Location-specific In Vivo Sensing and Imaging of Butyrate in the GI Tract

项目摘要 短链脂肪酸丁酸盐是人体肠道代谢组中的关键分子， 调节细菌生长、炎症和结肠细胞健康。尽管它很重要 目前还没有可靠的方法在线监测丁酸盐的生产现场。 此外，盲肠和粪便中丁酸盐的水平通常不能很好地预测丁酸盐。 由肠道细菌产生。这项提议的目标是建造细菌生物传感器 丁酸盐，耦合到蜂窝存储电路，将能够监测丁酸盐 整个小肠和大肠的内容和生物地理图谱 丁酸盐浓度。这项技术将允许研究人员测试许多不同的 干预措施(例如，益生菌、益生菌和其他形式的丁酸盐补充) 目标是优化肠道中丁酸的水平。我们还将测试 增加丁酸盐的干预措施，作为该方法的概念证明。

Project Summary The short chain fatty acid butyrate is a key molecule in the human gut metabolome, regulating bacterial growth, inflammation, and colon cell health. Despite its importance there is currently no reliable method for inline monitoring of butyrate production in situ. In addition, cecal and fecal levels of butyrate are often not good predictors of butyrate production by gut bacteria. The goal of this proposal is to build bacterial biosensors for butyrate which, coupled to cellular memory circuits, will enable monitoring of butyrate content throughout the small and large intestine and biogeographical mapping of butyrate concentrations. This technology will allow researchers to test many different interventions (e.g., prebiotics, probiotics, and other forms of butyrate supplementation) with the goal of optimizing the levels of butyrate in the gut. We will also test interventions that increase butyrate as a proof of concept of the method.