The behavioral microstructure of a memory-guided food-caching behavior and its relationship to hippocampal replay

项目摘要 海马体是快速记忆形成和检索的关键部位，有广泛的文献记载 表示空间和导航变量的函数，但对它引导的方法知之甚少 行为。在许多物种中被识别为尖波纹波(SWR)事件的海马区活动爆发是 自SWRS以来，有望将海马区活动和行为功能联系起来的神经机制 海马体可以代表动物以前经历过的位置，或者在不久的将来将穿越的位置。 这一建议考虑了山雀的食物缓存行为，它们使用依赖于海马体的一种- 快照内存，用于指导缓存检索。这一行为非常有希望将海马体活动与 个人经历，这一直是确定SWR期间陈述性质的障碍 它们在以往研究中的作用。这些研究将全面阐述山雀是如何 行为受缓存站点内存的影响，确定SWR是否与特定内存相关 缓存行为的组件，并确定SWR是否代表以后要协助的各个缓存站点 取回。 我假设记忆引导的行为与随机觅食是不同的。我将使用视频跟踪来 测量山雀觅食行为的行为微观结构，包括姿势和凝视时间序列， 并开发了行为母题及其时间结构的量化模型。使用这些工具来 比较记忆引导和随机觅食行为，我将开发解码器，准确地指示如何 当行为表现为记忆引导时。 我假设，当行为是记忆时，SWR发生得更频繁，对行为的预测性更强。 引导者。我将使用电生理学在缓存过程中自由移动的山雀，将SWR与 行为的微观结构分析和行为策略的解读。 我假设SWR重新激活了以前各个缓存事件的表示，并且这 重新激活可改进该单个站点的后续检索。使用电生理记录 对于鸟类的缓存，我会将重新激活与检索行为的准确性联系起来。使用在线SWR检测和 抑制，我将进一步确定SWR是否会对以后的检索行为产生因果影响。

Project Summary The hippocampus is a critical site for rapid memory formation and retrieval, with extensively documented functions representing spatial and navigational variables, yet less is known of the means by which it guides behavior. Bursts of hippocampal activity identified in many species as sharp-wave ripple (SWR) events are promising neural mechanisms to link hippocampal activity and behavioral function, since during SWRs hippocampus can represent positions the animal previously experienced, or will traverse in the near future. This proposal considers the food-caching behaviors of chickadees, who use a hippocampal-dependent, one- shot memory to guide cache retrieval. This behavior is exceptionally promising to link hippocampal activity to individual experiences, which has been a barrier to determining the nature of representations during SWR and their function in previous studies. These studies will develop a comprehensive account of how chickadee behavior is affected by cache site memory, determine whether SWRs are related to memory-specific components of caching behavior, and determine whether SWRs represent individual cache sites to assist later retrieval. I hypothesize that memory-guided behavior is distinguishable from random foraging. I will use video tracking to measure the behavioral microstructure of chickadee caching behavior, including postural and gaze time series, and develop quantitative models of behavioral motifs and their temporal structure. Using these tools to compare memory-guided and random foraging behaviors, I will develop decoders that indicate precisely how and when behavior appears memory-guided. I hypothesize that SWRs occur more often, and are more predictive of behavior, when behavior is memory- guided. I will use electrophysiology in freely moving chickadees during caching to relate SWRs to the microstructural analysis of behavior and decoding of behavioral strategy. I hypothesize that SWRs reactivate representations of previous, individual caching events, and that this reactivation improves subsequent retrieval for that individual site. Using electrophysiological recordings in caching birds, I will relate reactivations to the accuracy of retrieval behavior. Using online SWR detection and suppression, I will further determine if SWRs causally affect later retrieval behavior.