Mouse Support Core

鼠标支撑核心

• 批准号: 10625321
• 负责人: Ulrike Lorenz
• 金额: $ 39.77万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10625321
• 关键词: Address; Animal Model; Animals; Blood Vessels; Breeding; Cardiovascular Diseases; Cells; Communities; Databases; Dedications; Disease; Ensure; Equipment and supply inventories; Funding; Genes; Genotype; Goals; Individual; Inflammation; Inflammatory; Knock-out; Knockout Mice; LoxP-flanked allele; Maintenance; Mediating; Mission; Modeling; Mouse Strains; Mus; Mutation; Physiology; Proteins; Research Personnel; Resources; STK11 gene; Site; Source; Support System; Time; Tissues; Transgenes; Transgenic Mice; Transgenic Organisms; Variant; Work; base; cost; genetic regulatory protein; human disease; innovation; mouse model; mutant; overexpression; programs; success; tool; transgene expression

CORE B PROJECT SUMMARY The primary goals of this Program Project application are to understand the function of pannexin channels in normal physiology, as well as well as under conditions of cardiovascular and inflammatory diseases. This Mouse Support Core/Core B will serve all projects in the following ways: (1) This core will provide the husbandry, genotyping and basic crossing of conditional Panx1 knockout mice to specific Cre strains; specifically, the Panx1fl/fl mice crossed to at least five different Cre strains that are relevant to studying cardiovascular and inflammatory diseases. (2) This Core will also help generate additional transgenic Panx1 mice for tissue-specific expression of Panx1-WT and variants. (3) The Core will generate Cre-mediated tissue-speficic knockouts of the Panx1-regulatory proteins Sik1 and LKB1. The key benefits of this Mouse Support Core are that it will help consolidate the breeding and mouse maintenance, save time and cost, and avoid variations due to colonies individually maintained by the four project leaders. As the previous funding period has shown, having a dedicated core to cross the various Cre strains will be a huge advantage to all four projects. Moreover, the innovative transgenic mouse models overexpressing wild type or mutant forms of Panx1 will help answer key questions posed in the four main Projects in additional unique ways. Collectively, these mouse strains/models and analyses tools generated are expected to be fundamentally important in advancing our work to human disease, and will also help the larger community of researchers studying pannexin channel function pertaining to many human diseases.

核心B项目总结 本计划项目申请的主要目标是了解泛连接蛋白通道的功能， 正常生理学以及在心血管和炎性疾病的条件下。这个鼠标 支持核心/核心B将以下列方式为所有项目服务：（1）该核心将提供饲养， 条件性Panx 1敲除小鼠与特定Cre品系的基因分型和基础杂交;具体而言， Panx 1fl/fl小鼠与至少五种不同的Cre品系杂交，这些品系与研究心血管和 炎症性疾病。(2)该核心还将有助于产生额外的转基因Panx 1小鼠，用于组织特异性 Panx 1-WT和变体的表达。(3)核心将产生Cre介导的组织特异性敲除， Panx 1-调节蛋白Sik 1和LKB 1。这个鼠标支持核心的主要好处是，它将有助于 统一育种和小鼠维护，节省时间和成本，避免因群体而产生的变异 由四个项目负责人单独维护。正如上一个供资期间所显示的，有一个专门的 核心交叉各种Cre菌株将是一个巨大的优势，所有四个项目。此外，创新 过度表达野生型或突变型Panx 1的转基因小鼠模型将有助于回答关键问题 在四个主要项目中以其他独特的方式提出。总之，这些小鼠品系/模型和分析 所产生的工具预计将在推进我们对人类疾病的研究方面发挥根本性的重要作用，并将 这也有助于研究泛连接蛋白通道功能的更大社区的研究人员， 疾病