Ultramicrotome with necessary accessories

超薄切片机及必要配件

• 批准号: 10416530
• 负责人: MARTIN L KATZ
• 金额: $ 15.16万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10416530
• 关键词: 3-Dimensional; Address; Age; Amyotrophic Lateral Sclerosis; Array tomography; Awareness; Biomedical Research; Cellular Structures; Confocal Microscopy; Connective Tissue Diseases; Core Facility; Cytology; Diamond; Disease; Dry Eye Syndromes; Electron Microscopy; Electrons; Equipment; Funding; Glass; Glean; Hereditary Disease; Image; Immunofluorescence Immunologic; Impairment; Individual; Inherited; Investigation; Laboratories; Light; Lysosomal Storage Diseases; Malignant Neoplasms; Microscope; Microscopic; Microscopy; Microtomy; Molecular; Nerve Degeneration; Pathogenesis; Research; Research Personnel; Retina; Retinal Degeneration; Services; Structure; Synapses; Techniques; Three-Dimensional Image; Tissue imaging; Tissues; Training; Universities; Work; digital imaging; effective therapy; image processing; instrument; instrumentation; interest; light microscopy; member; retinal neuron; vibration

Project Summary/Abstract Funds are requested to purchase a Leica ARTOS ultramicrotome with necessary accessories including a vibration isolation instrument table, diamond knives, and a glass knifemaker. Included with the purchase will be training for the major users and the core facility staff member responsible for overseeing use of the instrument. The instrument will be housed in the campus Molecular Cytology Core Facility (MCC) where it will replace an ultramicrotome that is over 30 years old and in disrepair. The existing instrument can no longer be serviced due to its age and lack of replacement parts. The ultramicrotome is essential for the research being conducted by members of a large user group. Research by the users that requires this instrument includes studies on hereditary neurodegenerative and retinal degenerative diseases, amyotrophic lateral sclerosis, dry eye diseases, connective tissue disorders, lysosomal storage diseases and cancer. The new instrument will enable the investigators to prepare tissue sections for both light and electron microscopic (EM) examination. The instrument will enable users to produce ribbons of serial sections that can be used to generate 3- dimensional images of tissues and cellular structures of interest using advanced light microscopy equipment (including confocal microscopes) at the MCC and electron microscopy capabilities of the University Electron Microscopy Core Facility (EMC) to obtain images for array tomography. The 3-D images will enable the investigators to address important research questions that cannot be addressed using microtomy instrumentation currently available at the University. For example, it appears that impaired retinal function in several hereditary disorders under investigation may be due to alterations in the synapses between retinal neurons. It is very difficult to determine whether this is the case by examining individual thin sections with TEM or with immunofluorescence (IF) using confocal microscopy alone. If the users can collect serial sections encompassing entire synaptic structures and generate 3-D composite images that can be examined with both EM and immunofluorescence, the users will be able to reliably determine whether there are disease-related structural alterations in the synapses that could explain the functional deficits. Numerous projects of anticipated users would benefit from having the capability of performing correlative IF and EM with array tomography. The information that users will be able to glean using the ultramicrotome will assist in developing better understanding of pathogenesis and in developing effective therapies for numerous disorders. In addition to benefiting the biomedical research of users that are identified in this proposal, the ultramicrotome has the potential to facilitate the research of additional potential biomedical researchers. Many laboratories utilize the MCC for a variety of microscopy techniques and digital image processing. Through their work in the MCC facility, they will become aware of the availability of the ultramicrotome and its capabilities and may elect to use it in their research.

项目概要/摘要 需要资金购买 Leica ARTOS 超薄切片机及其必要的配件，包括 隔振仪器台、金刚石刀和玻璃刀匠。包含在购买意愿中 对主要用户和负责监督使用的核心设施工作人员进行培训 乐器。该仪器将安装在校园分子细胞学核心设施 (MCC) 中 更换使用超过 30 年且年久失修的超薄切片机。现有仪器不能再 由于其老化和缺乏更换零件而进行维修。超薄切片机对于研究至关重要 由大型用户组的成员进行。需要该仪器的用户的研究包括 遗传性神经退行性疾病和视网膜退行性疾病、肌萎缩侧索硬化症、干性 眼部疾病、结缔组织疾病、溶酶体贮积病和癌症。新仪器将 使研究人员能够准备用于光学和电子显微镜 (EM) 检查的组织切片。 该仪器将使用户能够生产可用于生成 3- 使用先进的光学显微镜设备获得感兴趣的组织和细胞结构的三维图像 电子大学MCC和电子显微镜能力（包括共焦显微镜） 显微镜核心设施 (EMC)，用于获取阵列断层扫描图像。 3D 图像将使 研究人员解决使用切片术无法解决的重要研究问题 大学现有仪器。例如，视网膜功能受损 正在研究的几种遗传性疾病可能是由于视网膜之间突触的改变引起的 神经元。通过使用 TEM 检查单个薄片很难确定情况是否如此 或单独使用共聚焦显微镜进行免疫荧光 (IF)。如果用户可以收集连续部分 涵盖整个突触结构并生成 3D 合成图像，可以使用两者进行检查 EM和免疫荧光，用户将能够可靠地确定是否存在与疾病相关的 突触的结构改变可以解释功能缺陷。众多项目备受期待 用户将受益于通过阵列断层扫描执行相关 IF 和 EM 的能力。这 用户将能够使用超薄切片机收集的信息将有助于更好地开发 了解发病机制并开发针对多种疾病的有效疗法。此外 超薄切片机有利于本提案中确定的用户的生物医学研究， 促进其他潜在生物医学研究人员的研究的潜力。许多实验室利用 MCC 适用于各种显微技术和数字图像处理。通过他们在 MCC 的工作 设施，他们将意识到超薄切片机的可用性及其功能，并可能选择使用 在他们的研究中。