Acquisition of a Luxendo MuVi Light Sheet Microscope for the University of Rochester Light Microscopy Shared Resource Laboratories

为罗彻斯特大学光学显微镜共享资源实验室购买 Luxendo MuVi 光片显微镜

• 批准号: 10412265
• 负责人: Verda Kaye Thomas
• 金额: $ 59.26万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10412265
• 关键词: 3-Dimensional; Adopted; Biological; CAR T cell therapy; COVID-19; Cellular Structures; Characteristics; Communities; Equipment; Funding; Health; Image; Image Enhancement; Imaging Device; Influenza; Laboratories; Light; Medical center; Microscope; Microscopy; Nanoscopy; New York; Research; Research Activity; Research Personnel; Resolution; Resource Sharing; Resources; Sampling; Scientist; Specimen; Spinal cord injury; Study models; Techniques; Three-Dimensional Imaging; Tissues; Universities; autism spectrum disorder; computing resources; data resource; experience; fluorescence imaging; human disease; imaging capabilities; imaging facilities; imaging system; improved; interest; light microscopy; programs; rapid growth; tool

ABSTRACT This proposal seeks to obtain a Luxendo MuVi Light Sheet Microscope (LSM) with an environmental control unit to be housed in the Light Microscopy Shared Resource Labs (SRLs) of the University of Rochester Medical Center (URMC) as a shared equipment jointly supported by the Multiphoton and Analytical Imaging Center (MAGIC) and the Center for Advanced Light Microscopy and Nanoscopy (CALMN). This microscope would greatly expand and enhance the imaging capabilities of both imaging centers to fulfill the increasing needs of fast three- dimensional imaging of delicate live specimens as well as large cleared tissues from the diverse user groups. Nowadays, researchers are interested in a more complete picture of their studied models with higher degrees of multiplexing and throughput, while our current imaging systems are insufficient to fully capture the three-dimensional characteristics of samples in fast and gentle manners. Bringing in the LSM technique will significantly benefit many current health-related research programs at URMC, which include studies on Influenza, CAR-T therapy, COVID-19, Spinal Cord Injury, Autism etc., by significantly improving the spatial and temporal imaging capabilities. The technological breakthroughs using light sheet microscopy will advance those health-related research with better understandings of the intricate dynamics and structures of cells within living and intact specimens. High resolution fluorescence imaging has proven an invaluable and central tool for the advancement of biomedical discoveries. The URMC Light Microscopy SRLs have seen a rapid growth in the last three years with the join of two experienced directors introducing three new cutting-edge imaging instruments that have greatly enhanced the community’s funded research activities. Acquisition of a light sheet microscope will add new impetus to establishing a state-of- the-art comprehensive imaging resource at the URMC and the upstate New York that will continuously provide scientists new avenues for bearing on fundamental questions pertaining to human diseases. Additionally, by leveraging the local data and computational resources, the Light Microscopy SRLs will maximize the impact of the light sheet microscopy technique that can be widely adopted by various research programs to further promote and advance biological discoveries.

摘要