UMass Mouse Metabolic Phenotyping Center

描述（由申请人提供）：马萨诸塞大学小鼠代谢表型中心由马萨诸塞大学的多学科研究人员组成，他们使用最先进的设备进行一系列新颖和复杂的代谢实验，目的是研究对了解糖尿病及其并发症有用的转基因小鼠。马萨诸塞大学MMPC的目标是使用独特和标准化的技术提供转基因小鼠的全面代谢特征，主要涉及体内和生理环境，并辅以使用血清/组织样本的分析实验和使用非侵入性方法测量小鼠的心血管并发症。UMass MMPC由3个表型核心组成：1)代谢核心使用高胰岛素-正糖钳来评估个体器官的胰岛素敏感性和葡萄糖代谢，高血糖钳来评估有意识小鼠的胰腺β细胞功能，代谢笼来评估能量消耗，活动和食物摄入，1H-MRS测量小鼠的脂肪/瘦/水质量，2)分析核心使用Luminex。Meso Scale Discovery和Cobas临床化学分析仪提供高通量和多路测量血清/组织激素，细胞因子和代谢物水平，进行分子实验以检查胰岛素信号，并进行组织学研究以检查组织。3)心血管并发症Core使用Vevo2100体内成像系统进行超声心动图和组织多普勒成像。以及CODA血压装置，用于检查与糖尿病相关的心血管异常。用户提供的小鼠由动物护理中心负责监督，该中心负责处理小鼠的接收、隔离和饲养。最后，财务和行政运作、研发计划、网站和数据库由行政核心管理。总的来说，马萨诸塞大学MMPC的目标是成为一个由美国国立卫生研究院资助的国家中心，提供优雅和标准化的技术和科学专业知识，使用收费的服务结构来表征转基因小鼠的代谢和器官功能表型，并为糖尿病及其并发症的机制提供重要的见解。

DESCRIPTION (provided by applicant): The UMass Mouse Metabolic Phenotyping Center involves a multidisciplinary group of investigators at UMass who perform an array of novel and sophisticated metabolic experiments using state-of-the-art equipment for the purpose of investigating transgenic mice useful for understanding diabetes and its complications. The goal of the UMass MMPC is to provide comprehensive metabolic characterization of transgenic mice using unique and standardized techniques mostly involving, in vivo and physiological setting that are complemented by analytical experiments using serum/tissue samples and measure of cardiovascular complications using non-invasive procedures in mice. The UMass MMPC consists of 3 Phenotyping Cores: 1) Metabolism Core applies hyperinsullnemic-euglycemic clamp to assess insulin sensitivity and glucose metabolism in individual organs, hyperglycemic clamp to assess pancreatic beta-cell function in conscious mice, metabolic cages to assess energy expenditure, activity and food intake, and 1H-MRS to measure fat/lean/water mass in mice, 2) Analytical Core uses Luminex. Meso Scale Discovery, and Cobas Clinical Chemistry Analyzer to provide a high-throughput and multiplexed measurement of serum/tissue levels of hormones, cytokines and metabolites, performs molecular experiments to examine insulin signaling, and conducts histological studies to examine tissues, 3) Cardiovascular Complications Core uses Vevo2100 In Vivo Imaging System to conduct echocardiography and tissue Doppler imaging, and CODA blood pressure device to examine cardiovascular abnormalities associated with diabetes. Mice provided by the users are overseen by the Animal Care Core that processes the receiving, quarantine, and housing of mice. Lastly, financial and administrative operation, research & development program, and website & database are managed by the Administrative Core. Overall, the aim of the UMass MMPC is to become an NIH-funded national center that offers elegant and standardized techniques and scientific expertise using a fee-for-service structure to characterize the metabolic and organ function phenotypes of transgenic mice and provide important insights into the mechanism of diabetes and its complications. PUBLIC HEALTH RELEVANCE: Prevalence of diabetes is increasing at an alarming rate, and by year 2025. One out of 3 Americans is expected to be diabetic. Using established expertise, elegant experimental procedures, and state-of-the-art instruments, our goal is to understand how diabetes develops and to identify a cure to treat obesity and diabetes.