Shared Resource - Animal Models Shared Resource

摘要 动物模型是强大的癌症研究工具，对转化研究至关重要。的功能 动物模型共享资源（AMSR）是为了促进有效，经济，最先进的动物使用 用于癌症相关研究的性能。这是通过一个集中的资源来实现的， 美国实验动物科学协会认证，训练有素的兽医技术人员提供 为乔治敦隆巴迪综合癌症中心的成员提供动物转化研究服务 中心（LCCC）。一个重要的重点放在协助用户的设计和性能的动物 使用从斑马鱼到雌激素受体阳性大鼠的广泛模型进行研究 乳腺癌主要服务包括啮齿动物研究的各个方面，例如建立转基因 小鼠群体;给予致癌物;监测肿瘤生长;尸检时收集组织和肿瘤 以及施用药物、饮食或其它化合物。使用斑马鱼的研究包括产生基因敲除 或基因过表达模型，筛选药物毒性和进行异种移植实验 (i.e.,“zevatars”-斑马鱼化身）。还提供成像服务，以监测肿瘤的发生、进展 和对治疗的反应AMSR提供的成像服务包括高分辨率、体内、小尺寸、高分辨率和高分辨率。 使用Bruker 7特斯拉磁共振成像和光谱学的动物成像和图像分析 控制台、小动物超声（Vevo 660）工作站、X射线成像单元（Faxitron）和生物发光 和可见近红外光谱成像工作站（IVIS Lumina III）。AMSR位于 乔治城大学医学中心（GUMC）比较医学部（DCM）。DCM是一个 集中的，美国农业部注册的动物设施，由协会认可， 国际实验动物管理评估和认可组织（AAALAC），并已批准 在美国国立卫生研究院（NIH）存档的保证书。在2017财年，AMSR提供了 为来自所有四个研究项目（乳腺癌[BC]，癌症预防和 对照[CPC]、实验治疗[ET]和分子肿瘤学[MO]）。AMSR密切合作， 组织学与组织共享资源（HTSR）和生物统计学与生物信息学共享资源 （BBSR）。AMSR在资助期内资助了50份出版物。AMSR由克里斯托弗共同执导 Albanese博士、Leena Hilakivi-Clarke博士和Eric格拉斯哥博士，他们负责监督成像、啮齿动物和 斑马鱼成分。

ABSTRACT Animal models are powerful cancer research tools that are vital to translational research. The function of the Animal Models Shared Resource (AMSR) is to facilitate the efficient, economical, state-of-the-art use of animals for the performance of cancer-related studies. This is accomplished through a centralized resource in which American Association for Laboratory Animal Science–certified, highly trained veterinary technicians provide translational research services on animals to members of the Georgetown Lombardi Comprehensive Cancer Center (LCCC). A significant emphasis is placed on assisting users with the design and performance of animal studies using a wide range of models extending from zebrafish to an estrogen receptor-positive rat for studying breast cancer. Major services include all aspects of rodent studies, such as establishing genetically modified mouse colonies; administering carcinogens; monitoring tumor growth; collecting tissues and tumors at necropsy and administering drugs, diets or other compounds. Studies using zebrafish include generating gene knockout or gene overexpressing models, screening drugs for toxicity and conducting xenotransplantation experiments (i.e., “zevatars”—zebrafish avatar). Imaging services are also provided to monitor tumor initiation, progression and response to therapy. The imaging services provided by the AMSR include high-resolution, in vivo, small animal imaging and image analysis using a Bruker 7 tesla magnetic resonance imaging and spectroscopy console, a small animal ultrasound (Vevo 660) workstation, an x-ray imaging unit (Faxitron) and a bioluminescent and visible to near-infrared spectroscopic imaging workstation (IVIS Lumina III). The AMSR is located within Georgetown University Medical Center's (GUMC’s) Division of Comparative Medicine (DCM). The DCM is a centralized, U.S. Department of Agriculture-registered animal facility that is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International and that has an approved letter of assurance on file at the National Institutes of Health (NIH). In fiscal year 2017, the AMSR provided services to 24 LCCC members from all four Research Programs (Breast Cancer [BC], Cancer Prevention and Control [CPC], Experimental Therapeutics [ET] and Molecular Oncology [MO]). The AMSR works closely with the Histopathology & Tissue Shared Resource (HTSR) and the Biostatistics & Bioinformatics Shared Resource (BBSR). The AMSR supported 50 publications in the funding period. The AMSR is codirected by Christopher Albanese, PhD, Leena Hilakivi-Clarke, PhD, and Eric Glasgow, PhD, who oversee imaging, rodent and zebrafish components, respectively.