Salivary gland response to Desert hedgehog signaling as an antidote to damage from therapeutic radiation
唾液腺对沙漠刺猬信号的反应作为治疗辐射损伤的解毒剂
基本信息
- 批准号:10420976
- 负责人:
- 金额:$ 52.57万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-03-15 至 2027-01-31
- 项目状态:未结题
- 来源:
- 关键词:ATAC-seqAblationAcinar CellAdultAdverse effectsAgingAntidotesArtificial SalivaAtrophicBladderCell LineageCell physiologyCell surfaceCellsClinicalDataDeglutitionDigestionDiseaseEngineeringEpigenetic ProcessEpithelialEpithelial CellsErinaceidaeEsthesiaExposure toFamily memberFeedbackFunctional disorderGeneticGenetic ModelsGoalsHead and Neck CancerHomeostasisHost DefenseHumanIn Situ HybridizationInjuryIntestinesKnowledgeLigandsMaintenanceMajor salivary gland structureMediatingMesenchymalMesenchymeMethodsMinorMolecularMolecular ConformationMusMyoepithelial cellNatural regenerationOralOral cavityOral healthOral mucous membrane structureOrganParotid GlandPathway interactionsPharmaceutical PreparationsPharmacologyPhysiologicalPlayProcessRadiationRadiation InjuriesRadiation therapyRecoveryRegenerative capacityResearchResidual stateResolutionRiskRoleSalivaSalivary Gland TissueSalivary GlandsSignal TransductionSignaling ProteinSjogren&aposs SyndromeSpeechSubmandibular glandSumSupporting CellTaste PerceptionTestingTherapeuticTissuesWell in selfWorkXerostomiacancer therapycell typegenome-widein vivoinjury and repairirradiationmacrophagemouse geneticsmouse modelnanobodiesnerve supplynovel strategiespreservationprogenitorprogramsradiation recoveryreceptorregenerativerepairedresponserestorationsaliva secretionsalivary cellside effectsmoothened signaling pathwaytherapeutic targettissue repairtranscriptomicstumorunpublished works
项目摘要
PROJECT SUMMARY/ABSTRACT
Salivation is a critical physiological activity that aids digestion, maintains oral health, and supports functions such
as speech, swallowing and taste sensation. Salivary gland dysfunction results from ageing, diseases such as
Sjögren syndrome and from radiotherapy for head and neck cancers. Therapeutic irradiation causes permanent
damage to salivary glands, highlighting their poor regenerative ability. One potential obstacle to recovery of
salivation may be that damage, particularly during radiation therapy, is inflicted not only on saliva-generating
epithelial cells but also on supporting mesenchymal cells. Indeed, preservation or restoration of mesenchymal
cell function may constitute an ideal therapeutic target, as an optimized mesenchymal microenvironment may
augment the function and regenerative capacity of residual salivary gland epithelial cells and their progenitors.
A knowledge of how mesenchymal cells function during homeostasis and contribute to regeneration after injury
thus may provide a new approach to activate mechanisms that protect salivary glands and enhance their repair.
In many organs the mesenchymal expression of signals that provide regenerative feedback to the epithelium
during homeostasis and injury repair is induced by expression of a Hedgehog (Hh) protein signal from the
epithelium. Using mouse genetic models, cell lineage tracing, and single-cell transcriptomics, we have
discovered that Desert hedgehog (DHH), the least studied of the three mammalian Hh family members, drives
an epithelial-mesenchymal feedback (EMF) circuit in the major adult salivary glands, and that activity of this
circuit is crucial for salivary gland maintenance and for regeneration after radiation injury. Importantly, although
DHH expression in cells of the salivary gland epithelium is essential for regeneration, our findings also highlight
a vital role for mesenchymal response to this signal for execution of the regenerative program.
Here we propose to elucidate the role of Hh signaling in salivary gland homeostasis and regeneration by
characterizing at a single cell level the transcriptomic and epigenetic consequences of EMF circuit activity, and
to assess the conservation of DHH-driven EMF circuitry in human salivary glands. With the goal of manipulating
Hh pathway activity for protection from or enhancement of tissue repair after radiation injury, we have developed
a conformation-specific nanobody against the Hh receptor Patched1 that activates Hh pathway response. This
nanobody can be targeted to specific cell and tissue types, thus mitigating potential adverse effects arising from
systemic Hh pathway activation. With this agent, we will test the possibility that precise, tissue-targeted activation
of the Hh pathway can effectively enhance endogenous reparative mechanisms for salivary gland protection
from and restoration after injury from irradiation like that administered in head and neck cancer therapy.
项目总结/文摘
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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PHILIP A BEACHY其他文献
PHILIP A BEACHY的其他文献
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{{ truncateString('PHILIP A BEACHY', 18)}}的其他基金
Signal integration by specialized mesenchyme in urothelial homeostasis and Interstitial Cystitis / Bladder Pain Syndrome
尿路上皮稳态和间质性膀胱炎/膀胱疼痛综合征中特殊间充质的信号整合
- 批准号:
10583133 - 财政年份:2022
- 资助金额:
$ 52.57万 - 项目类别:
Salivary gland response to Desert hedgehog signaling as an antidote to damage from therapeutic radiation
唾液腺对沙漠刺猬信号的反应作为治疗辐射损伤的解毒剂
- 批准号:
10592398 - 财政年份:2022
- 资助金额:
$ 52.57万 - 项目类别:
Hedgehog signaling in taste cell maintenance and regeneration
味觉细胞维持和再生中的刺猬信号传导
- 批准号:
10394796 - 财政年份:2018
- 资助金额:
$ 52.57万 - 项目类别:
Hedgehog signaling in taste cell maintenance and regeneration
味觉细胞维持和再生中的刺猬信号传导
- 批准号:
9918153 - 财政年份:2018
- 资助金额:
$ 52.57万 - 项目类别:
Hedgehog signaling in taste cell maintenance and regeneration
味觉细胞维持和再生中的刺猬信号传导
- 批准号:
9066827 - 财政年份:2015
- 资助金额:
$ 52.57万 - 项目类别:
Hedgehog signaling in taste cell maintenance and regeneration
味觉细胞维持和再生中的刺猬信号传导
- 批准号:
8954956 - 财政年份:2015
- 资助金额:
$ 52.57万 - 项目类别:
Molecular mechanisms of Hedgehog receptor function
Hedgehog受体功能的分子机制
- 批准号:
8640198 - 财政年份:2012
- 资助金额:
$ 52.57万 - 项目类别:
Molecular mechanisms of Hedgehog receptor function
Hedgehog受体功能的分子机制
- 批准号:
8849924 - 财政年份:2012
- 资助金额:
$ 52.57万 - 项目类别:
Molecular mechanisms of Hedgehog receptor function
Hedgehog受体功能的分子机制
- 批准号:
10737476 - 财政年份:2012
- 资助金额:
$ 52.57万 - 项目类别:
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