BSL3 Flow Sorter for Human Pathogens of Global Significance

项目概要： 该提案要求为4激光，12色Sony MA 900细胞分选机提供资金，内置II级生物防护 罩和分选沉积系统。该仪器将被安置在Cornell的动物BSL 3套间中 兽医学院东校区研究设施作为学院支持的BSL 3流程的一部分 和基因组学机构该工具旨在支持已建立的BSL 3研究活动， 包括结核分枝杆菌、HIV-1和流感的项目， 在SARS-CoV-2中。仪器在ABSL 3中的位置旨在最好地支持分类 体内动物模型感染的感染细胞。该工具将建设东道国的研究能力， 病原体相互作用，以响应最近国家和国际对BSL 3研究增加的需求 专业知识和能力。我们选择了索尼MA 900细胞分选机，因为它相对易于使用， 模块化结构，包括可更换的射流系统，以及我们在该仪器方面的积极经验 模型在康奈尔大学BRC流式细胞仪核心在伊萨卡校园。 在感染生物学程序中添加流分类功能可以是革命性的， 病原体、宿主细胞和免疫效应物的遗传和表观遗传分析 细胞，以及分选细胞群用于下游功能测定。其中两个（DGR和BCV） 我们已经从BSL 3计划中纳入的流程分选能力中受益，并发现 技术是变革性的。大卫博士G.罗素（PD/PI），NIH资助的感染生物学家， BSL 3经验，建议建立一个新的BSL 3流动和基因组学设施，提供细胞分选能力， 10倍的基因组文库生成能力，以BSL 3研究计划在康奈尔大学。用户组 （7个主要用户和1个次要用户）包括NIH资助的具有免疫学、病毒学、 细菌学、药物发现以及宿主和病原体遗传学的不同方面。一个非国家卫生研究院 Diego Diel博士是一名受资助的研究人员，他得到了美国农业部的大力支持，并致力于SARS-CoV-2的动物模型研究。 我们预计，在校园内建立这种能力将为学员提供机会，促进 合作和创新，促进与NIH目标相关的新研究的发展，并增加 康奈尔大学作为宿主-病原体相互作用研究中心的概况， 全球健康的挑战。

Project Summary: This proposal requests funding for a 4 laser, 12 color Sony MA900 Cell sorter with built-in Class II Biocontainment Hood and a Sorting Deposition System. The instrument will be housed in the Animal BSL3 Suite in the Cornell East Campus Research Facility at the College of Veterinary Medicine as part of a College-supported BSL3 Flow and Genomics Facility. The instrument is intended to support both established BSL3 research activities, including programs on Mycobacterium tuberculosis, HIV-1 and influenza, in addition to emerging research programs in SARS-CoV-2. The location of the instrument in the ABSL3 is intended to best support the sorting of infected cells from in vivo animal model infections. The instrument will build capacity in research on host- pathogen interactions in response to recent national and international needs for increased BSL3 research expertise and capabilities. We have chosen the Sony MA900 Cell Sorter because of its relative ease of use, its modular construction, including exchangeable fluidics systems, and our positive experience with this instrument model in the Cornell BRC Flow Cytometry Core on the Ithaca campus. The addition of flow sorting capabilities to an infection biology program can be revolutionary and facilitate genetic and chemical screens, genetic and epigenetic profiling of pathogens, host cells and immune effector cells, and the sorting of cell populations for downstream functional assays. Two of us (DGR and BCV) have already benefited from incorporation of flow sorting capacity into our BSL3 programs and have found the technology to be transformatory. Dr. David G. Russell (PD/PI), an NIH-funded infection biologist with extensive BSL3 experience, proposes building a new BSL3 Flow and Genomics Facility providing cell sorting capacity and 10X genomics library generation capabilities to BSL3 research programs at Cornell University. The User group (7 Major and 1 Minor Users) includes NIH-funded investigators with expertise in immunology, virology, bacteriology, drug discovery and different aspects of the genetics of host and pathogen. The one non-NIH funded investigator, Dr. Diego Diel, is heavily USDA-supported and works on animal models for SARS-CoV-2. We anticipate that the establishment of this capacity on campus will provide opportunities for trainees, promote collaboration and innovation, foster development of new research relevant to the goals of the NIH, and increase the profile of Cornell University as a center for research in host-pathogen interactions of significance to current challenges to Global Health.