UMass Mouse Metabolic Phenotyping Center

项目摘要-摘要 麻省大学小鼠代谢表型中心（MMPC）涉及一个多学科的研究小组， 麻省大学谁执行一系列新颖和复杂的代谢，分析和功能实验，使用 用于研究可用于理解肥胖症的转基因小鼠的最先进设备， 糖尿病及其并发症。麻省大学MMPC的目标是提供全面的代谢和 使用主要涉及体内的独特和标准化技术对转基因小鼠进行功能表征 和生理设置，通过使用血清/组织样本的分析实验进行补充， 测量小鼠的胰岛和心血管功能。麻省大学MMPC由2个表型核心组成。的 Metabolism Core应用高胰岛素-正常血糖钳夹技术评估胰岛素作用和葡萄糖代谢 在单个器官中，高血糖钳夹以评估清醒小鼠中的胰腺β细胞功能，代谢 笼评估能量消耗，活动和食物摄入，1H-MRS测量身体组成，运动 使用跑步机的研究和盲法药物试验研究。分析和功能核心包括 3个子核心互补。分析子核心使用Luminex和Cobas化学分析仪， 激素、细胞因子和代谢物的血清/组织水平的高通量和多重测量， 并进行分子实验以检查胰岛素/代谢信号。Islet子核心执行优雅的 胰岛分离和离体和体外胰岛功能和组织学分析，以及分子分析。的 心血管亚核心使用最先进的Vevo 2100体内成像系统进行超声心动图检查 组织多普勒成像用于心脏结构和功能分析，遥测用于检查血压 和心率，评估血管和内皮功能的标准化技术，以及各种优雅的 心血管疾病的外科模型。用户提供的小鼠由动物核心监督， 处理小鼠的接收、检疫和圈养。人源化小鼠细胞移植与 评估子核心将通过提供人源化小鼠和优雅的技术来服务MMPC社区， 评估移植的人胰岛和干细胞衍生的β细胞在免疫缺陷小鼠中的体内功能。 最后，行政、财务和科学运作，研究和发展计划，以及网站和 数据库由管理核心管理。总的来说，马萨诸塞大学MMPC的首要目标是 使用最先进的设备提供小鼠的全面表征， 技术作为一个重要的科学资源，以支持国家MMPC财团计划的使命。

Project Summary - Abstract The UMass Mouse Metabolic Phenotyping Center (MMPC) involves a multidisciplinary group of investigators at UMass who perform an array of novel and sophisticated metabolic, analytical and functional experiments using state-of-the-art equipment for the purpose of investigating transgenic mice useful for understanding obesity, diabetes and its complications. The goal of the UMass MMPC is to provide comprehensive metabolic and functional characterization of transgenic mice using unique and standardized techniques mostly involving in vivo and physiological setting that are complemented by analytical experiments using serum/tissue samples and measure of islet and cardiovascular functions in mice. The UMass MMPC consists of 2 Phenotyping Cores. The Metabolism Core applies hyperinsulinemic-euglycemic clamp to assess insulin action and glucose metabolism in individual organs, hyperglycemic clamp to assess pancreatic β-cell functions in conscious mice, metabolic cages to assess energy expenditure, activity and food intake, 1H-MRS to measure body composition, exercise studies using treadmill, and blinded drug trial studies. The Analytical and Functional Core consists of the complementary 3 sub-Cores. Analytical sub-Core uses Luminex and Cobas Chemistry Analyzer to provide a high-throughput and multiplexed measurement of serum/tissue levels of hormones, cytokines and metabolites, and performs molecular experiments to examine insulin/metabolic signaling. The Islet sub-Core performs elegant islet isolation and ex vivo and in vitro analysis of islet function and histology, and molecular analysis. The Cardiovascular sub-Core uses state-of-the-art Vevo2100 In Vivo Imaging System to conduct echocardiography and tissue Doppler imaging for cardiac structure and function analyses, telemetry to examine blood pressure and heart rates, standardized techniques to assess vascular and endothelial functions, and various elegant surgical models of cardiovascular diseases. Mice provided by the users are overseen by the Animal Core that processes the receiving, quarantine, and housing of mice. The Humanized Mouse Cell Transplantation and Assessment sub-Core will service the MMPC community by offering humanized mice and elegant technique to assess in vivo function of transplanted human islets and stem cell-derived β-cells in immunodeficient mice. Lastly, administrative, financial and scientific operation, research & development program, and website & database are managed by the Administrative Core. Overall, the overarching goal of the UMass MMPC is to provide comprehensive characterization of mice using state-of-the-art equipment and elegant and standardized techniques as an important scientific resource to support the mission of the National MMPC consortium program.