BSL3 Flow Sorter for Human Pathogens of Global Significance
BSL3 流式分选机用于检测具有全球意义的人类病原体
基本信息
- 批准号:10412511
- 负责人:
- 金额:$ 36.23万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-03-01 至 2023-02-28
- 项目状态:已结题
- 来源:
- 关键词:2019-nCoVAnimal ModelAnimalsBacteriologyBiological AssayBiologyCell SeparationCellsChemicalsCollaborationsColorDepositionDevelopmentEffector CellFlow CytometryFosteringFundingGenerationsGeneticGenomic LibraryGenomicsGoalsHIV-1ImmuneImmunologyInfectionInfluenzaInternationalLasersLiquid substanceLocationMinorModelingMycobacterium tuberculosisPopulationRequest for ProposalsResearchResearch ActivityResearch PersonnelSorting - Cell MovementSystemTechnologyUnited States Department of AgricultureUnited States National Institutes of HealthUniversitiesVeterinary MedicineWorkcollegedrug discoveryepigenetic profilingexperiencegenetic profilingglobal healthhuman pathogenin vivoinnovationinstrumentpathogenprogramsresearch facilityresponsevirology
项目摘要
Project Summary:
This proposal requests funding for a 4 laser, 12 color Sony MA900 Cell sorter with built-in Class II Biocontainment
Hood and a Sorting Deposition System. The instrument will be housed in the Animal BSL3 Suite in the Cornell
East Campus Research Facility at the College of Veterinary Medicine as part of a College-supported BSL3 Flow
and Genomics Facility. The instrument is intended to support both established BSL3 research activities,
including programs on Mycobacterium tuberculosis, HIV-1 and influenza, in addition to emerging research
programs in SARS-CoV-2. The location of the instrument in the ABSL3 is intended to best support the sorting
of infected cells from in vivo animal model infections. The instrument will build capacity in research on host-
pathogen interactions in response to recent national and international needs for increased BSL3 research
expertise and capabilities. We have chosen the Sony MA900 Cell Sorter because of its relative ease of use, its
modular construction, including exchangeable fluidics systems, and our positive experience with this instrument
model in the Cornell BRC Flow Cytometry Core on the Ithaca campus.
The addition of flow sorting capabilities to an infection biology program can be revolutionary and facilitate
genetic and chemical screens, genetic and epigenetic profiling of pathogens, host cells and immune effector
cells, and the sorting of cell populations for downstream functional assays. Two of us (DGR and BCV) have
already benefited from incorporation of flow sorting capacity into our BSL3 programs and have found the
technology to be transformatory. Dr. David G. Russell (PD/PI), an NIH-funded infection biologist with extensive
BSL3 experience, proposes building a new BSL3 Flow and Genomics Facility providing cell sorting capacity and
10X genomics library generation capabilities to BSL3 research programs at Cornell University. The User group
(7 Major and 1 Minor Users) includes NIH-funded investigators with expertise in immunology, virology,
bacteriology, drug discovery and different aspects of the genetics of host and pathogen. The one non-NIH
funded investigator, Dr. Diego Diel, is heavily USDA-supported and works on animal models for SARS-CoV-2.
We anticipate that the establishment of this capacity on campus will provide opportunities for trainees, promote
collaboration and innovation, foster development of new research relevant to the goals of the NIH, and increase
the profile of Cornell University as a center for research in host-pathogen interactions of significance to current
challenges to Global Health.
项目总结:
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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DAVID G RUSSELL其他文献
DAVID G RUSSELL的其他文献
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{{ truncateString('DAVID G RUSSELL', 18)}}的其他基金
Modulation of epigenetic programming of tissue resident macrophage lineages to impact HIV-1 infection, maintenance, and persistence.
调节组织驻留巨噬细胞谱系的表观遗传编程以影响 HIV-1 感染、维持和持久性。
- 批准号:
10675934 - 财政年份:2023
- 资助金额:
$ 36.23万 - 项目类别:
Minimizing in vivo Drug Tolerance induction in tuberculosis.
最大限度地减少结核病体内药物耐受性的诱导。
- 批准号:
10665033 - 财政年份:2021
- 资助金额:
$ 36.23万 - 项目类别:
Minimizing in vivo Drug Tolerance induction in tuberculosis.
最大限度地减少结核病体内药物耐受性的诱导。
- 批准号:
10271650 - 财政年份:2021
- 资助金额:
$ 36.23万 - 项目类别:
Minimizing in vivo Drug Tolerance induction in tuberculosis.
最大限度地减少结核病体内药物耐受性的诱导。
- 批准号:
10493281 - 财政年份:2021
- 资助金额:
$ 36.23万 - 项目类别:
Are HIV-1-Infected Alveolar Macrophages Productive Sites of Viral Persistence?
HIV-1 感染的肺泡巨噬细胞是病毒持续存在的产生场所吗?
- 批准号:
10452659 - 财政年份:2018
- 资助金额:
$ 36.23万 - 项目类别:
Are HIV-1-Infected Alveolar Macrophages Productive Sites of Viral Persistence?
HIV-1 感染的肺泡巨噬细胞是病毒持续存在的产生场所吗?
- 批准号:
10224994 - 财政年份:2018
- 资助金额:
$ 36.23万 - 项目类别:
Are HIV-1-Infected Alveolar Macrophages Productive Sites of Viral Persistence?
HIV-1 感染的肺泡巨噬细胞是病毒持续存在的产生场所吗?
- 批准号:
10240741 - 财政年份:2018
- 资助金额:
$ 36.23万 - 项目类别:
A mechanistic understanding of tuberculosis progression through bacterial reporter strains
通过细菌报告菌株了解结核病进展的机制
- 批准号:
10217964 - 财政年份:2017
- 资助金额:
$ 36.23万 - 项目类别:
A mechanistic understanding of tuberculosis progression through bacterial reporter strains
通过细菌报告菌株了解结核病进展的机制
- 批准号:
9409031 - 财政年份:2017
- 资助金额:
$ 36.23万 - 项目类别:
Do HIV-infected Alveolar Macrophages represent a cART-resistant Reservoir?
HIV 感染的肺泡巨噬细胞是否代表 cART 耐药性储库?
- 批准号:
9306347 - 财政年份:2016
- 资助金额:
$ 36.23万 - 项目类别:
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