Super-resolution spinning disk confocal microscope for Dartmouth Life Sciences
用于达特茅斯生命科学的超分辨率转盘共焦显微镜
基本信息
- 批准号:10427997
- 负责人:
- 金额:$ 60万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-05-01 至 2023-04-30
- 项目状态:已结题
- 来源:
- 关键词:AnimalsBiological SciencesCell Culture TechniquesCellsColorCommunitiesConfocal MicroscopyDiseaseEnsureEquipmentFundingFutureGoalsGrantImageKidney DiseasesLaboratoriesLasersMalignant NeoplasmsMammalian CellMicroscopeMicroscopyMinorMycosesNoiseOpticsPhotonsPhototoxicityPhysiologyRecording of previous eventsResearchResearch PersonnelResolutionSamplingServicesSignal TransductionSpeedSystemTechnologyTissue SampleTrainingUnited States National Institutes of HealthYeastsautism spectrum disordercollegedevelopmental diseaseexperiencefluorescence imagingflyimaging capabilitiesinnovationinstrumentlight microscopymedical schoolstemporal measurement
项目摘要
PROJECT ABSTRACT
Twelve NIH-funded investigators from five departments at Dartmouth College and the Geisel School of Medicine
request funds to purchase a Yokogawa CSU-W1 SoRa spinning disk confocal microscope from Nikon (Nikon
SoRa) that would enable rapid, super-resolution, multi-channel imaging of fluorescent live cell and tissue
samples. The requested system integrates simultaneous two-color imaging at up to 200 frames per second, X-
Y resolution to 120 nm, dynamic photostimulation enabled by an XY galvo high-speed point scanner and four
lasers (405 nm, 488 nm, 561 nm, and 640 nm). This system is suitable for a wide range of live and fixed sample
applications including whole animal metazoan systems (fly, worm), mammalian cell culture, and yeast. The eight
Major Users and four Minor Users are supported by 18 NIH grants to conduct innovative research aimed at
elucidating the mechanisms that underlie normal physiology and disease states, including cancer, autism,
developmental disorders, degenerative kidney disease and fungal infection. Existing systems at Dartmouth do
not fulfill the needs for both high spatial and temporal resolution necessary for the future expansion of these
projects. The Nikon SoRa is an optimal solution to fulfill these unmet imaging needs of the Major/Minor Users
and was selected to achieve two closely related technical goals: 1) to provide a solution for high-speed, super-
resolution imaging of live and fixed samples that is currently unattainable with existing equipment; and 2) to
reduce phototoxicity and photodamage without sacrificing resolution and signal-to-noise ratio for the long-term
imaging of sensitive or weakly expressing live samples. By adding optical photon reassignment to the spinning
disk technology and using deconvolution to optimize resolution, the Nikon SoRa system provides a unique
combination of super-resolution imaging with high-speed acquisition. The requested confocal microscopy system
would therefore be a one-of-a-kind instrument at Dartmouth and revolutionize imaging capability for the
Dartmouth life sciences community. The microscope will be housed in and maintained by the centrally-located
Life Sciences Light Microscopy Facility, which has a long history of supporting life sciences research and is
utilized by over 50 laboratories that are largely NIH-supported, thereby ensuring widespread access for all
Dartmouth investigators and strong impact on NIH-funded research. Dartmouth College and the Geisel School
of Medicine have committed $100,000 in institutional support for this purchase, as well as the services of three
imaging experts with extensive experience in providing imaging support and training, which will ensure that the
requested SoRa microscopy system remains supported and available for Dartmouth researchers for years to
come.
项目摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Yasmath Ahmed其他文献
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{{ truncateString('Yasmath Ahmed', 18)}}的其他基金
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10217057 - 财政年份:2020
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10653134 - 财政年份:2020
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10417184 - 财政年份:2020
- 资助金额:
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- 资助金额:
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