Bioluminescence and Fluorescence Optical Animal Imager

项目摘要/摘要 Perkin Elmer IVIS Spectrum所需的是一台能够进行2D操作的全动物光学成像仪 以及三维荧光和生物发光成像。该仪器提供了高性能 吞吐量(一次最多10只小鼠)、高灵敏度(低至单细胞检测)和 使动物在成像过程中保持麻醉和温暖的生理平台。喜欢 其他IVIS仪器，IVIS光谱以绝对辐射单位校准，允许 样品在不同仪器中成像的比较。这台仪器将会 更换供应商不再支持的已有18年历史的光学动物成像仪。 超过32个实验室依赖于目前的仪器，他们的研究将是 如果仪器发生故障，将受到灾难性的影响。在这些实验室中，有10个NIH- 资助的调查人员是这项提案的使用者。能够提供这些 使用Perkin Elmer IVIS Spectrum动物成像仪的调查人员将保护他们的 目前的生物发光能力和恢复的荧光成像能力 在过去是可用的。此外，新仪器具有更好的灵敏度和多模式 灵活性。这样加强的研究项目将涵盖免疫系统的研究。 发展和自身免疫，艾滋病毒中枢神经系统储存库的监测，免疫治疗 癌症，乳腺癌和前列腺癌的抗肿瘤药物输送，以及治疗 缺血性中风和杜申氏肌营养不良症。

Project Summary/Abstract The Perkin Elmer IVIS Spectrum requested is a whole animal optical imager capable of 2D and 3D fluorescence and bioluminescence imaging. The instrument provides high throughput (up to 10 mice at a time), high sensitivity (down to single cell detection), and a physiological platform that keeps the animals anesthetized and warm during imaging. Like other IVIS instruments, the IVIS Spectrum is calibrated in absolute radiance units, allowing comparison between samples imaged in different instruments. This instrument will replace an 18-year-old optical animal imager that is no longer supported by the vendor. More than 32 laboratories depend on the current instrument, and their research would be catastrophically affected if the instrument failed. Among those laboratories are the 10 NIH- funded investigators that are the users in this proposal. Being able to provide these investigators with the Perkin Elmer IVIS Spectrum animal imager would protect their current capabilities of bioluminescence and restore the fluorescence imaging capabilities available in the past. In addition the new instrument has better sensitivity and multimodal flexibility. The research projects thus enhanced will cover studies of immune system development and autoimmunity, surveillance of HIV CNS reservoirs, immune therapy of cancer, anti-tumor drug delivery in mammary and prostate cancer, and therapies for ischemic stroke and Duschenne’s muscular dystrophy.