Mouse Support Core
鼠标支撑核心
基本信息
- 批准号:10625321
- 负责人:
- 金额:$ 39.77万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-08-01 至 2024-05-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAnimal ModelAnimalsBlood VesselsBreedingCardiovascular DiseasesCellsCommunitiesDatabasesDedicationsDiseaseEnsureEquipment and supply inventoriesFundingGenesGenotypeGoalsIndividualInflammationInflammatoryKnock-outKnockout MiceLoxP-flanked alleleMaintenanceMediatingMissionModelingMouse StrainsMusMutationPhysiologyProteinsResearch PersonnelResourcesSTK11 geneSiteSourceSupport SystemTimeTissuesTransgenesTransgenic MiceTransgenic OrganismsVariantWorkbasecostgenetic regulatory proteinhuman diseaseinnovationmouse modelmutantoverexpressionprogramssuccesstooltransgene expression
项目摘要
CORE B PROJECT SUMMARY
The primary goals of this Program Project application are to understand the function of pannexin channels in
normal physiology, as well as well as under conditions of cardiovascular and inflammatory diseases. This Mouse
Support Core/Core B will serve all projects in the following ways: (1) This core will provide the husbandry,
genotyping and basic crossing of conditional Panx1 knockout mice to specific Cre strains; specifically, the
Panx1fl/fl mice crossed to at least five different Cre strains that are relevant to studying cardiovascular and
inflammatory diseases. (2) This Core will also help generate additional transgenic Panx1 mice for tissue-specific
expression of Panx1-WT and variants. (3) The Core will generate Cre-mediated tissue-speficic knockouts of the
Panx1-regulatory proteins Sik1 and LKB1. The key benefits of this Mouse Support Core are that it will help
consolidate the breeding and mouse maintenance, save time and cost, and avoid variations due to colonies
individually maintained by the four project leaders. As the previous funding period has shown, having a dedicated
core to cross the various Cre strains will be a huge advantage to all four projects. Moreover, the innovative
transgenic mouse models overexpressing wild type or mutant forms of Panx1 will help answer key questions
posed in the four main Projects in additional unique ways. Collectively, these mouse strains/models and analyses
tools generated are expected to be fundamentally important in advancing our work to human disease, and will
also help the larger community of researchers studying pannexin channel function pertaining to many human
diseases.
核心B项目总结
本计划项目申请的主要目标是了解泛连接蛋白通道的功能,
正常生理学以及在心血管和炎性疾病的条件下。这个鼠标
支持核心/核心B将以下列方式为所有项目服务:(1)该核心将提供饲养,
条件性Panx 1敲除小鼠与特定Cre品系的基因分型和基础杂交;具体而言,
Panx 1fl/fl小鼠与至少五种不同的Cre品系杂交,这些品系与研究心血管和
炎症性疾病。(2)该核心还将有助于产生额外的转基因Panx 1小鼠,用于组织特异性
Panx 1-WT和变体的表达。(3)核心将产生Cre介导的组织特异性敲除,
Panx 1-调节蛋白Sik 1和LKB 1。这个鼠标支持核心的主要好处是,它将有助于
统一育种和小鼠维护,节省时间和成本,避免因群体而产生的变异
由四个项目负责人单独维护。正如上一个供资期间所显示的,有一个专门的
核心交叉各种Cre菌株将是一个巨大的优势,所有四个项目。此外,创新
过度表达野生型或突变型Panx 1的转基因小鼠模型将有助于回答关键问题
在四个主要项目中以其他独特的方式提出。总之,这些小鼠品系/模型和分析
所产生的工具预计将在推进我们对人类疾病的研究方面发挥根本性的重要作用,并将
这也有助于研究泛连接蛋白通道功能的更大社区的研究人员,
疾病
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Ulrike Lorenz其他文献
Ulrike Lorenz的其他文献
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{{ truncateString('Ulrike Lorenz', 18)}}的其他基金
A New Approach to Modulating CAR T Cell Activity
调节 CAR T 细胞活性的新方法
- 批准号:
10709301 - 财政年份:2022
- 资助金额:
$ 39.77万 - 项目类别:
A New Approach to Modulating CAR T Cell Activity
调节 CAR T 细胞活性的新方法
- 批准号:
10365202 - 财政年份:2022
- 资助金额:
$ 39.77万 - 项目类别:
Role of SHP-1 in T cell activation and development
SHP-1 在 T 细胞激活和发育中的作用
- 批准号:
7922994 - 财政年份:2009
- 资助金额:
$ 39.77万 - 项目类别:
Role of Bai1 and Elmo Proteins in Apoptotic Cell Clearance
Bai1 和 Elmo 蛋白在凋亡细胞清除中的作用
- 批准号:
8896807 - 财政年份:2003
- 资助金额:
$ 39.77万 - 项目类别:
Role of Bai1 and Elmo Proteins in Apoptotic Cell Clearance
Bai1 和 Elmo 蛋白在凋亡细胞清除中的作用
- 批准号:
8599030 - 财政年份:2003
- 资助金额:
$ 39.77万 - 项目类别:
Role of Bai1 and Elmo Proteins in Apoptotic Cell Clearance
Bai1 和 Elmo 蛋白在凋亡细胞清除中的作用
- 批准号:
8707468 - 财政年份:2003
- 资助金额:
$ 39.77万 - 项目类别:
Role of SHP-1 in T cell activation and development
SHP-1 在 T 细胞激活和发育中的作用
- 批准号:
6749457 - 财政年份:2001
- 资助金额:
$ 39.77万 - 项目类别:
Role of SHP-1 in T cell activation and development
SHP-1 在 T 细胞激活和发育中的作用
- 批准号:
7637435 - 财政年份:2001
- 资助金额:
$ 39.77万 - 项目类别:
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