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500 MHz NMR Spectrometer Upgrade and Cryoprobe

500 MHz NMR 波谱仪升级和冷冻探针

基本信息

批准号：
10412824
负责人：
Nicholas Fraser Breen
金额：
$ 60万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-04-01 至 2023-03-31
项目状态：
已结题

项目摘要

PROJECT SUMMARY/ABSTRACT We request the funding to purchase a Bruker NEO 500 MHz NMR spectrometer, equipped with a Prodigy (nitrogen boiloff) cryoprobe and a SampleCase 24-position sample changer. The existing NMR capabilities at the University of Washington, Seattle (UW) are increasingly both obsolete and unreliable, and indeed we recently had to decommission one instrument where neither the electronics nor magnet could be economically repaired. The technical limitations of much of our remaining instrumentation are slowing or blocking many lines of investigation on NIH-funded research in multiple departments. The proposed system will simultaneously improve reliability and efficiency by replacing our fastest-failing instrument and expand our research capabilities with advances in NMR technology that our decades-old equipment cannot provide. These include: (1) drastically increased sensitivity, 2-3 fold, across a range of nuclei ranging from 15N to 19F – an enhancement particularly valuable for 31P and 19F, nuclei of wide- ranging research utility that are badly underserved with existing equipment, and for which this will become the most sensitive instrument available on campus by a wide margin; (2) a combination of an automatic sample changer, automatic probe tuning and shimming, and automation software, which collectively provide users with highly efficient, hands-off sample handling to supply continued, productive instrument use during unattended hours; and (3) dynamic, wide-ranged temperature control, with experimental variability over a broad range to study reaction dynamics, and 0.1°C-precision stability to hold delicate biochemical samples at a safe, consistent temperature during storage and data acquisition. From small molecules to large proteins, NMR is a vital tool for determining structure, kinetics, and molecular interactions, relied upon by dozens of research groups on campus. This instrument will supply crucial support to full range of projects related to human health, including the development of new biomedical screening technologies, new synthetic pathways for pharmaceutical developments, mechanistic investigations of enzymatic tumor suppressors, metabolomic nutritional studies bolstering the recovery of critically ill patients, and many more.

项目总结/摘要我们申请资金购买布鲁克NEO 500 MHz NMR光谱仪，配备Prodigy （氮气蒸发）冷冻探针和SampleCase 24位样品更换器。现有的NMR 位于西雅图的华盛顿大学（UW）的能力越来越过时和不可靠，事实上，我们最近不得不退役一个仪器，可以经济地修复。我们剩下的仪器的技术局限性是减缓或阻止了对NIH资助的多个部门的研究的许多调查。的拟议的系统将同时提高可靠性和效率，取代我们最快的失败，仪器和扩大我们的研究能力与核磁共振技术的进步，我们几十年的历史设备无法提供。这些措施包括：（1）在一定范围内，灵敏度大幅提高2-3倍，核的范围从15 N到19 F-一个增强特别有价值的31 P和19 F，核的宽- 现有设备严重不足的测距研究实用程序，这将成为校园里最灵敏的仪器;（2）自动自动探头调谐和匀场以及自动化软件，为用户提供高效、无需手动的样品处理，以提供持续、高效的仪器在无人看管的时间内使用;和（3）动态，宽范围的温度控制，实验广泛的可变性，以研究反应动力学，0.1° C精度的稳定性，以保持微妙的在储存和数据采集过程中，生物化学样品处于安全、一致的温度。从小从大分子到大蛋白质，NMR是确定结构，动力学和分子结构的重要工具。互动，依赖于几十个研究小组在校园里。该仪器将提供至关重要的支持与人类健康相关的全方位项目，包括开发新的生物医学筛选技术，用于药物开发的新合成途径，研究酶促肿瘤抑制剂，代谢组学营养研究支持恢复重症患者，还有更多。