IVIS Spectrum CT imager for the Medical College of Wisconsin

摘要 我们申请资金购买一台新的IVIS Spectrum-CT仪器， CCD摄像机、集成光学和微型CT源和探测器以及3D扩散断层重建 软件该系统包括生物发光和荧光幻影小鼠软件和啮齿动物 麻醉系统该仪器将安装在生物医学成像共享资源（BISR）设施中 在威斯康星州医学院（MCW）。自2004年成立以来，BISR一直支持生物医学 在MCW及其合作机构的NIH资助的研究人员的不同群体的研究计划， 威斯康星州东南部的临床和转化科学研究所（CTSI）。与其记录的主要 在医疗和生物医学成像仪器和设施的投资，MCW已承诺大量 配套资金购买维修合同，提供仪器技术支持。的IVIS Spectrum-CT提供低剂量的2D和3D定量生物发光和多光谱荧光 计算机断层扫描（CT）、光谱分解和Cerenkov成像目前在过时的 MCW现有的光学和微型CT仪器。 获得所要求的完全集成的光学和微型CT成像平台将显著有利于研究 八个联邦资助的项目（NIH，DOD）主要用户调查员和四个次要用户调查员， 在本申请中描述。这些项目将受益于所要求的仪器，因为增加了 分辨率和灵敏度;跨成像模式的空间配准;以及可重复的、非侵入性的和 最小电离检查，使纵向成像跟踪疾病的发病机制和治疗 啮齿动物模型的反应。安装后，该仪器将成为唯一的多模式3D成像仪器 在密尔沃基和威斯康星州东南部进行体内小动物成像。 BISR已经配备了基础设施，并配备了安装所需的支持人员 和IVIS Spectrum-CT的专业操作新仪器将由有经验的人员维护 在操作和维护体内啮齿动物成像仪器、小动物麻醉和管理 多用户充值设施。将使用现有的BISR调度系统分配仪器时间 在iLabs中实现。一个常设的地方咨询委员会将提供监督。BISR的财务运作是 由MCW癌症中心的高级业务管理员管理。IVIS Spectrum-CT的采集 本申请所要求的仪器将对NIH资助的生物医学研究产生深远的影响 MCW及其合作伙伴CTSI机构的课程。

SUMMARY ABSTRACT We request funds to purchase a new IVIS Spectrum-CT instrument equipped with a thermoelectrically-cooled CCD camera, integrated optical and micro-CT sources and detectors, and 3D diffuse tomographic reconstruction software. The system includes bioluminescent and fluorescent phantom mouse software and a rodent anesthesia system. This instrument will be installed in the Biomedical Imaging Shared Resource (BISR) facility at the Medical College of Wisconsin (MCW). Since its creation in 2004, the BISR has supported the biomedical research programs of a diverse group of NIH-funded investigators at MCW and its partner institutions in the Clinical and Translational Science Institute (CTSI) of Southeastern Wisconsin. Consistent with its record of major investments in medical and biomedical imaging instrumentation and facilities, MCW has committed substantial matching funds to purchase the maintenance contract and provide technical support for the instrument. The IVIS Spectrum-CT provides 2D and 3D quantitative bioluminescence and multispectral fluorescence with low dose computed tomography (CT), spectral unmixing, and Cerenkov imaging currently unavailable in the obsolete optical and micro-CT instruments existing at MCW. Access to the requested fully integrated optical and micro-CT imaging platform will markedly benefit the research projects of eight federally funded (NIH, DOD) Major User investigators and four Minor User investigators as described in this application. These projects will benefit from the requested instrumentation thanks to increased resolution and sensitivity; spatial co-registration across imaging modalities; and the repeatable, non-invasive and minimally-ionizing examination that enables longitudinal imaging to track disease pathogenesis and treatment responses in rodent models. When installed, this instrument will be the only multimodal 3D imaging instrument for in vivo small animal imaging in Milwaukee and southeastern Wisconsin. The BISR is already equipped with the infrastructure and manned by the support staff required for installation and expert operation of the IVIS Spectrum-CT. The new instrument will be maintained by personnel experienced in operating and maintaining in vivo rodent imaging instrumentation, small animal anesthesia, and managing a multi-user recharge facility. Instrument time will be allocated using the existing BISR scheduling system implemented in iLabs. A standing local advisory committee will provide oversight. BISR financial operations are managed by the senior business administrator in the MCW Cancer Center. Acquisition of the IVIS Spectrum-CT instrument requested in this application will have a profound impact on NIH-funded biomedical research programs at MCW and its partner CTSI institutions.