Dopamine system as reporter of HIV status and inflammation in Meth abusers

多巴胺系统作为冰毒滥用者艾滋病毒状况和炎症的报告者

• 批准号: 10398692
• 负责人: Maria Cecilia Garibaldi Marcondes
• 金额: $ 0.63万
• 依托单位: SAN DIEGO BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-01 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10398692
• 关键词: Affinity; Age; Agonist; Behavior; Big Data; Biological Process; Blood Cells; Blood donor; Brain; CCR5 gene; Cell surface; Cells; Cellular Indexing of Transcriptomes and Epitopes by Sequencing; Central Nervous System Diseases; Cognitive; Computer software; Critical Thinking; DNA; DRD1 gene; DRD4 gene; Data Set; Dopamine; Dopamine Receptor; Drug Exposure; Environment; Exercise; Frequencies; Genes; Genetic; Genetic Polymorphism; Genetic Transcription; Genomics; Genotype; HIV; HIV Infections; HIV Receptors; Human; IL6 gene; Immune; Immune Targeting; Immune response; Immune system; In Vitro; Individual; Individual Differences; Infection; Inflammation; Inflammatory; Intake; Interleukin-10; Internships; Learning; Leukocytes; Life Expectancy; Link; METH abuser; Mathematics; Measures; Methamphetamine; Methods; Microglia; Modeling; Molecular; National Institute of Drug Abuse; National NeuroAids Tissue Consortium; Neuraxis; Neuroimmune; Neurologic; Neurological outcome; Neurotransmitters; Opioid; Organ; Outcome; Pathogenesis; Pattern; Performance; Peripheral; Peripheral Blood Mononuclear Cell; Pharmaceutical Preparations; Plasma; Predisposition; Protein Isoforms; RARG gene; RNA; Reporter; Rewards; Risk; Role; S100A8 gene; Sequence Alignment; Signal Transduction; Single Nucleotide Polymorphism; Specimen; Statistical Data Interpretation; Statistical Models; Students; System; Systems Biology; Testing; Training; Viral; Viral reservoir; Virus; Virus Diseases; Virus Latency; cell type; chronic infection; co-infection; cognitive performance; comorbidity; cytokine; dopamine system; dopamine transporter; drug abuser; experimental study; inflammatory marker; inflammatory milieu; innate immune function; insight; large datasets; macrophage; methamphetamine use; methamphetamine user; neuroAIDS; neuroinflammation; neuropathology; peripheral blood; polysubstance use; programs; receptor; social; substance use; substance user; summer internship; summer research; tool; viral DNA; viral RNA; virology; virtual

2021 NIDA Summer Research Internship Program Systems Biology Approaches to identify Biological Processes associated with Genomic effects of Dopamine in HIV-infected Macrophages Abstract Macrophages, microglia and other immune cells that are targets of Human Immunodeficiency Virus infection express receptors to dopamine, a neurotransmitter that is highly increased in the brain of stimulant drug abusers, including Methamphetamine. Our lab has performed single cell CITE-seq experiments to determine the role of dopamine in reactivating on maintaining the viral reservoir. The Summer Internship student will learn to analyze large sets of data to produce integrated models of biomolecular networks. The student will be virtually trained to administer systems biology strategies to big data and learn state-of-the-art methods for predicting gene networks expressing synchronized behaviors. At the end of the internship, the student will master a systems biology software, while exercising critical thinking, and produce results from our data set.

2021年NIDA暑期研究实习计划 系统生物学方法识别与基因组效应相关的生物学过程 HIV感染的巨噬细胞中的多巴胺 摘要 作为人类免疫缺陷病毒感染目标的巨噬细胞、小胶质细胞和其他免疫细胞 表达多巴胺的受体，这是一种在兴奋剂药物脑中高度增加的神经递质 吸毒者，包括冰毒。我们的实验室已经进行了单细胞CITE-SEQ实验来确定 多巴胺在维持病毒库的再激活中的作用。暑期实习生将学到 分析大量数据，以产生生物分子网络的集成模型。这名学生将成为 接受虚拟培训以管理大数据的系统生物学策略，并学习最先进的方法 预测表达同步行为的基因网络。在实习结束时，学生将 掌握系统生物学软件，同时进行批判性思维，并从我们的数据集产生结果。