Development of non-contact rheometry for measuring rheological properties of biological fluids

开发用于测量生物液体流变特性的非接触式流变仪

• 批准号: 10426636
• 负责人: Hsiao-Chuan Liu
• 金额: $ 0.62万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-01 至 2022-07-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10426636
• 关键词: Acoustics; Address; Air; Behavior; Biological; Biological Markers; Biology; Blood; Blood Viscosity; Blood capillaries; COVID-19; Clinical; Coronary heart disease; Custom; Development; Devices; Diagnosis; Disease; Disease Progression; Environment; Frequencies; Glycerol; Goals; Hematocrit procedure; Hematological Disease; Industrialization; Laboratories; Link; Liquid substance; Lung; Measurement; Measures; Methods; Motion; Mucous body substance; Optical Coherence Tomography; Pathologic Processes; Pattern; Peripheral arterial disease; Phase; Physiologic pulse; Physiological Processes; Plasma; Problem Solving; Property; Publications; Radiation; Reporting; Research; Resolution; Rotation; Sampling; Scanning; Source; Stroke; Surface; Surface Tension; System; Techniques; Testing; Theoretical model; Thinness; Time; Touch sensation; Transducers; Tube; Viscosity; Water; Work; base; data acquisition; experimental study; hyperviscosity syndrome; instrument; particle; respiratory distress syndrome; signal processing; ultrasound

ABSTRACT/SUMMARY Background: Rheological properties of biological fluids are closely linked with various physiological processes. For example, disorders of blood viscosity are significantly associated with the progression of coronary heart disease, peripheral artery diseases, stroke and hyperviscosity syndromes. The surface tension of mucus is influenced by pathological processes in the lungs. While rheological properties of fluids are important biomarkers critical to diagnose and evaluate progression of diseases, rheometry instruments have been primarily developed for industrial applications. Existing rotational-based and tube-based rheometry devices are not capable of measuring both surface tension and viscosity of fluids. Additionally, there are various drawbacks of current rheometry instruments for measuring viscosity and methods for measuring surface tension such as the need for contacting samples, necessitating highly skilled operators, and cleaning the testing chamber between each sample. This project aims to address these unmet and critical needs by developing a non-contact rheometry method for measuring biological fluid surface tension and viscosity using small volume samples (thin- layer fluid). Methods: We will utilize ultrasound as an excitation source or “acoustic indenter” to generate a propagating capillary wave on the surface of the fluid and use optical coherence tomography (OCT) as a measurement device to precisely record particle displacements of wave motion. With this experimental approach, the sample in a Petri dish is never in direct contact with any part of the measurement apparatus. Our previous work has utilized capillary waves in a deep fluid regime to measure surface tension and viscosity. We will build on this previous work to extend the theoretical model into the thin-layer fluid case, capillary waves in a shallow fluid regime, in a similar non-contact fashion. We will optimize the proposed rheometry method to achieve measurements with high accuracy and precision for thin-layer measurements. To accomplish the goals of this project we propose these Specific Aims: • Aim 1) Construct and validate the theoretical model for rheological properties in the shallow fluid case. • Aim 2) Optimize the rheometry acquisition method and signal processing to yield robust results in thin fluid layers. Impact: The proposed technique will carry out measurements with the following advantages including being non-contact, fully automated, fast, and not needing cleaning between tests, which provides a biology-friendly environment.

摘要/摘要 背景：生物体液的流变性与多种生理因素密切相关 流程。例如，血液粘度的紊乱与糖尿病的进展密切相关。 冠心病、外周动脉疾病、中风和高粘滞综合征。表面张力 粘液的含量受肺部病理过程的影响。而流体的流变性是 对诊断和评估疾病进展至关重要的重要生物标记物，流变仪有 主要是为工业应用开发的。现有的基于旋转和基于管的流变仪 仪器不能同时测量液体的表面张力和粘度。此外，还有 当前测量粘度的流变仪的种种缺陷及其测量方法 表面张力，例如需要接触样品、需要高技能的操作员和清洁 每个样品之间的测试室。 该项目旨在通过开发一种非接触式流变仪来满足这些未得到满足的和关键的需求 用小体积样品测量生物液体表面张力和粘度的方法(薄层样品) 层流体)。 方法：我们将利用超声波作为激励源或“声压头”来产生传播的声场 利用光学相干层析成像技术(OCT)测量流体表面的毛细管波 精确记录波动的质点位移的装置。通过这种实验方法，样本 在培养皿中，绝对不会与测量仪器的任何部件直接接触。我们之前的工作是 利用深部流体中的毛细管波测量表面张力和粘度。我们将在此基础上继续努力 以前的工作将理论模型扩展到薄层流体的情况下，浅层流体中的毛细管波 政权，以类似的非接触方式。我们将优化建议的流变学方法，以实现 用于薄层测量的高准确度和精密度的测量。 为了实现该项目的目标，我们提出以下具体目标： ·目的1)建立和验证浅海流变性理论模型 流畅的箱子。 ·目标2)优化流变仪采集方法和信号处理，以产生稳健的结果 在薄薄的流体层中。 影响：拟议的技术将进行测量，具有以下优势： 非接触式，全自动，快速，不需要在测试之间进行清洁，这提供了一种生物友好型 环境。