Developing effective nanovaccines against pathogenic Escherichia coli
开发针对致病性大肠杆菌的有效纳米疫苗
基本信息
- 批准号:10413247
- 负责人:
- 金额:$ 19.75万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-06-01 至 2024-05-31
- 项目状态:已结题
- 来源:
- 关键词:AdherenceAffectAntibodiesAntibody titer measurementAntigensAttentionBacteriaBindingCellsChildCoupledCouplesCouplingDataDevelopmentDiarrheaDiseaseDisease OutbreaksEpithelial CellsEscherichia coliEscherichia coli EHECEscherichia coli InfectionsEscherichia coli O157:H7Fimbrial AdhesinsFoodFormulationGoalsHemolytic-Uremic SyndromeHemorrhagic colitisHumanImmune responseImmunityImmunizationImmunizeInfectionInfection preventionInjectionsIntestinesKnowledgeLesionLinkMediatingMucosal Immune ResponsesMusOutcomePilumPlayPreventiveProcessPropertyProteinsReproducibilityResearchRoleSerotypingSerumShiga ToxinSubunit VaccinesSurfaceTestingToxinType III Secretion System PathwayVaccinatedVaccinesVariantVegetablesVirulenceVirulence FactorsVirulentWorkbasebeefbiothreatcombatcross immunitydiarrheal diseaseenteric infectionenteric pathogenenteroaggregative Escherichia colienteropathogenic Escherichia coliexperimental studyfoodborne pathogengut colonizationimmunogenicimprovedinnovationinsightintestinal epitheliummicrobiotamouse modelmucosal vaccinenanoGoldnanovaccinenovelpathogenpathogenic Escherichia colipathogenic bacteriapreventsoundtissue tropismvaccine development
项目摘要
Abstract
Our scientific contribution to the field of pathogenic Escherichia coli research has been to accelerate the
development of an effective vaccine, using novel antigens and murine models of intestinal infection, that can
not only protect against enterohemorrhagic E. coli (EHEC) O157:H7 but also prevent colonization of other
pathogenic E. coli infections. Therefore, the long-term goal of our study is to provide new fundamental
knowledge regarding pathogenic E. coli colonization, while elucidating protective immune responses that can
be incorporated in the development of a vaccine. The central hypothesis of our proposal is that novel antigens
coupled to nanovaccines will be immunogenic, and stimulation of protective antibodies will disrupt colonization
by different pathogenic E. coli strains. This hypothesis is based on the strong experimental premise
demonstrating that gold nanoparticles coupled to surface-exposed EHEC antigens (defined by our bio-
immunoinformatic approach) elicited host immune responses that correlate with reduction in the intestinal
colonization by EHEC O157:H7. Our progress in recent years demonstrated that coupling antigens to gold
nanoparticles significantly enhances protective immunity against EHEC O157:H7 infection, and the serum from
vaccinated mice reduces adherence/virulence of enteropathogenic E. coli O127:H6 and enteroaggregative E.
coli O104:H4. Our proposed experimental approach will focus on two reproducible and scientifically sound
aims: Optimize a gold nanoparticle vaccine containing antigens that prevent EHEC infections (Aim 1), and
elucidate the immune responses required to prevent colonization and test cross-protective properties against
other pathogenic E. coli strains (Aim 2). This work is significant because it is expected to provide advancement
in the development of a fully protective gold nanoparticle vaccine that prevents infections caused by EHEC
O157:H7, and such a vaccine will also be effective at targeting other pathogenic E. coli strains, without
affecting the commensal E. coli microbiota. This study will elucidate the common links in the virulence
mechanisms of these bacterial pathogens, which can then be used for the development of pathogenic E. coli-
specific mucosal vaccines.
摘要
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Efficacy of EHEC gold nanoparticle vaccines evaluated with the Shiga toxin-producing Citrobacter rodentium mouse model.
- DOI:10.1128/spectrum.02261-23
- 发表时间:2024-01-11
- 期刊:
- 影响因子:3.7
- 作者:
- 通讯作者:
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Alfredo G Torres其他文献
Alfredo G Torres的其他文献
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{{ truncateString('Alfredo G Torres', 18)}}的其他基金
Developing effective nanovaccines against pathogenic Escherichia coli
开发针对致病性大肠杆菌的有效纳米疫苗
- 批准号:
10300897 - 财政年份:2021
- 资助金额:
$ 19.75万 - 项目类别:
Defining the role of toxin-antitoxin systems in persistence of Burkholderia pseudomallei
定义毒素-抗毒素系统在类鼻疽伯克霍尔德杆菌持久性中的作用
- 批准号:
10194359 - 财政年份:2020
- 资助金额:
$ 19.75万 - 项目类别:
Glycoconjugate Nanoparticle Vaccines Against Burkholderia Infections
针对伯克霍尔德氏菌感染的糖复合物纳米颗粒疫苗
- 批准号:
9186787 - 财政年份:2016
- 资助金额:
$ 19.75万 - 项目类别:
Glycoconjugate Nanoparticle Vaccines Against Burkholderia Infections
针对伯克霍尔德氏菌感染的糖复合物纳米颗粒疫苗
- 批准号:
9282736 - 财政年份:2016
- 资助金额:
$ 19.75万 - 项目类别:
Vaccine Development for Burkholderia amllei and B. pseudomallei
鼻疽伯克霍尔德杆菌和类鼻疽伯克霍尔德杆菌的疫苗开发
- 批准号:
8377054 - 财政年份:2012
- 资助金额:
$ 19.75万 - 项目类别:
Long Polar Fimbriae of Attaching and Effacing Escherichia coli
附着和消除大肠杆菌的长极菌毛
- 批准号:
7846683 - 财政年份:2009
- 资助金额:
$ 19.75万 - 项目类别:
Long Polar Fimbriae of Attaching and Effacing Escherichia coli
附着和消除大肠杆菌的长极菌毛
- 批准号:
7795038 - 财政年份:2009
- 资助金额:
$ 19.75万 - 项目类别:
Long Polar Fimbriae of Attaching and Effacing Escherichia coli
附着和消除大肠杆菌的长极菌毛
- 批准号:
7662935 - 财政年份:2009
- 资助金额:
$ 19.75万 - 项目类别:
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