Spinning-Disk Confocal Microscope for Wide-Field, Super-Resolution, and Live-Cell Imaging

项目总结 此应用程序请求为购买尼康CSU-W1 SORA旋转磁盘超级分辨率提供资金 共焦显微镜支持NIH资助的28个研究项目，由16个主要用户和7个次要用户在 堪萨斯大学医学中心(KUMC)。所要求的系统有四个固态激光器和一个宽 视场和CAN图像帧速率高达200fps。CSU的成像速度和光效- W1 Sora配备了环境室和压电台，是活细胞的理想选择。 成像，这是KUMC越来越感兴趣的一个领域。微透镜提供的提高的光学分辨率 与额外的3D反卷积组合一起，将提高空间分辨率 具有最小光毒性的120 nm的能力，将使许多感兴趣的研究人员受益 捕捉各种亚细胞系统(如线粒体，细胞骨架， 外体、囊泡运输、自噬、细胞信号、轴突重构和初级纤毛)。没有其他人了 旋转圆盘共聚焦显微镜出现在KUMC园区，最近退役的 几个过时的共焦系统导致了一种迫切的需求。这个系统将被安置在设计好的空间里 为了并致力于共焦显微镜。CSU-W1 SORA将整合到我们历史悠久的 成像设备，配备2名在共焦显微镜方面具有广泛技术专长的资深科学家 和图像分析。相邻房间提供专门的空间用于管理、组织学准备、 使用NIS-Elements软件在高内容图像分析工作站上进行数字处理。这个 40多年来，成像设备成功地促进了KUMC的研究，目前为 大约140名活跃用户。CSU-W1 SORA将在一个月内为大量项目提供切实的好处 最近国立卫生研究院资助的研究在KUMC的增长，加上最近的减少 能力，导致了一种迫切的需求。

Project summary This application requests funding for the purchase a Nikon CSU-W1 SoRa Spinning-Disk Super-Resolution Confocal Microscope to support 28 NIH-Funded research projects by 16 major and 7 minor users at the University of Kansas Medical Center (KUMC). The requested system has four solid state lasers and a wide field of view and can image frame rates up to 200fps. The imaging speed and light efficiency of the CSU- W1 SoRa equipped with an environmental chamber and piezoelectric stage make it ideal for live cell imaging, an area of growing interest at KUMC. The increased optical resolution afforded by the microlensing of the SoRa unit together with the additional 3D deconvolution package will enhance spatial resolution capabilities to 120nm with minimal phototoxicity, and will benefit a number of investigators interested in capturing intracellular dynamics in a variety of subcellular systems (e.g., mitochondria, cytoskeleton, exosomes, vesicular trafficking, autophagy, cell-signaling, axonal remodeling, and primary cilia). No other spinning-disk confocal microscopes are present on the KUMC campus, and recent decommissioning of several obsolete confocal systems has led to a critical need. This system will be housed in space designed for and dedicated to confocal microscopy. The CSU-W1 SoRa will be integrated into our long-established Imaging Facility, fully staffed by 2 senior scientists with extensive technical expertise in confocal microscopy and image analysis. Adjacent rooms provide dedicated space for administration, histological preparation, and digital processing on high content image analysis workstations with NIS-Elements software. The Imaging Facility has been successfully facilitating research at KUMC for over 40 years and currently serves about 140 active users. The CSU-W1 SoRa will provide tangible benefit to a large number of programs at a time when recent growth of NIH-funded research at KUMC, coupled with recent reductions confocal capacity, have led to a critical need.