Chromatography; Liquid substance; MALDI-TOF Mass Spectrometry; Mass Spectrum Analysis; Measurement; Metagenomics; Mission; Monitor; National Institute of Environmental Health Sciences; Performance; Pesticides; Physiological; Plasma; Poison; Poly-fluoroalkyl substances; Population Dynamics; Predisposition; Proteomics; Reaction; Research; Research Personnel; Research Project Grants; Resources; Saliva; Sampling; Services; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Steroids; Time; Toxic Environmental Substances; Toxicogenomics; Universities; Volatile Fatty Acids; Water; air sampling; analytical method; base; bisphenol A; cost effective; design; epigenomics; gene environment interaction; health inequalities; health science research; host microbiome; human subject; instrumentation; member; metabolomics; microbial; microbiome sequencing; microbiota; oxidation; pressure; ranpirnase; research facility; small molecule; tandem mass spectrometry; transcriptomics; treatment response

1. ENVIRONMENTAL BIOMARKERS CORE (EBC): ABSTRACT The overall goal of the Environmental Biomarkers Core (EBC) is to provide bioanalytical services in support of ChicAgo Center for Health and EnvironmenT (CACHET) members conducting environmental health research at the University of Chicago (UofC) and University of Illinois at Chicago (UIC). By providing the necessary experimental expertise to assess a wide range of environmental biomarkers, the EBC directly contributes to CACHET’s mission to mitigate environmental health inequities within our communities. Toward that end, the EBC consists of two complimentary Sub-Cores: 1) a mass spectrometry-based Environmental Exposures Sub- Core that is P30-specific in services and resources available to members, and 2) a Biomarkers of Exposure Effects and Susceptibility Sub-Core that leverages existing institutional resources at UofC and UIC and streamlines services to members. The P30-specific Environmental Exposures Sub-Core at UIC will use mass spectrometry to assess toxic chemicals, heavy metals and metalloids, herbicides, pesticides, perfluoroalkyl substances, steroids and DNA oxidation products, among others, in both biological and environmental samples. New assays for environmental compounds will be developed and validated as needed by CACHET investigators. The Biomarkers of Exposure Effects and Susceptibility Sub-Core will leverage established institutional strengths in microbiome sequencing, metabolomics, proteomics, genomics, epigenomics and transcriptomics on the two campuses. Further, bioinformatics support for all CACHET PI-initiated research projects and Facility Core activities will be provided from established institutional cores at both partner institutions. For all of these services, CACHET members will enjoy priority access and subsidized rates for environmental health research projects provided by Center resources and leveraging institutional cost-sharing of intellectual expertise, management platforms and heavy investments in high-end instrumentation. These combined resources help address the EBC mission to provide qualitative and quantitative data regarding biomarkers of exposure, disease and therapeutic responses as well as levels of environmental contaminants in human subjects, model systems and environmental samples at subsidized rates and with priority access to Center members.

1.环境生物标志物核心（EBC）：摘要 环境生物标志物核心（EBC）的总体目标是提供生物分析服务， 芝加哥健康与环境中心（CACHET）成员在进行环境健康研究， 芝加哥大学（UofC）和伊利诺伊大学芝加哥分校（UIC）。通过提供必要的 实验专业知识，以评估广泛的环境生物标志物，EBC直接有助于 CACHET的使命是减轻我们社区内的环境健康不平等。为此， EBC由两个互补的子核心组成：1）基于质谱的环境暴露子核心， 核心是P30特定的服务和资源提供给成员，和2）暴露的生物标志物 影响和敏感性子核心，利用UofC和UIC的现有机构资源， 简化对会员的服务。UIC的P30特定环境暴露子核心将使用质量 光谱法评估有毒化学品、重金属和类金属、除草剂、杀虫剂、全氟烷基 在生物和环境样品中检测类固醇和DNA氧化产物等物质。 CACHET研究人员将根据需要开发和验证环境化合物的新检测方法。 暴露效应和易感性的生物标志物子核心将利用现有的机构优势 在微生物组测序、代谢组学、蛋白质组学、基因组学、表观基因组学和转录组学两个方面 校园。此外，生物信息学支持所有CACHET PI发起的研究项目和设施核心 活动将由两个伙伴机构的常设机构核心提供。对于所有这些服务， CACHET成员将享受环境卫生研究项目的优先准入和补贴费率 由中心资源提供，并利用知识专长、管理 平台和高端仪器的大量投资。这些综合资源有助于解决EBC问题 使命：提供关于暴露、疾病和治疗的生物标志物的定性和定量数据 响应以及人类受试者、模型系统和 环境样品的补贴率和优先访问中心成员。