Mouse Support Core

核心B项目摘要 本计划项目申请的主要目标是了解PAnnexin通道在 正常生理，以及在心血管疾病和炎症性疾病条件下。这只鼠标 支持核心/核心B将通过以下方式为所有项目提供服务：(1)该核心将提供畜牧业， 条件Panx1基因敲除小鼠与特定Cre菌株的基因分型和基本杂交；具体地说， Panx1fl/fl小鼠与至少五个不同的Cre品系杂交，这些品系与研究心血管和 炎症性疾病。(2)这个核心还将帮助产生更多针对组织特异性的转基因Panx1小鼠 Panx1-WT及其变异体的表达。(3)核心将产生Cre介导的组织特异性敲除 Panx1-调节蛋白SIK1和LKB1。此鼠标支持核心的主要好处是它将有助于 加强繁育和老鼠维护，节省时间和成本，避免因群体而变异 由四位项目负责人单独维护。正如上一个资助期所显示的那样，拥有专门的 跨越各种CRE菌株的核心将对所有四个项目都是一个巨大的优势。此外，创新的 过度表达野生型或突变型Panx1的转基因小鼠模型将有助于回答关键问题 在四个主要项目中以额外独特的方式摆出姿势。总的来说，这些小鼠品系/模型和分析 所产生的工具有望在推动我们的人类疾病工作方面发挥根本性的重要作用，并将 也帮助更多的研究人员研究与许多人类有关的PAnnexin通道功能 疾病。

CORE B PROJECT SUMMARY The primary goals of this Program Project application are to understand the function of pannexin channels in normal physiology, as well as well as under conditions of cardiovascular and inflammatory diseases. This Mouse Support Core/Core B will serve all projects in the following ways: (1) This core will provide the husbandry, genotyping and basic crossing of conditional Panx1 knockout mice to specific Cre strains; specifically, the Panx1fl/fl mice crossed to at least five different Cre strains that are relevant to studying cardiovascular and inflammatory diseases. (2) This Core will also help generate additional transgenic Panx1 mice for tissue-specific expression of Panx1-WT and variants. (3) The Core will generate Cre-mediated tissue-speficic knockouts of the Panx1-regulatory proteins Sik1 and LKB1. The key benefits of this Mouse Support Core are that it will help consolidate the breeding and mouse maintenance, save time and cost, and avoid variations due to colonies individually maintained by the four project leaders. As the previous funding period has shown, having a dedicated core to cross the various Cre strains will be a huge advantage to all four projects. Moreover, the innovative transgenic mouse models overexpressing wild type or mutant forms of Panx1 will help answer key questions posed in the four main Projects in additional unique ways. Collectively, these mouse strains/models and analyses tools generated are expected to be fundamentally important in advancing our work to human disease, and will also help the larger community of researchers studying pannexin channel function pertaining to many human diseases.