Mouse Support Core

鼠标支撑核心

• 批准号: 10407611
• 负责人: Ulrike Lorenz
• 金额: $ 39.77万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10407611
• 关键词: Address; Animal Model; Animals; Blood Vessels; Breeding; Cardiovascular system; Cells; Communities; Databases; Disease; Ensure; Equipment and supply inventories; Funding; Genes; Genotype; Goals; Individual; Inflammation; Inflammatory; Knock-out; Knockout Mice; LoxP-flanked allele; Maintenance; Mediating; Mission; Modeling; Mouse Strains; Mus; Mutation; Physiology; Proteins; Research Personnel; STK11 gene; Site; Source; Support System; Time; Tissues; Transgenes; Transgenic Mice; Transgenic Organisms; Variant; Work; animal resource; base; cost; genetic regulatory protein; human disease; innovation; mouse model; mutant; overexpression; programs; success; tool

CORE B PROJECT SUMMARY The primary goals of this Program Project application are to understand the function of pannexin channels in normal physiology, as well as well as under conditions of cardiovascular and inflammatory diseases. This Mouse Support Core/Core B will serve all projects in the following ways: (1) This core will provide the husbandry, genotyping and basic crossing of conditional Panx1 knockout mice to specific Cre strains; specifically, the Panx1fl/fl mice crossed to at least five different Cre strains that are relevant to studying cardiovascular and inflammatory diseases. (2) This Core will also help generate additional transgenic Panx1 mice for tissue-specific expression of Panx1-WT and variants. (3) The Core will generate Cre-mediated tissue-speficic knockouts of the Panx1-regulatory proteins Sik1 and LKB1. The key benefits of this Mouse Support Core are that it will help consolidate the breeding and mouse maintenance, save time and cost, and avoid variations due to colonies individually maintained by the four project leaders. As the previous funding period has shown, having a dedicated core to cross the various Cre strains will be a huge advantage to all four projects. Moreover, the innovative transgenic mouse models overexpressing wild type or mutant forms of Panx1 will help answer key questions posed in the four main Projects in additional unique ways. Collectively, these mouse strains/models and analyses tools generated are expected to be fundamentally important in advancing our work to human disease, and will also help the larger community of researchers studying pannexin channel function pertaining to many human diseases.

核心B项目摘要 该计划项目应用程序的主要目标是了解Pannexin渠道的功能 正常的生理学以及在心血管和炎症性疾病的条件下。这只鼠标 支持核心/核心B将以以下方式为所有项目提供服务：（1）此核心将提供饲养， 条件panx1基因敲除小鼠与特定Cre菌株的基因分型和基本交叉；具体来说， PANX1FL/FL小鼠至少与研究心血管和研究和 炎症性疾病。 （2）该核心还将有助于生成额外的转基因PANX1小鼠用于组织特异性 panx1-wt和变体的表达。 （3）核心将产生CRE介导的组织细分敲除 PANX1调节蛋白SIK1和LKB1。该鼠标支撑核心的关键好处是它将有所帮助 巩固繁殖和鼠标维护，节省时间和成本，并避免因菌落而变化 由四个项目负责人单独维护。正如上一个资金期所表明的那样，拥有一个专用 跨越各种CRE应变的核心将是所有四个项目的巨大优势。而且，创新 过表达野生型或突变形式的转基因鼠标模型将有助于回答关键问题 以其他独特的方式摆在四个主要项目中。这些小鼠菌株/模型和分析总共 预计产生的工具对于将我们的工作推进到人类疾病至关重要，并且会将 还可以帮助研究泛毒素渠道功能的较大的研究人员与许多人有关 疾病。