Genetics of stroke vulnerability in C57BL/6 mouse substrains

C57BL/6 小鼠亚系中风易感性的遗传学

• 批准号: 10407577
• 负责人: Megan Kathleen Mulligan
• 金额: $ 28.58万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-15 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10407577
• 关键词: Address; Affect; Automobile Driving; Bioinformatics; C57BL/6 Mouse; CRISPR/Cas technology; Chromium; Comparative Genomic Analysis; Confidence Intervals; Data; Detection; Dominant Genetic Conditions; Exhibits; Experimental Models; Future; General Population; Genes; Genetic; Genetic Determinism; Genome; Genomics; Genotype; Heritability; Human; Individual; Infarction; Inherited; Intervention; Ischemia; Link; Maps; Mediating; Methodology; Molecular Biology Techniques; Mouse Strains; Mus; Mutation; Parents; Phenotype; Physiology; Population; Prevention; Quantitative Trait Loci; Resources; Severities; Source; Stroke; Structure; Technology; Testing; Therapeutic; United States National Institutes of Health; Validation; Variant; biomarker panel; causal variant; cohort; coisogenic; comparative; design; gene discovery; gene network; genetic variant; genome editing; genome sequencing; genome-wide; genomic locus; human disease; novel; repaired; sex; stroke risk; tool; translational potential; whole genome

Stroke remains a significant human disease, and understanding the genetic factors that contribute to its impact on specific individuals will ultimately be critical in targeting interventions. Studies in human populations generally address factors affecting the risk of stroke occurrence. Impacts on stroke severity are readily examined in experimental models, and parallel mapping of vulnerability loci in mouse and human populations support the translational potential of such analyses. We have recently identified marked divergence of stroke vulnerability among closely related substrains of the C57BL/6 mouse. Smaller infarcts are seen in the J and ByJ substrains, the latter of which originated early in the N lineage. In contrast, larger infarcts are observed in subsequently derived NCrSlc, NCrl and NJ substrains. Preliminary results establish that the larger infarct phenotype is inherited as a dominant trait, indicating a single causal mutation. These nearly coisogenic substrain populations contain only a limited pool of segregating genetic variants that could underlie the difference in stroke severity. We propose to identify the gene variant that modulates stroke vulnerability among C57BL/6 substrains. Aim 1 will generate comprehensive sequence data for ByJ, NCrSlc and NCrl substrains, for which such resources are not yet available. Since ByJ and NCrSlc are the most closely related substrains to differ in stroke vulnerability, this will define the minimum list of candidate variants. Aim 2 will use high-coverage genomic sequences and a dense marker panel already available for J and NJ substrains to map the locus impacting infarct volume in reciprocal populations of JxNJ and NJxJ F2 intercross progeny subjected to permanent focal ischemia. This locus will be confirmed using F2 crosses of ByJ and NCrl substrains. Aim 3, using CRISPR- Cas9 methodology to repair each candidate variant, will establish the single genetic factor that is the main source of variation in stroke vulnerability among C57BL/6 substrains.

中风仍然是一种重要的人类疾病，了解遗传因素