Creating a niche for engineered live biotherapeutics

为工程活生物治疗创造利基市场

基本信息

  • 批准号:
    10427447
  • 负责人:
  • 金额:
    $ 29.07万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2021
  • 资助国家:
    美国
  • 起止时间:
    2021-07-01 至 2026-05-31
  • 项目状态:
    未结题

项目摘要

PROJECT SUMMARY The long-term goal of this proposal is to improve outcomes of delivery of small molecules, peptides, and proteins to the human gut by engineered live biotherapeutic bacteria. To accomplish this, we will use two strategies to optimize biotherapeutic growth in the gut environment. First, we will match prebiotic compounds (complex carbohydrates not digested by the body) with heterologous expression of enzymes that allow cells to utilize these privileged carbon sources. Second, we will create genome-scale libraries in our candidate biotherapeutics, E. coli Nissle 1917 and Lactococcus lactis, to identify gene products that increase fitness in the gut and gut-like environments. The methods will be tested in in vitro culture, small intestinal organoid co-culture, and in vivo in the mouse gut to determine enrichment of the biotherapeutic population, enhanced production of the therapeutic small molecule, peptide, or protein of interest, and phenotypic outcome in systems of increasing verisimilitude to the human gut.
项目总结

项目成果

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Thomas J Mansell其他文献

Thomas J Mansell的其他文献

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{{ truncateString('Thomas J Mansell', 18)}}的其他基金

Location-specific In Vivo Sensing and Imaging of Butyrate in the GI Tract
胃肠道中丁酸盐的特定位置体内传感和成像
  • 批准号:
    10433447
  • 财政年份:
    2022
  • 资助金额:
    $ 29.07万
  • 项目类别:
Location-specific In Vivo Sensing and Imaging of Butyrate in the GI Tract
胃肠道中丁酸盐的特定位置体内传感和成像
  • 批准号:
    10611459
  • 财政年份:
    2022
  • 资助金额:
    $ 29.07万
  • 项目类别:
Creating a niche for engineered live biotherapeutics
为工程活生物治疗创造利基市场
  • 批准号:
    10276995
  • 财政年份:
    2021
  • 资助金额:
    $ 29.07万
  • 项目类别:
Creating a niche for engineered live biotherapeutics
为工程活生物治疗创造利基市场
  • 批准号:
    10622534
  • 财政年份:
    2021
  • 资助金额:
    $ 29.07万
  • 项目类别:
Co-culture of probiotic bacteria for growth factor delivery in minigut organoids
共培养益生菌以在小肠类器官中递送生长因子
  • 批准号:
    9789051
  • 财政年份:
    2018
  • 资助金额:
    $ 29.07万
  • 项目类别:
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