500 MHz NMR Spectrometer Upgrade and Cryoprobe

项目摘要/摘要 我们请求资金购买一台布鲁克NEO 500 MHz核磁共振光谱仪，配备一台Prodigy (氮气蒸发)冷冻探头和SampleCase 24位换样器。现有的核磁共振 华盛顿大学西雅图分校(UW)的能力越来越过时和不可靠， 事实上，我们最近不得不退役一台仪器，在那里，电子设备和磁铁都没有 可以在经济上得到修复。我们剩余的许多工具的技术限制是 减缓或阻止多个部门对NIH资助的研究的多条调查路线。这个 建议的系统将通过替换我们故障最快的系统来同时提高可靠性和效率 用我们几十年来的核磁共振技术的进步来检测和扩展我们的研究能力 设备无法提供。这些包括：(1)敏感度大幅提高，在一定范围内提高2-3倍 从15N到19F的原子核-这一增强对31P和19F特别有价值，宽的原子核- 现有设备严重不足的范围研究实用程序，这将成为 校园内最灵敏的仪器遥遥领先；(2)自动 样品转换器、自动探头调谐和垫片以及自动化软件，这些共同构成了 为用户提供高效、不插手的样品处理，以提供持续、高效的仪器 在无人值守的时间内使用；以及(3)动态、宽范围的温度控制，具有试验性 用于研究反应动力学的大范围可变性，以及0.1°C的精确稳定性以保持微妙 生化样品在储存和数据采集过程中保持安全、一致的温度。从小到大 从分子到大蛋白质，核磁共振是确定结构、动力学和分子的重要工具 互动，由校园里的数十个研究小组依赖。这台仪器将提供至关重要的 支持与人类健康有关的各种项目，包括开发新的生物医学 筛选技术，药物开发的新合成途径，机械学 酶促肿瘤抑制物的研究，代谢营养研究支持康复 危重病人，还有更多。

PROJECT SUMMARY/ABSTRACT We request the funding to purchase a Bruker NEO 500 MHz NMR spectrometer, equipped with a Prodigy (nitrogen boiloff) cryoprobe and a SampleCase 24-position sample changer. The existing NMR capabilities at the University of Washington, Seattle (UW) are increasingly both obsolete and unreliable, and indeed we recently had to decommission one instrument where neither the electronics nor magnet could be economically repaired. The technical limitations of much of our remaining instrumentation are slowing or blocking many lines of investigation on NIH-funded research in multiple departments. The proposed system will simultaneously improve reliability and efficiency by replacing our fastest-failing instrument and expand our research capabilities with advances in NMR technology that our decades-old equipment cannot provide. These include: (1) drastically increased sensitivity, 2-3 fold, across a range of nuclei ranging from 15N to 19F – an enhancement particularly valuable for 31P and 19F, nuclei of wide- ranging research utility that are badly underserved with existing equipment, and for which this will become the most sensitive instrument available on campus by a wide margin; (2) a combination of an automatic sample changer, automatic probe tuning and shimming, and automation software, which collectively provide users with highly efficient, hands-off sample handling to supply continued, productive instrument use during unattended hours; and (3) dynamic, wide-ranged temperature control, with experimental variability over a broad range to study reaction dynamics, and 0.1°C-precision stability to hold delicate biochemical samples at a safe, consistent temperature during storage and data acquisition. From small molecules to large proteins, NMR is a vital tool for determining structure, kinetics, and molecular interactions, relied upon by dozens of research groups on campus. This instrument will supply crucial support to full range of projects related to human health, including the development of new biomedical screening technologies, new synthetic pathways for pharmaceutical developments, mechanistic investigations of enzymatic tumor suppressors, metabolomic nutritional studies bolstering the recovery of critically ill patients, and many more.