The role of mesoderm in regulating cell polarity and adhesion during neural crest cell migration. Supplement

中胚层在神经嵴细胞迁移过程中调节细胞极性和粘附的作用。

• 批准号: 10403890
• 负责人: Ezra Lencer
• 金额: $ 3.43万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2022-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10403890
• 关键词: Adhesions; Adult; Affect; Binding; Biosensor; Cell Adhesion; Cell Adhesion Molecules; Cell Polarity; Cells; Characteristics; Cleft Lip; Collagen; Complex; Congenital Megacolon; Data; Defect; Deposition; Development; Developmental Biology; Dorsal; Embryo; Environment; Etiology; Event; Extracellular Matrix; Fibronectins; Fishes; Genetic; Goals; Head; Hereditary Disease; Image; In Vitro; Integrins; Intrinsic factor; Knowledge; Laminin; Mediating; Mesoderm; Modification; Molecular; Movement; Muscle; Mutation; N-Cadherin; Neoplasm Metastasis; Neural Crest; Neural Crest Cell; Neural tube; Pathway interactions; Peptides; Peripheral; Phenocopy; Phenotype; Pigmentation physiologic function; Population; Regulation; Research; Role; Route; Series; Signal Transduction; Specific qualifier value; Stream; Suggestion; Surface; Testing; Tissues; Transplantation; Vertebrates; Zebrafish; bone; cell motility; experimental study; in vivo; insight; live cell imaging; migration; mutant; programs; rho; theories; trait; vertebrate embryos

Abstract Central to study of development is understanding how morphogenetic movements are regulated. This question has special bearing for the vertebrate neural crest, a multipotent population of cells that famously migrate in collective streams from the dorsal neural tube along conserved pathways during development. While much current theory focuses on cell autonomous factors regulating neural crest cell state, neural crest cell (NCC) migration is also known to require interactions and integration across different tissues. The overarching goal of this project is to understand how NCCs integrate internal cell state with external signals from the local cellular environment to coordinate directed migration. The proposed research will study this problem in zebrafish where trunk NCC migration depends critically on a population of somitic mesoderm called adaxial cells. Loss of adaxial cells results in NCC phenotypes suggestive of cell polarity and adhesion defects. Remarkably, adaxial cells also migrate during development, and NCC migration may be related to adaxial cell migration. Despite the importance of adaxial cells for NCC migration, the mechanisms underlying this interaction are unknown. The propose research will test the hypothesis that NCCs require adaxial cells for regulation of cell polarity, and that that this interaction is mediated through modifications to the extracellular matrix that occurs during adaxial cell migration. These data will provide insight into a regulatory mechanism controlling zebrafish NCC migration, a major morphogenetic event that occurs in every vertebrate embryo.

摘要 研究发育的核心是了解形态发生运动是如何调节的。这个问题 与脊椎动物的神经嵴有着特殊的关系，神经嵴是一种多能细胞群， 在发育过程中沿着沿着保守的通路从背神经管发出的集体流。虽然大部分 目前的理论主要集中在调节神经嵴细胞状态的细胞自主因子，神经嵴细胞（NCC）， 还已知迁移需要跨不同组织的相互作用和整合。的首要目标 这个项目的目的是了解网络控制中心如何将内部蜂窝状态与来自本地蜂窝的外部信号结合起来 环境协调定向迁移。拟议中的研究将在斑马鱼中研究这个问题 其中树干NCC迁移关键取决于称为近轴细胞的体节中胚层群体。损失 近轴细胞导致暗示细胞极性和粘附缺陷的NCC表型。值得注意的是， 细胞在发育过程中也迁移，NCC迁移可能与近轴细胞迁移有关。尽管 尽管近轴细胞对于NCC迁移的重要性，但是这种相互作用的机制尚不清楚。的 提出的研究将检验NCC需要近轴细胞来调节细胞极性的假设， 这种相互作用是通过近轴细胞生长过程中细胞外基质的修饰介导的。 迁移这些数据将提供深入了解控制斑马鱼NCC迁移的调节机制， 在每个脊椎动物胚胎中发生的主要形态发生事件。