UMass Mouse Metabolic Phenotyping Center
UMass Mouse Metabolic Phenotyping Center
批准号:
10411883
负责人:
JASON K KIM
金额:
$40.45万
依托单位国家:
美国
项目类别:
财政年份:
2011
资助国家:
美国
项目状态:
已结题
起止时间:
2011-09-16 至 2023-06-30
关键词:
AmericanAnimalsAwardBeta CellBiometryBlindedBlood PressureBody CompositionBody measure procedureCardiacCardiovascular DiseasesCardiovascular PhysiologyCardiovascular systemCell TransplantationCell physiologyChemistryClosure by clampCommunitiesComplementComplications of Diabetes MellitusConsciousDatabasesDiabetes MellitusEatingEchocardiographyEnergy MetabolismEnzyme-Linked Immunosorbent AssayEquipmentExerciseFee-for-Service PlansGlucoseGlucose ClampGoalsGrantHeart RateHistologicHistologyHormonesHousingHumanHyperglycemiaImageImmunodeficient MouseIn VitroIndividualInsulinLipidsM-Mode EchocardiographyMeasurementMeasuresMetabolicMetabolismMissionModelingMolecularMolecular AnalysisMonitorMorphologyMusObesityOperations ResearchOperative Surgical ProceduresOrganPharmaceutical PreparationsPhenotypePhysiologicalPrevalenceProceduresProcessProgram DevelopmentQuarantineResearchResearch PersonnelResolutionResourcesSerumServicesSignal PathwaySignal TransductionStandardizationStructureStructure of beta Cell of isletSurgical ModelsTechniquesTelemetryTissue SampleTissuesTrainingTransgenic MiceTransplantationUnited States National Institutes of HealthVascular Endotheliumchemokinecommunity centercytokinediabeticexperimental studyglucose metabolismheart imaginghumanized mousein vivoin vivo imaging systeminstrumentisletislet stem cellsmetabolic phenotypemultidisciplinarynoveloperationprogramsprotein metabolismresearch and developmenttooltreadmillweb site
中文摘要
项目摘要-摘要
英文摘要
Project Summary - Abstract
The UMass Mouse Metabolic Phenotyping Center (MMPC) involves a multidisciplinary group of investigators at
UMass who perform an array of novel and sophisticated metabolic, analytical and functional experiments using
state-of-the-art equipment for the purpose of investigating transgenic mice useful for understanding obesity,
diabetes and its complications. The goal of the UMass MMPC is to provide comprehensive metabolic and
functional characterization of transgenic mice using unique and standardized techniques mostly involving in vivo
and physiological setting that are complemented by analytical experiments using serum/tissue samples and
measure of islet and cardiovascular functions in mice. The UMass MMPC consists of 2 Phenotyping Cores. The
Metabolism Core applies hyperinsulinemic-euglycemic clamp to assess insulin action and glucose metabolism
in individual organs, hyperglycemic clamp to assess pancreatic β-cell functions in conscious mice, metabolic
cages to assess energy expenditure, activity and food intake, 1H-MRS to measure body composition, exercise
studies using treadmill, and blinded drug trial studies. The Analytical and Functional Core consists of the
complementary 3 sub-Cores. Analytical sub-Core uses Luminex and Cobas Chemistry Analyzer to provide a
high-throughput and multiplexed measurement of serum/tissue levels of hormones, cytokines and metabolites,
and performs molecular experiments to examine insulin/metabolic signaling. The Islet sub-Core performs elegant
islet isolation and ex vivo and in vitro analysis of islet function and histology, and molecular analysis. The
Cardiovascular sub-Core uses state-of-the-art Vevo2100 In Vivo Imaging System to conduct echocardiography
and tissue Doppler imaging for cardiac structure and function analyses, telemetry to examine blood pressure
and heart rates, standardized techniques to assess vascular and endothelial functions, and various elegant
surgical models of cardiovascular diseases. Mice provided by the users are overseen by the Animal Core that
processes the receiving, quarantine, and housing of mice. The Humanized Mouse Cell Transplantation and
Assessment sub-Core will service the MMPC community by offering humanized mice and elegant technique to
assess in vivo function of transplanted human islets and stem cell-derived β-cells in immunodeficient mice.
