Examining Mechanisms Underlying Drug-Associated Memory Erasure by Zeta-Inhibitory Peptide
检查 Zeta 抑制肽导致药物相关记忆擦除的机制
基本信息
- 批准号:10399321
- 负责人:
- 金额:$ 1.39万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-04-15 至 2024-01-31
- 项目状态:已结题
- 来源:
- 关键词:AMPA ReceptorsActinsAffectAnhedoniaAnimal ModelBehaviorBehavioralBindingBiologicalBrainBrain regionCellsChronicCocaineCocaine AbuseCocaine DependenceCountryCuesDataDendritesDiestrusDopamineDrug usageElectrophysiology (science)EstrusExcisionExhibitsExtinction (Psychology)FemaleFoodFundingFutureGlutamatesGoalsGonadal HormonesGrantHealthHealth Care CostsHumanImpairmentLearningLong-Term DepressionMediatingMemoryMetestrusMolecularMusN-MethylaspartateNucleus AccumbensOvarian hormoneParentsPeptidesPharmaceutical PreparationsPharmacological TreatmentPhosphotransferasesPhysiologicalPlayProcessProestrusProtein Kinase CProteinsPublic HealthPublishingReceptor SignalingRelapseRequest for ProposalsResearchRewardsRodentRoleSex DifferencesSex DifferentiationSignal TransductionStructureSubstance Use DisorderSubstance abuse problemSynapsesSynaptic plasticityTechniquesTherapeuticTreatment outcomeUnited StatesVertebral columnWithdrawalWomanWorkaddictionbehavioral responsecocaine relapsecocaine self-administrationcravingdensitydesigndrug cravingdrug of abuseexperimental studyglutamatergic signalingindexinginhibitor/antagonistkinase inhibitormalemenpolymerizationpre-clinicalpreventreceptorsexsmall molecular inhibitorsubstance use treatmenttraffickingtransmission process
项目摘要
.
Project Summary:
Cocaine abuse is a major public health problem in the United States with high rates of relapse and a lack to
pharmacological treatment options. We have recently demonstrated that zeta-inhibitory peptide (ZIP) infused
into the nucleus accumbens blocks cocaine reinstatement, an animal model of relapse. Further, ZIP's effects
persist up to 1 week after the peptide is cleared from the brain and ZIP does not alter food reinstatement.
Recent evidence has called the mechanism of action of ZIP into question. Therefore, the goal of this proposal
is to examine mechanisms by which ZIP affects cocaine-induced behavioral, synaptic and structural plasticity.
We, and others, have shown that cocaine self-administration leads to impaired NMDA-dependent long-term
depression (LTD) within the accumbens. Future learning, such as the extinction of drug-associated cues, could
be occluded without the ability to rescale these synapses. As LTD is dependent upon the removal of GluA2-
containing AMPARs, we hypothesize that ZIP may restore the capacity for LTD following cocaine by preventing
PKC-mediated removal of GluA2-containing AMPARs. Changes in AMPAR trafficking are dynamic and
alterations in structural plasticity, such as changes in spine density, provide a mechanism for persistent
changes at the receptor level. In fact, chronic cocaine has been shown to lead to structural plasticity within the
nucleus accumbens and manipulations that disrupt this structural plasticity decrease cocaine reward
behaviors, including reinstatement behavior. As structural plasticity is dependent upon actin polymerization and
cocaine has been shown to alter this process, we propose that ZIP may reverse cocaine-induced structural
plasticity via disruption of actin dynamics. As the ability of ZIP to eliminate cocaine reinstatement could provide
an avenue to designing potential therapeutics, understanding the mechanism by which these effects occur is
critical. Aim 1 focuses on determining how ZIP administration in the nucleus accumbens affects synaptic
plasticity and AMPAR trafficking. Additionally, this aim will determine whether the effects of ZIP on cocaine
reinstatement are dependent upon the ability to blunt LTD the necessity of the ability of ZIP to reverse this
plasticity in its behavioral effects. Aim 2 will focus on the potential affects of ZIP on cocaine-induced structural
plasticity. Aim 3 focuses on determining whether the effects of ZIP are dependent upon another atypical PKC,
PKCι/λ. Thus, the overall goal of the proposed experiments is to elucidate the mechanisms by which ZIP may
disrupt cocaine-induced plasticity at the level of the receptor and the dendrite.
