UMass Mouse Metabolic Phenotyping Center

描述（由申请人提供）：麻省大学小鼠代谢表型中心涉及麻省大学的一个多学科研究小组，他们使用最先进的设备进行一系列新颖复杂的代谢实验，目的是研究有助于了解糖尿病及其并发症的转基因小鼠。UMass MMPC的目标是使用主要涉及体内和生理环境的独特和标准化技术提供转基因小鼠的全面代谢表征，并通过使用血清/组织样本的分析实验和使用小鼠非侵入性程序测量心血管并发症进行补充。UMass MMPC由3个表型分析核心组成：1）代谢核心应用高胰岛素-正常血糖钳夹技术评估单个器官的胰岛素敏感性和葡萄糖代谢，高血糖钳夹技术评估清醒小鼠的胰腺β细胞功能，代谢笼评估能量消耗、活动和摄食量，1H-MRS测量小鼠的脂肪/瘦肉/水分质量，2）分析核心使用Luminex。Meso Scale Discovery和Cobas临床化学分析仪提供激素、细胞因子和代谢物的血清/组织水平的高通量和多路测量，进行分子实验以检查胰岛素信号传导，并进行组织学研究以检查组织，3）心血管并发症核心使用Vevo 2100体内成像系统进行超声心动图和组织多普勒成像，和CODA血压设备，以检查与糖尿病相关的心血管异常。用户提供的小鼠由动物护理中心监督，该中心负责处理小鼠的接收、检疫和圈养。最后，财务和行政运作，研究与开发计划，网站和数据库由行政核心管理。总的来说，麻省大学MMPC的目标是成为NIH资助的国家中心，提供优雅和标准化的技术和科学专业知识，使用收费服务结构来表征转基因小鼠的代谢和器官功能表型，并提供重要的见解糖尿病及其并发症的机制。 公共卫生相关性：到2025年，糖尿病的患病率正以惊人的速度增加。每三个美国人中就有一个是糖尿病患者。利用成熟的专业知识，优雅的实验程序和最先进的仪器，我们的目标是了解糖尿病如何发展，并确定治疗肥胖和糖尿病的方法。

DESCRIPTION (provided by applicant): The UMass Mouse Metabolic Phenotyping Center involves a multidisciplinary group of investigators at UMass who perform an array of novel and sophisticated metabolic experiments using state-of-the-art equipment for the purpose of investigating transgenic mice useful for understanding diabetes and its complications. The goal of the UMass MMPC is to provide comprehensive metabolic characterization of transgenic mice using unique and standardized techniques mostly involving, in vivo and physiological setting that are complemented by analytical experiments using serum/tissue samples and measure of cardiovascular complications using non-invasive procedures in mice. The UMass MMPC consists of 3 Phenotyping Cores: 1) Metabolism Core applies hyperinsullnemic-euglycemic clamp to assess insulin sensitivity and glucose metabolism in individual organs, hyperglycemic clamp to assess pancreatic beta-cell function in conscious mice, metabolic cages to assess energy expenditure, activity and food intake, and 1H-MRS to measure fat/lean/water mass in mice, 2) Analytical Core uses Luminex. Meso Scale Discovery, and Cobas Clinical Chemistry Analyzer to provide a high-throughput and multiplexed measurement of serum/tissue levels of hormones, cytokines and metabolites, performs molecular experiments to examine insulin signaling, and conducts histological studies to examine tissues, 3) Cardiovascular Complications Core uses Vevo2100 In Vivo Imaging System to conduct echocardiography and tissue Doppler imaging, and CODA blood pressure device to examine cardiovascular abnormalities associated with diabetes. Mice provided by the users are overseen by the Animal Care Core that processes the receiving, quarantine, and housing of mice. Lastly, financial and administrative operation, research & development program, and website & database are managed by the Administrative Core. Overall, the aim of the UMass MMPC is to become an NIH-funded national center that offers elegant and standardized techniques and scientific expertise using a fee-for-service structure to characterize the metabolic and organ function phenotypes of transgenic mice and provide important insights into the mechanism of diabetes and its complications. PUBLIC HEALTH RELEVANCE: Prevalence of diabetes is increasing at an alarming rate, and by year 2025. One out of 3 Americans is expected to be diabetic. Using established expertise, elegant experimental procedures, and state-of-the-art instruments, our goal is to understand how diabetes develops and to identify a cure to treat obesity and diabetes.