COCA: Animal & Validation Core B

项目总结-动物和确认核心B 动物与验证核心B的首要目标和职责是提供 转基因啮齿类动物，具有向阿片类药物中心提供偶然药物的统一历史， COCA项目和验证试剂和转基因动物， 采用因此，核心B是核心C和项目之间COCA协同作用的关键组成部分 1-3在COCA中提供研究对象。这些啮齿动物包括年龄匹配的雄性和 接受可卡因、海洛因或蔗糖自我给药的雌性大鼠和小鼠， 根据每个项目的目标分发给各项目供随后使用。动物 核心B提供的转基因小鼠和大鼠，用于确定细胞类型和回路 与成瘾的神经生物学相关的特异性。统一的核心B提供了一个高效的 实验动物的轮换，创造项目之间的协同作用。除了啮齿动物， 核心B通过生产定制的AAV病毒载体促进协同作用， 病毒载体功效和细胞特异性表达，以及定量共定位分析。在 动物中心B将提供验证服务和转基因啮齿动物， 以下附加目标： 1.通过设计严格的培训方案，保持高质量的技术援助 严格遵守有关健康和安全的所有机构准则， 保证处理的一致性，并确保我们为我们的客户提供最健康的主题。 实验目的。 2.建立一个在COCA服务器上更新的集中记录保存系统， 数据的透明度和传播，以允许行为数据之间的比较， 项目进行的终点评价。 3.提供上传到公共和私有COCA的确认试剂数据库 服务器，以确定和统一与验证相关的常见问题的解决方案， 新试剂 4.作为MUSC和非MUSC个人有兴趣建立的培训中心 在他们的实验室里为大鼠和小鼠提供药物输送的偶然模型。 5.成为需要指导使用病毒载体质粒的人员的资源， 建立细胞类型特异性，验证转基因动物，并使用定量 共定位技术

PROJECT SUMMARY – Animal & Validation Core B The overarching goals and responsibilities of the Animal & Validation Core B are to provide transgenic rodents with uniform histories of contingent drug delivery to the Center on Opioid and Cocaine Addiction (COCA) projects and validate the reagents and transgenic animals that are used. As such, Core B is a critical component of COCA synergy between Core C, and Projects 1-3 in the COCA by providing research subjects. The rodents include age-matched male and female rats and mice that have undergone cocaine, heroin, or sucrose self-administration and that are distributed to the projects for subsequent use according to each projects aims. Animals supplied by Core B include transgenic mice and rats for determining cell-type and circuit specificity relevant to the neurobiology of addiction. The unified Core B provides an efficient rotation of laboratory animals that creates synergy between the projects. In addition to rodents, Core B promotes synergy through the production of custom AAV viral vectors, verification of viral vector efficacy and cell specific expression, and quantitative co-localization analysis. In providing the validation services and transgenic rodents, the Animal Core B will meet the following additional objectives: 1. Maintain a high quality of technical assistance through rigorous training protocols designed with strict adherence to all institutional guidelines regarding health and safety in order to guarantee consistency of handling and ensure we are generating the healthiest subjects for our experimental purposes. 2. Establish a centralized record keeping system updated on the COCA server that aids in the transparency and dissemination of data to allow for comparisons between behavioral data and the endpoint evaluations conducted by the projects. 3. Provide a database of confirmed reagents uploaded onto the public and private COCA servers in order to identify and unify solutions for common issues associated with validation of new reagents. 4. Serve as a training center for MUSC and non-MUSC personal interested in establishing contingent models of drug delivery in their laboratories for rats and mice. 5. Become a resource for personnel needing guidance on the use of viral vector plasmids, establishing cell-type specificity, validating transgenic animals, and using quantitative colocalization techniques.