。
项目概要:
可卡因滥用是美国的一个主要公共卫生问题,复发率很高,而且缺乏预防措施
药物治疗选择。我们最近证明了 zeta 抑制肽 (ZIP) 注入
进入伏隔核可阻止可卡因恢复,这是一种复发的动物模型。此外,ZIP 的效果
在肽从大脑中清除后,ZIP 可以持续长达 1 周,并且 ZIP 不会改变食物恢复。
最近的证据对 ZIP 的作用机制提出了质疑。因此,本提案的目标
目的是研究 ZIP 影响可卡因诱导的行为、突触和结构可塑性的机制。
我们和其他人已经证明,可卡因自我给药会导致长期 NMDA 依赖性受损
伏隔核内的凹陷(LTD)。未来的学习,例如与药物相关的线索的消失,可以
被遮挡而无法重新调整这些突触的大小。由于 LTD 取决于 GluA2- 的去除
含有 AMPAR,我们假设 ZIP 可以通过阻止可卡因恢复 LTD 的能力
PKC 介导的含 GluA2 AMPAR 的去除。 AMPAR 贩运的变化是动态的并且
结构可塑性的改变,例如脊柱密度的变化,提供了一种持久的机制
受体水平的变化。事实上,慢性可卡因已被证明会导致体内的结构可塑性。
伏隔核和破坏这种结构可塑性的操作会降低可卡因奖励
行为,包括恢复行为。由于结构可塑性取决于肌动蛋白聚合和
可卡因已被证明可以改变这一过程,我们建议 ZIP 可以逆转可卡因诱导的结构
通过破坏肌动蛋白动力学来实现可塑性。由于 ZIP 消除可卡因复效的能力可以提供
设计潜在疗法、了解这些效应发生机制的一种途径是
批判的。目标 1 侧重于确定伏隔核中的 ZIP 管理如何影响突触
可塑性和 AMPAR 贩运。此外,这一目标将确定 ZIP 对可卡因是否有影响
恢复取决于削弱 LTD 的能力 ZIP 扭转这种情况的能力的必要性
其行为影响的可塑性。目标 2 将重点关注 ZIP 对可卡因诱导的结构的潜在影响
可塑性。目标 3 侧重于确定 ZIP 的作用是否依赖于另一种非典型 PKC,
PKCι/λ。因此,所提出的实验的总体目标是阐明 ZIP 可能的机制
破坏可卡因诱导的受体和树突水平的可塑性。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
LISA A BRIAND其他文献
LISA A BRIAND的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('LISA A BRIAND', 18)}}的其他基金
The Building Research Independence by Developing Goals and Hands-on Experiences (BRIDGE) Program
通过制定目标和实践经验建立研究独立性(BRIDGE)计划
- 批准号:
10593235 - 财政年份:2023
- 资助金额:
$ 1.39万 - 项目类别:
Examining Mechanisms Underlying Drug-Associated Memory Erasure by Zeta-Inhibitory Peptide
检查 Zeta 抑制肽导致药物相关记忆擦除的机制
- 批准号:
10347308 - 财政年份:2019
- 资助金额:
$ 1.39万 - 项目类别:
Examining Mechanisms Underlying Drug-Associated Memory Erasure by Zeta-Inhibitory Peptide
检查 Zeta 抑制肽导致药物相关记忆擦除的机制
- 批准号:
9752721 - 财政年份:2019
- 资助金额:
$ 1.39万 - 项目类别:
Examining Mechanisms Underlying Drug-Associated Memory Erasure by Zeta-Inhibitory Peptide
检查 Zeta 抑制肽导致药物相关记忆擦除的机制
- 批准号:
9905503 - 财政年份:2019
- 资助金额:
$ 1.39万 - 项目类别:
Examining Mechanisms Underlying Drug-Associated Memory Erasure by Zeta-Inhibitory Peptide
检查 Zeta 抑制肽导致药物相关记忆擦除的机制
- 批准号:
10557811 - 财政年份:2019
- 资助金额:
$ 1.39万 - 项目类别:
AMPA Receptor Trafficking and Cocaine Reinstatement
AMPA 受体贩运和可卡因恢复
- 批准号:
9000679 - 财政年份:2015
- 资助金额:
$ 1.39万 - 项目类别:
AMPA Receptor Trafficking and Cocaine Reinstatement
AMPA 受体贩运和可卡因恢复
- 批准号:
8606840 - 财政年份:2013
- 资助金额:
$ 1.39万 - 项目类别:
AMPA Receptor Trafficking and Cocaine Reinstatement
AMPA 受体贩运和可卡因恢复
- 批准号:
8442554 - 财政年份:2013
- 资助金额:
$ 1.39万 - 项目类别:
The role of CREB in stress-induced reinstatement
CREB 在应激诱导恢复中的作用
- 批准号:
8232158 - 财政年份:2010
- 资助金额:
$ 1.39万 - 项目类别:
The role of CREB in stress-induced reinstatement
CREB 在应激诱导恢复中的作用
- 批准号:
7804995 - 财政年份:2010
- 资助金额:
$ 1.39万 - 项目类别:
相似海外基金
A novel motility system driven by two classes of bacterial actins MreB
由两类细菌肌动蛋白 MreB 驱动的新型运动系统
- 批准号:
22KJ2613 - 财政年份:2023
- 资助金额:
$ 1.39万 - 项目类别:
Grant-in-Aid for JSPS Fellows
The structural basis of plasmid segregation by bacterial actins
细菌肌动蛋白分离质粒的结构基础
- 批准号:
342887 - 财政年份:2016
- 资助金额:
$ 1.39万 - 项目类别:
Operating Grants
The structural basis for plasmid segregation by bacterial actins
细菌肌动蛋白分离质粒的结构基础
- 批准号:
278338 - 财政年份:2013
- 资助金额:
$ 1.39万 - 项目类别:
Operating Grants
Cytoplasmic Actins in Maintenance of Muscle Mitochondria
细胞质肌动蛋白在维持肌肉线粒体中的作用
- 批准号:
8505938 - 财政年份:2012
- 资助金额:
$ 1.39万 - 项目类别:
Differential Expression of the Diverse Plant Actins
多种植物肌动蛋白的差异表达
- 批准号:
7931495 - 财政年份:2009
- 资助金额:
$ 1.39万 - 项目类别:
Studies on how actins and microtubules are coordinated and its relevancy.
研究肌动蛋白和微管如何协调及其相关性。
- 批准号:
19390048 - 财政年份:2007
- 资助金额:
$ 1.39万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Interaction of myosin with monomeric actins
肌球蛋白与单体肌动蛋白的相互作用
- 批准号:
5311554 - 财政年份:2001
- 资助金额:
$ 1.39万 - 项目类别:
Priority Programmes
STRUCTURE/INTERACTIONS OF ACTINS AND ACTIN-BINDING PROTEIN
肌动蛋白和肌动蛋白结合蛋白的结构/相互作用
- 批准号:
6316669 - 财政年份:2000
- 资助金额:
$ 1.39万 - 项目类别:














{{item.name}}会员




