BSL3 Flow Sorter for Human Pathogens of Global Significance

BSL3 流式分选机用于检测具有全球意义的人类病原体

• 批准号: 10412511
• 负责人: DAVID G RUSSELL
• 金额: $ 36.23万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-03-01 至 2023-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10412511
• 关键词: 2019-nCoV; Animal Model; Animals; Bacteriology; Biological Assay; Biology; Cell Separation; Cells; Chemicals; Collaborations; Color; Deposition; Development; Effector Cell; Flow Cytometry; Fostering; Funding; Generations; Genetic; Genomic Library; Genomics; Goals; HIV-1; Immune; Immunology; Infection; Influenza; International; Lasers; Liquid substance; Location; Minor; Modeling; Mycobacterium tuberculosis; Population; Request for Proposals; Research; Research Activity; Research Personnel; Sorting - Cell Movement; System; Technology; United States Department of Agriculture; United States National Institutes of Health; Universities; Veterinary Medicine; Work; college; drug discovery; epigenetic profiling; experience; genetic profiling; global health; human pathogen; in vivo; innovation; instrument; pathogen; programs; research facility; response; virology

Project Summary: This proposal requests funding for a 4 laser, 12 color Sony MA900 Cell sorter with built-in Class II Biocontainment Hood and a Sorting Deposition System. The instrument will be housed in the Animal BSL3 Suite in the Cornell East Campus Research Facility at the College of Veterinary Medicine as part of a College-supported BSL3 Flow and Genomics Facility. The instrument is intended to support both established BSL3 research activities, including programs on Mycobacterium tuberculosis, HIV-1 and influenza, in addition to emerging research programs in SARS-CoV-2. The location of the instrument in the ABSL3 is intended to best support the sorting of infected cells from in vivo animal model infections. The instrument will build capacity in research on host- pathogen interactions in response to recent national and international needs for increased BSL3 research expertise and capabilities. We have chosen the Sony MA900 Cell Sorter because of its relative ease of use, its modular construction, including exchangeable fluidics systems, and our positive experience with this instrument model in the Cornell BRC Flow Cytometry Core on the Ithaca campus. The addition of flow sorting capabilities to an infection biology program can be revolutionary and facilitate genetic and chemical screens, genetic and epigenetic profiling of pathogens, host cells and immune effector cells, and the sorting of cell populations for downstream functional assays. Two of us (DGR and BCV) have already benefited from incorporation of flow sorting capacity into our BSL3 programs and have found the technology to be transformatory. Dr. David G. Russell (PD/PI), an NIH-funded infection biologist with extensive BSL3 experience, proposes building a new BSL3 Flow and Genomics Facility providing cell sorting capacity and 10X genomics library generation capabilities to BSL3 research programs at Cornell University. The User group (7 Major and 1 Minor Users) includes NIH-funded investigators with expertise in immunology, virology, bacteriology, drug discovery and different aspects of the genetics of host and pathogen. The one non-NIH funded investigator, Dr. Diego Diel, is heavily USDA-supported and works on animal models for SARS-CoV-2. We anticipate that the establishment of this capacity on campus will provide opportunities for trainees, promote collaboration and innovation, foster development of new research relevant to the goals of the NIH, and increase the profile of Cornell University as a center for research in host-pathogen interactions of significance to current challenges to Global Health.

项目摘要： 该提案要求提供4个激光，12彩色的Sony MA900手机分隔器，并带有II类生物内在疗法 引擎盖和分类沉积系统。该乐器将安置在康奈尔的动物BSL3套件中 作为大学支持的BSL3流量的一部分，兽医学院的东校园研究机构 和基因组学设施。该工具旨在支持两项已建立的BSL3研究活动， 包括结核分枝杆菌，HIV-1和流感的程序，除了新兴研究 SARS-COV-2中的程序。仪器在ABSL3中的位置旨在最好地支持分类 体内动物模型感染的感染细胞。该工具将在主机研究中建立能力 - 病原体相互作用响应最近的国家和国际需求增加BSL3研究 专业知识和能力。我们之所以选择索尼MA900单元格分子，是因为它相对易于使用， 模块化结构，包括可交换的流体系统，以及我们在该乐器上的积极经验 在伊萨卡校园的康奈尔BRC流式细胞仪核心中的模型。 在感染生物学计划中添加流动分选功能可能是革命性的，有助于 遗传和化学筛选，病原体，宿主细胞和免疫效应子的遗传和表观遗传分析 细胞，以及用于下游功能测定的细胞群体的分类。我们两个人（DGR和BCV） 已经从将流动能力纳入我们的BSL3计划中已经受益，并发现 技术要转化。 David G. Russell博士（PD/PI），一位由NIH资助的感染生物学家，广泛 BSL3经验，提议建立一个新的BSL3流量和基因组学设施，以提供细胞分类能力和 康奈尔大学BSL3研究计划的10倍基因组图书馆生成能力。用户组 （7名主要和1个未成年人）包括拥有免疫学，病毒学专业知识的NIH资助的研究人员 细菌学，药物发现以及宿主和病原体遗传学的不同方面。一个非NIH 资助的研究员迭戈·迪尔（Diego Diel）博士受到了美国农业部支持的大量支持，并为SARS-COV-2的动物模型工作。 我们预计，在校园内建立这种能力将为受训者提供机会，促进 协作与创新，促进与NIH目标相关的新研究的发展，并增加 康奈尔大学作为宿主 - 病原体相互作用研究中心的特征 全球健康的挑战。