UMass Mouse Metabolic Phenotyping Center

项目摘要-摘要 华盛顿大学小鼠代谢表型中心(MMPC)涉及一个多学科的研究小组，位于 执行一系列新颖和复杂的代谢、分析和功能实验，使用 最先进的设备，用于研究有助于了解肥胖的转基因小鼠， 糖尿病及其并发症。UMASS MMPC的目标是提供全面的代谢和 利用独特的标准化技术对转基因小鼠进行功能鉴定，主要涉及体内 和生理环境，并通过使用血清/组织样本和 测量小鼠的胰岛和心血管功能。UMassMMPC由2个表型核心组成。这个 新陈代谢核心应用高胰岛素-正常血糖钳夹评估胰岛素作用和葡萄糖代谢 在个别器官，高血糖钳夹评估清醒小鼠胰腺β细胞功能，代谢 笼子用于评估能量消耗、活动和食物摄入量，1H-MRS用于测量身体成分、运动 使用跑步机的研究，以及盲目药物试验研究。分析和功能核心由 互补的3个子核。分析子核心使用Luminex和Cobas化学分析仪提供 高通量和多路测量血清/组织激素、细胞因子和代谢物水平， 并进行分子实验来检查胰岛素/代谢信号。小岛次核的表现非常出色 胰岛分离，体外及体外胰岛功能和组织学分析，以及分子分析。这个 心血管亚核使用最先进的Vevo2100 in Vivo成像系统进行超声心动图 组织多普勒成像用于心脏结构和功能分析，遥测用于检查血压 和心率，评估血管和内皮功能的标准化技术，以及各种优雅的 心血管疾病的外科模型。由用户提供的鼠标由Animal Core监督 处理老鼠的接收、隔离和安置。人源化小鼠细胞移植和 评估子核心将通过提供人性化的鼠标和优雅的技术来服务MMPC社区 评估免疫缺陷小鼠移植的人胰岛和干细胞来源的β细胞的体内功能。 最后，行政、财务和科学运作、研发计划和网站& 数据库由管理核心管理。总体而言，马萨诸塞州MMPC的首要目标是 使用最先进的设备和优雅而标准化的设备提供全面的老鼠特征 技术是支持国家MMPC联盟计划使命的重要科学资源。

Project Summary - Abstract The UMass Mouse Metabolic Phenotyping Center (MMPC) involves a multidisciplinary group of investigators at UMass who perform an array of novel and sophisticated metabolic, analytical and functional experiments using state-of-the-art equipment for the purpose of investigating transgenic mice useful for understanding obesity, diabetes and its complications. The goal of the UMass MMPC is to provide comprehensive metabolic and functional characterization of transgenic mice using unique and standardized techniques mostly involving in vivo and physiological setting that are complemented by analytical experiments using serum/tissue samples and measure of islet and cardiovascular functions in mice. The UMass MMPC consists of 2 Phenotyping Cores. The Metabolism Core applies hyperinsulinemic-euglycemic clamp to assess insulin action and glucose metabolism in individual organs, hyperglycemic clamp to assess pancreatic β-cell functions in conscious mice, metabolic cages to assess energy expenditure, activity and food intake, 1H-MRS to measure body composition, exercise studies using treadmill, and blinded drug trial studies. The Analytical and Functional Core consists of the complementary 3 sub-Cores. Analytical sub-Core uses Luminex and Cobas Chemistry Analyzer to provide a high-throughput and multiplexed measurement of serum/tissue levels of hormones, cytokines and metabolites, and performs molecular experiments to examine insulin/metabolic signaling. The Islet sub-Core performs elegant islet isolation and ex vivo and in vitro analysis of islet function and histology, and molecular analysis. The Cardiovascular sub-Core uses state-of-the-art Vevo2100 In Vivo Imaging System to conduct echocardiography and tissue Doppler imaging for cardiac structure and function analyses, telemetry to examine blood pressure and heart rates, standardized techniques to assess vascular and endothelial functions, and various elegant surgical models of cardiovascular diseases. Mice provided by the users are overseen by the Animal Core that processes the receiving, quarantine, and housing of mice. The Humanized Mouse Cell Transplantation and Assessment sub-Core will service the MMPC community by offering humanized mice and elegant technique to assess in vivo function of transplanted human islets and stem cell-derived β-cells in immunodeficient mice. Lastly, administrative, financial and scientific operation, research & development program, and website & database are managed by the Administrative Core. Overall, the overarching goal of the UMass MMPC is to provide comprehensive characterization of mice using state-of-the-art equipment and elegant and standardized techniques as an important scientific resource to support the mission of the National MMPC consortium program.