BSL3 Flow Sorter for Human Pathogens of Global Significance

项目总结： 这项提案要求提供资金，用于4激光、12色索尼MA900细胞分选机，该分选机具有内置的II类生物遏制装置 引擎盖和分类取证系统。该乐器将被安置在康奈尔大学的动物BSL3套房中 作为学院支持的BSL3流的一部分，兽医学院的东校园研究设施 和基因组学设施。该工具的目的是支持已建立的《生物多样性公约》3研究活动， 包括关于结核分枝杆菌、艾滋病毒-1和流感的项目，以及新出现的研究 SARS-CoV-2中的计划。仪器在ABSL3中的位置旨在最好地支持分类 来自体内动物模型感染的受感染细胞。该仪器将在宿主研究方面建立能力- 病原体相互作用对最近国内和国际上增加BSL3研究的需求的响应 专业知识和能力。我们选择了索尼MA900细胞分选器，因为它相对容易使用，它的 模块化结构，包括可交换的流体系统，以及我们使用该仪器的积极经验 在伊萨卡校区的康奈尔BRC流式细胞仪核心中的模型。 将流分类功能添加到感染生物学程序可以是革命性和便利的 病原体、宿主细胞和免疫效应物的遗传和化学筛选、遗传和表观遗传学分析 细胞，以及用于下游功能分析的细胞群体分类。我们中的两个(DGR和BCV) 已经受益于将流分类能力纳入我们的BSL3计划，并发现 技术是变革性的。大卫·G·罗素博士(PD/PI)，NIH资助的感染生物学家，具有广泛的 BSL3经验，建议建立新的BSL3 Flow和基因组学设施，提供细胞分选能力和 10倍于康奈尔大学BSL3研究项目的基因组文库生成能力。用户组 (7个主要用户和1个次要用户)包括由NIH资助的具有免疫学、病毒学、 细菌学、药物发现以及宿主和病原体遗传学的不同方面。一位非美国国立卫生研究院 资助的研究员Diego Diel博士得到了美国农业部的大力支持，并致力于SARS-CoV-2的动物模型工作。 我们预计，在校园内建立这一能力将为学员提供机会，促进 合作和创新，促进与国家卫生研究院目标相关的新研究的开发，并增加 康奈尔大学作为宿主-病原体相互作用研究中心的简介 全球卫生面临的挑战。

Project Summary: This proposal requests funding for a 4 laser, 12 color Sony MA900 Cell sorter with built-in Class II Biocontainment Hood and a Sorting Deposition System. The instrument will be housed in the Animal BSL3 Suite in the Cornell East Campus Research Facility at the College of Veterinary Medicine as part of a College-supported BSL3 Flow and Genomics Facility. The instrument is intended to support both established BSL3 research activities, including programs on Mycobacterium tuberculosis, HIV-1 and influenza, in addition to emerging research programs in SARS-CoV-2. The location of the instrument in the ABSL3 is intended to best support the sorting of infected cells from in vivo animal model infections. The instrument will build capacity in research on host- pathogen interactions in response to recent national and international needs for increased BSL3 research expertise and capabilities. We have chosen the Sony MA900 Cell Sorter because of its relative ease of use, its modular construction, including exchangeable fluidics systems, and our positive experience with this instrument model in the Cornell BRC Flow Cytometry Core on the Ithaca campus. The addition of flow sorting capabilities to an infection biology program can be revolutionary and facilitate genetic and chemical screens, genetic and epigenetic profiling of pathogens, host cells and immune effector cells, and the sorting of cell populations for downstream functional assays. Two of us (DGR and BCV) have already benefited from incorporation of flow sorting capacity into our BSL3 programs and have found the technology to be transformatory. Dr. David G. Russell (PD/PI), an NIH-funded infection biologist with extensive BSL3 experience, proposes building a new BSL3 Flow and Genomics Facility providing cell sorting capacity and 10X genomics library generation capabilities to BSL3 research programs at Cornell University. The User group (7 Major and 1 Minor Users) includes NIH-funded investigators with expertise in immunology, virology, bacteriology, drug discovery and different aspects of the genetics of host and pathogen. The one non-NIH funded investigator, Dr. Diego Diel, is heavily USDA-supported and works on animal models for SARS-CoV-2. We anticipate that the establishment of this capacity on campus will provide opportunities for trainees, promote collaboration and innovation, foster development of new research relevant to the goals of the NIH, and increase the profile of Cornell University as a center for research in host-pathogen interactions of significance to current challenges to Global Health.