Lastly, administrative, financial and scientific operation, research & development program, and website &
database are managed by the Administrative Core. Overall, the overarching goal of the UMass MMPC is to
provide comprehensive characterization of mice using state-of-the-art equipment and elegant and standardized
techniques as an important scientific resource to support the mission of the National MMPC consortium program.
期刊论文(6)
专著(0)
科研奖励(0)
会议论文
DOI:
10.3390/microorganisms9112357
发表时间:
2021-11-15
期刊:
Microorganisms
影响因子:
4.5
作者:
[Walter DL, Thuma JR, Malgor R, Schwartz FL, McCall KD, Coschigano KT]
通讯作者:
Coschigano KT
Role of Interferon-Gamma / Interleukin-12 Axis in Metabolic Liver Disease
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批准号:10735419
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项目类别:
-
资助金额:$49.0万
-
财政年份:2023
-
负责人:JASON K KIM
-
依托单位:
UMass Mouse Metabolic Phenotyping Center
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批准号:9517854
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项目类别:
-
资助金额:$83.67万
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财政年份:2011
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负责人:JASON K KIM
-
依托单位:
UMass Mouse Metabolic Phenotyping Center
-
批准号:8334799
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项目类别:
-
资助金额:$43.25万
-
财政年份:2011
-
负责人:JASON K KIM
-
依托单位:
UMass Mouse Metabolic Phenotyping Center
-
批准号:8517702
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项目类别:
-
资助金额:$41.73万
-
财政年份:2011
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负责人:JASON K KIM
-
依托单位:
UMass Mouse Metabolic Phenotyping Center
-
批准号:8708049
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项目类别:
-
资助金额:$43.25万
-
财政年份:2011
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负责人:JASON K KIM
-
依托单位:
UMass Mouse Metabolic Phenotyping Center
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批准号:8193746
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项目类别:
-
资助金额:$43.25万
-
财政年份:2011
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负责人:JASON K KIM
-
依托单位:
Interleukin-10 and Regulation of Skeletal Muscle Insulin Action
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批准号:8325167
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项目类别:
-
资助金额:$32.25万
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财政年份:2008
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负责人:JASON K KIM
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依托单位:
Interleukin-10 and Regulation of Skeletal Muscle Insulin Action
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批准号:8138353
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项目类别:
-
资助金额:$32.25万
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财政年份:2008
-
负责人:JASON K KIM
-
依托单位:
Interleukin-10 and Regulation of Skeletal Muscle Insulin Action
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批准号:7688617
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项目类别:
-
资助金额:$32.82万
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财政年份:2008
-
负责人:JASON K KIM
-
依托单位:
Interleukin-10 and Regulation of Skeletal Muscle Insulin Action
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批准号:7908877
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项目类别:
-
资助金额:$32.57万
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财政年份:2008
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负责人:JASON K KIM
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依托单位:
Metabolism Core
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批准号:8708056
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项目类别:
-
资助金额:$24.68万
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财政年份:--
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负责人:JASON K KIM
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依托单位:
UMass Mouse Metabolic Phenotyping Center - Metabolism Core
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批准号:9174713
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项目类别:
-
资助金额:$32.15万
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财政年份:--
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负责人:JASON K KIM
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依托单位:
Administrative Core
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批准号:8517704
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项目类别:
-
资助金额:$12.9万
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财政年份:--
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负责人:JASON K KIM
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依托单位:
Metabolism Core
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批准号:8517708
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项目类别:
-
资助金额:$23.81万
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财政年份:--
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负责人:JASON K KIM
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依托单位:
Administrative Core
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批准号:8381200
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项目类别:
-
资助金额:$7.19万
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财政年份:--
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负责人:JASON K KIM
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依托单位:
Analytical Core
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批准号:8708057
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项目类别:
-
资助金额:$7.06万
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财政年份:--
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负责人:JASON K KIM
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依托单位:
Administrative Core
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批准号:8708054
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项目类别:
-
资助金额:$6.09万
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财政年份:--
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负责人:JASON K KIM
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依托单位:
Animal Care Core
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批准号:8880191
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项目类别:
-
资助金额:$5.43万
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财政年份:--
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负责人:JASON K KIM
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依托单位:
Metabolism Core
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批准号:8204157
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项目类别:
-
资助金额:$24.68万
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财政年份:--
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负责人:JASON K KIM
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依托单位:
Animal Care Core
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批准号:8381202
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项目类别:
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资助金额:$5.43万
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财政年份:--
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负责人:JASON K KIM
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依托单位:
海外基金