Mitochondrial mechanisms of maternal age effects on offspring health and lifespan

母亲年龄影响后代健康和寿命的线粒体机制

• 批准号: 10418989
• 负责人: Kristin Gribble
• 金额: $ 34.75万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-15 至 2027-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10418989
• 关键词: Age; Aging; Animals; Area; Autophagocytosis; Behavior; Biochemical; Biogenesis; Biological Models; Caring; Cells; Communication; Complex; Development; Disease; Embryo; Embryonic Development; Etiology; Female; Fertility; Functional disorder; Gene Expression; Genes; Health; Homeostasis; Human; Image; Incidence; Inherited; Invertebrates; Investments; Literature; Longevity; Maternal Age; Measures; Metabolic; Metabolism; Microscopic; Mitochondria; Mitochondrial DNA; Modeling; Molecular; Mothers; Nerve Degeneration; Nuclear; Outcome; Oxidation-Reduction; Oxidative Phosphorylation; Pharmacologic Substance; Pharmacology; Phenotype; Play; Population; Production; Public Health; RNA Interference; Reactive Oxygen Species; Reproduction; Research; Resistance; Role; Signal Transduction; Source; Stress; Structure; System; Testing; Time; Work; advanced maternal age; age effect; age related; aged; cell injury; cell motility; comparative; early onset; experimental study; female fertility; fitness; healthspan; imaging approach; improved; innovation; inter-individual variation; intergenerational; male; mitochondrial dysfunction; mitochondrial metabolism; negative affect; offspring; postnatal; reproductive; response; targeted treatment; transcriptome sequencing; young mother

7. PROJECT SUMMARY Advanced maternal age at the time of reproduction decreases offspring lifespan and health in a range of species, including humans. The mechanisms controlling maternal age effects on offspring are unknown, however. Mitochondria are prone to damage and dysfunction with age and are maternally inherited, suggesting they may play a role in intergenerational maternal effects on offspring health. Our work shows that offspring from older mothers have shorter lifespan, lower reproduction, decreased health, and altered behavior relative to young- mother offspring. We found that these changes are associated with higher mtDNA per cell, more mitochondria, larger mitochondria, decreased mitochondrial intermembrane area, lower oxidative potential, lower ATP content, and altered reactive oxygen species (ROS) levels. Our results and the literature on mitochondria in aging led to our hypothesis that mitochondrial dysfunction in advanced maternal age causes accumulation of dysfunctional mitochondria in offspring through compensatory biogenesis and decreased autophagy during development. This disrupts offspring mitochondrial function and mitochondrial-nuclear communication, leading to accelerated offspring aging. The objective of this project is to understand the mitochondrial mechanisms by which maternal age determines offspring aging. In this study, we will: (1) identify the maternal mitochondrial mechanisms that trigger maternal age effects; (2) identify the developmental mechanisms causing accumulation of mtDNA and damaged mitochondria in old-mother offspring; and (3) identify the offspring mitochondrial mechanisms involved in negative maternal age effects and determine if these mechanisms are ROS-dependent. This work will be accomplished using qPCR and RNA-Seq to quantify mtDNA and controls on metabolism, signaling, and dynamics; imaging to quantify mitochondrial ultrastructure and mitophagy, and pharmacological and RNAi manipulation of maternal and offspring mitochondria to test mechanisms. We will use biochemical, respirometry, and imaging approaches to measure mitochondrial efficiency. Additional biochemical and imaging approaches will be used to quantify ROS and cellular damage. We will use lifetable experiments to measure lifespan and reproduction in response to maternal age or mechanistic tests. Studies will be on both female and male offspring. Our study system is the rotifer Brachionus manjavacas—a short-lived, aquatic, microscopic invertebrate with unique advantages as a model for investigating maternal age effects. Our approach will identify the cellular mechanisms by which advanced maternal age causes accumulation of mtDNA and damaged mitochondria in offspring; changes offspring mitochondrial dynamics, structure, and function; and decreases offspring fitness. If successful, this research will advance the field by revealing drivers of accelerated aging in offspring due to maternal age effects and by identifying mechanisms underlying a poorly understood cause of interindividual variability in healthspan and lifespan. Our findings will have implications for understanding age-related changes in female fertility and for identifying potential targets for treatment of age-related dysfunction.

7. 项目总结