KULMAP: Human Kidney, urinary tract and lung mapping center

KULMAP：人类肾脏、泌尿道和肺部绘图中心

• 批准号: 10413584
• 负责人: Sanjay Jain
• 金额: $ 10万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-25 至 2022-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10413584
• 关键词: 3-Dimensional; Ablation; Atlases; Blood Pressure; Blood Vessels; Chronic Kidney Failure; Clinical Trials; Data; Diabetes Mellitus; Disease; Epithelial Cells; Foundations; Goals; Homeostasis; Human; Human BioMolecular Atlas Program; Hypertension; Image; Immunofluorescence Microscopy; Kidney; Knowledge; Lung; Lymphatic; Lymphovascular; Maps; Metabolic; Microscopy; Nerve; Neuroeffector Junction; Obstruction; Organ; Pathologic; Resources; Role; Sensory; Site; Structure; System; Tissues; Urinary tract; Visualization; cell type; glomerular filtration; immunoregulation; innovation; interest; neurovascular; prevent; scaffold; technological innovation

Project Summary: The kidney nerves and lymphatics use blood vessels as conduits to reach their target sites of action including the vasculature, epithelial cells and interstitium. The lymphatics have important roles in immunomodulation and the kidney sensory and sympathetic nerves closely interact with target sites to regulate glomerular filtration, blood pressure and sense imbalances in metabolic derangements or physical obstruction. A three-dimensional (3D) atlas of regional and cellular distribution and neuroeffector sites in healthy kidneys is essential to understand the different pathological conditions and diseases due to their abnormal activities. However, these structures are not currently being studied in the kidney and pose a specific challenge due to the need for their assessment in meso (cm) and micro (microns) scale. The purpose of this supplement is to generate 3D-volumetric imaging data of sympathetic and sensory renal nerves and their relationship with the blood vessels, lymphatics and functionally important target cell types by leveraging resources of SPARC, HuBMAP and our technological innovations in applying 3D multiplexed immunofluorescence microscopy on large pieces of kidney tissue using Lightsheet microscopy. The neurovascular segmentation, scaffold building and quantitative visualization provided by SPARC will pave the way for broader efforts of similar goals for other HuBMAP organs. The importance of a spatial neurovascular atlas is of prime interest as targeted renal nerve ablation approaches are in clinical trials to treat hypertension and prevent progression of chronic kidney diseases.

项目摘要：肾神经和淋巴管使用血管作为管道到达其 作用靶点包括血管系统、上皮细胞和间质。淋巴管有 肾感觉神经和交感神经在免疫调节中的重要作用 以调节肾小球滤过、血压和感觉代谢失衡为靶点 精神错乱或身体障碍。区域和细胞的三维(3D)图集 健康肾脏的分布和神经效应部位对于理解不同的 因其异常活动而产生的病理状态和疾病。然而，这些结构是 目前还没有在肾脏中进行研究，并构成了一个特殊的挑战，因为需要他们的 中(厘米)和微(微米)尺度的评估。本附录的目的是生成 肾交感神经和感觉神经的三维容积成像数据及其与肾功能的关系 血管、淋巴管和功能上重要的靶细胞类型 SPARC、HuBMAP和我们在应用3D多重免疫荧光方面的技术创新 使用LightSheet显微镜对大片肾组织进行显微镜观察。神经血管 SPARC提供的分割、脚手架构建和定量可视化将为 为其他HuBMAP机构实现类似目标所作的更广泛努力。空间神经血管的重要性 阿特拉斯是最有兴趣的，因为靶向肾神经消融方法正在进行临床试验。 治疗高血压和防止慢性肾脏疾病的进展。