Bioluminescence and Fluorescence Optical Animal Imager

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Project Summary/Abstract The Perkin Elmer IVIS Spectrum requested is a whole animal optical imager capable of 2D and 3D fluorescence and bioluminescence imaging. The instrument provides high throughput (up to 10 mice at a time), high sensitivity (down to single cell detection), and a physiological platform that keeps the animals anesthetized and warm during imaging. Like other IVIS instruments, the IVIS Spectrum is calibrated in absolute radiance units, allowing comparison between samples imaged in different instruments. This instrument will replace an 18-year-old optical animal imager that is no longer supported by the vendor. More than 32 laboratories depend on the current instrument, and their research would be catastrophically affected if the instrument failed. Among those laboratories are the 10 NIH- funded investigators that are the users in this proposal. Being able to provide these investigators with the Perkin Elmer IVIS Spectrum animal imager would protect their current capabilities of bioluminescence and restore the fluorescence imaging capabilities available in the past. In addition the new instrument has better sensitivity and multimodal flexibility. The research projects thus enhanced will cover studies of immune system development and autoimmunity, surveillance of HIV CNS reservoirs, immune therapy of cancer, anti-tumor drug delivery in mammary and prostate cancer, and therapies for ischemic stroke and Duschenne’s muscular dystrophy.