High-resolution ex vivo microCT for biomedical imaging

用于生物医学成像的高分辨率离体 microCT

• 批准号: 10426416
• 负责人: Ali Murat Maga
• 金额: $ 41.33万
• 依托单位: SEATTLE CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2023-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10426416
• 关键词: 14 year old; 3-Dimensional; Affect; Area; Back; Basic Science; Biology; Child; Communities; Computer software; Contract Services; DNA Sequence Alteration; Data; Data Storage and Retrieval; Development; Devices; Dimensions; Equipment; Fred Hutchinson Cancer Research Center; Funding; Goals; Grant; Human; Human Resources; Image; Image Analysis; Injury; Institutes; Institution; Maintenance; Manuals; Microscopic; Minor; Organ; Pathology; Phenotype; Preparation; Productivity; Research; Research Institute; Research Personnel; Research Project Grants; Resolution; Resources; Sampling; Specimen; Syndrome; System; Systems Biology; Three-Dimensional Image; Time; Tissue imaging; United States National Institutes of Health; Universities; Washington; Whole Organism; Work; base; biomedical imaging; bone; data acquisition; end of life; imaging system; in vivo; innovation; instrument; microCT; microscopic imaging; new technology; tissue regeneration

Project Summary The goal of this application is to replace an essential, 14 year old in-vivo microCT imaging system at the Seattle Children’s Research Institute (SCRI) with a state-of-the-art Bruker Skyscan 1272 high-resolution 3D ex- vivo microCT imaging system. This instrument is required to conduct a diversity of research projects, which require imaging both at large-scale (whole organisms/organs) and microscopic-scale. This imaging resource will support the activities of 11 major and 4 minor users focused on three cross-cutting research themes: (1) Genetic mutations underlying human congenital syndromes that affect multiple organs, (2) development and pathology of vasculature, (3) mechanisms of tissue regeneration after ischemic damage or injury, and (4) basic research in bone biology and remodeling. A portfolio of 18 funded and 4 pending NIH grants, as well as funding from other federal agencies like NSF currently supports these projects. This exciting new technology allow users to rake benefits from the technological improvements introduced to microCT imaging and sample preparation in the last decade. As shown in our pilot data, with the new ex-vivo system we will be able to image tissues with higher resolution, with better image quality and clarity compared to our current end-of-life instrument. Further, some of the listed projects involve tedious phenotypic screens, which have been hampered by the manual sample navigation and data acquisition with our existing system. With an automated sample changer and a control software that automatically determines the optimal settings, based specimen dimensions, the proposed equipment will help our users overcome these problems, and achieve higher analytical throughput, while obtaining better quality data. There is strong Institutional commitment for the instrument, including state-of-the-art imaging space, coverage of service contracts, maintenance personnel effort in at least the first 5 years, backed-up data storage, and 3-D image analysis resources. While SCRI and University of Washington investigators comprise the main user group, these instruments would be accessible to other investigators from allied institutions such as the Fred Hutchinson Cancer Research Center, Institutes for Systems Biology and others, creating a unique and valuable community resource for macroscopic-to- microscopic imaging. In addition, there is extensive imaging expertise present at the SCRI Center that will house and support the device (PI, Maga, Co-I, Shih and Yu). This will guide the proper use, time allocation and maintenance of the instrument. This imaging resource would immediately influence the productivity and innovation of ongoing work and will be critical in furthering the long-range objectives of the biomedical community in the greater Seattle area.

项目摘要 该应用的目标是替换一个基本的，14岁的体内microCT成像系统， 西雅图儿童研究所（SCRI）拥有最先进的布鲁克Skyscan 1272高分辨率3D前视系统， 活体显微CT成像系统。这一工具需要进行各种研究项目， 需要在大尺度（整个生物体/器官）和显微尺度下成像。这个图像资源 将支持11个主要用户和4个次要用户的活动，重点是三个交叉研究主题： 影响多个器官的人类先天性综合征的基因突变，（2）发育和 血管病理学，（3）缺血性损伤或损伤后组织再生的机制，和（4）基础 研究骨骼生物学和重塑。18个已资助和4个待批准的NIH赠款组合，以及 来自其他联邦机构如NSF的资金目前支持这些项目。这项令人兴奋的新技术 使用户能够从microCT成像和样品的技术改进中获益 在过去的十年里，准备。如我们的试验数据所示，使用新的体外系统，我们将能够成像 与我们目前的生命末期相比，具有更高分辨率、更好的图像质量和清晰度的组织 仪器此外，所列项目中的一些涉及繁琐的表型筛选，其已经被广泛应用。 受我们现有系统的手动样品导航和数据采集的阻碍。与自动 自动进样器和控制软件，可根据样品自动确定最佳设置 尺寸，建议的设备将帮助我们的用户克服这些问题，并实现更高的 分析吞吐量，同时获得更高质量的数据。有强有力的机构承诺， 仪器，包括最先进的成像空间，服务合同的覆盖范围，维护人员 至少在前5年的努力，备份数据存储和3-D图像分析资源。虽然SCRI和 华盛顿大学的研究人员组成了主要的用户群，这些仪器将是可访问的 其他研究人员从联盟机构，如弗雷德哈钦森癌症研究中心，研究所 为系统生物学和其他人，创造一个独特的和有价值的社区资源，为宏观到 显微成像此外，SCRI中心拥有广泛的成像专业知识， 容纳并支撑器械（PI、Maga、Co-I、Shih和Yu）。这将指导正确的使用，时间分配和 仪器的维护。这种成像资源将立即影响生产率， 创新正在进行的工作，并将在促进生物医学的长期目标至关重要， 大西雅图地区的社区。