Spinning-Disk Confocal Microscope for Wide-Field, Super-Resolution, and Live-Cell Imaging

用于宽视场、超分辨率和活细胞成像的转盘共焦显微镜

• 批准号: 10415611
• 负责人: Peter G Smith
• 金额: $ 60万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-15 至 2023-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10415611
• 关键词: 3-Dimensional; Area; Autophagocytosis; Axon; Cilia; Computer software; Confocal Microscopy; Coupled; Cytoskeleton; Elements; Funding; Growth; Histologic; Image; Image Analysis; Kansas; Lasers; Medical center; Microscope; Minor; Mitochondria; Optics; Phototoxicity; Preparation; Request for Applications; Research; Research Personnel; Research Project Grants; Resolution; Senior Scientist; Signal Transduction; System; Technical Expertise; Time; United States National Institutes of Health; Universities; design; digital; exosome; imaging facilities; interest; lightspeed; live cell imaging; programs; solid state; trafficking

Project summary This application requests funding for the purchase a Nikon CSU-W1 SoRa Spinning-Disk Super-Resolution Confocal Microscope to support 28 NIH-Funded research projects by 16 major and 7 minor users at the University of Kansas Medical Center (KUMC). The requested system has four solid state lasers and a wide field of view and can image frame rates up to 200fps. The imaging speed and light efficiency of the CSU- W1 SoRa equipped with an environmental chamber and piezoelectric stage make it ideal for live cell imaging, an area of growing interest at KUMC. The increased optical resolution afforded by the microlensing of the SoRa unit together with the additional 3D deconvolution package will enhance spatial resolution capabilities to 120nm with minimal phototoxicity, and will benefit a number of investigators interested in capturing intracellular dynamics in a variety of subcellular systems (e.g., mitochondria, cytoskeleton, exosomes, vesicular trafficking, autophagy, cell-signaling, axonal remodeling, and primary cilia). No other spinning-disk confocal microscopes are present on the KUMC campus, and recent decommissioning of several obsolete confocal systems has led to a critical need. This system will be housed in space designed for and dedicated to confocal microscopy. The CSU-W1 SoRa will be integrated into our long-established Imaging Facility, fully staffed by 2 senior scientists with extensive technical expertise in confocal microscopy and image analysis. Adjacent rooms provide dedicated space for administration, histological preparation, and digital processing on high content image analysis workstations with NIS-Elements software. The Imaging Facility has been successfully facilitating research at KUMC for over 40 years and currently serves about 140 active users. The CSU-W1 SoRa will provide tangible benefit to a large number of programs at a time when recent growth of NIH-funded research at KUMC, coupled with recent reductions confocal capacity, have led to a critical need.

项目摘要 此应用程序请求资助购买尼康CSU-W1索拉旋转磁盘超分辨率 共聚焦显微镜支持28个NIH资助的研究项目，由16个主要用户和7个次要用户在 堪萨斯大学医学中心。所要求的系统具有四个固态激光器和一个宽 视野和可以图像帧速率高达200 fps。CSU的成像速度和光效率- W1索拉配备环境室和压电载物台，是活细胞的理想选择 成像，一个在KUMC越来越感兴趣的领域。由微透镜提供的增加的光学分辨率 索拉单元与额外的3D反卷积包一起将提高空间分辨率 具有最小光毒性的120 nm的能力，并将使许多感兴趣的研究人员受益， 捕获各种亚细胞系统中的细胞内动力学（例如，线粒体，细胞骨架， 外泌体、囊泡运输、自噬、细胞信号传导、轴突重塑和初级纤毛）。没有其他 旋转圆盘共聚焦显微镜目前在KUMC校园，最近退役的 几种过时的共焦系统导致了迫切的需要。该系统将安装在设计为 用于共焦显微镜。CSU-W1索拉将整合到我们长期以来建立的 成像设施，配备有2名在共聚焦显微镜方面具有广泛技术专长的资深科学家 和图像分析。相邻的房间提供给药、组织学制备、 和数字处理的高内容图像分析工作站与NIS-Elements软件。的 成像设备已成功地促进了KUMC的研究超过40年，目前服务于 约140名活跃用户。CSU-W1索拉将为大量项目提供切实的好处， 当最近NIH资助的研究在KUMC的增长，加上最近的共聚焦减少， 能力，导致了一个关键的需求。