UCLA IDDRC: Structural and Functional Visualization Core

核心E：结构和功能可视化 Samantha Butler，核心总监; Peyman Golshani，核心联席总监; Neil Harris和Susan Bookheimer; MRI次核心总监 摘要 结构和功能可视化核心提供全面的成像服务， 加州大学洛杉矶分校智力与发育障碍研究中心（IDDRC）的成员，工作 遗传和环境引起的发育疾病的任何方面， 神经系统的发育和功能。加州大学洛杉矶分校IDDRC正在进行的研究跨越了 基础科学家使用还原论的方法来阐明知识分子特有的机制， 发展障碍（IDDs）的临床医生评估患者的治疗干预。到 满足他们的所有成像要求，我们提供三光显微镜 核心，包括一个专用于IDDRC研究人员的显微镜套件，人类和动物MRI 启动和完成任何成像分析所需的设施和技术支持。 结构和功能可视化核心还致力于开发新技术 用于可视化生物样本，并将其提供给IDDRC研究人员。在这一提议中， 我们专注于[1]开发更小更轻的单光子微型荧光显微镜 用于自由移动动物的神经活动的实时成像，[2]改进了用于神经活动的方法。 和iDISCO，使组织透明，从而允许无与伦比的视力协议 进入大脑的复杂回路。这些技术提供了一个整体的方法的承诺 成像，允许IDDRC研究人员将机制转化为治疗。比如说， 研究特定智力障碍的研究人员将能够对患者进行MRI， 识别大脑的受影响区域，在啮齿动物模型中植入微型显微镜， 在体内进行Ca 2+成像，以检查特定神经元群体的放电模式， 被疾病机械地改变，同时检查假定的异常回路 形成使用光学显微镜结合结晶度。 最后，该核心还支持所有其他核心的工作，提供IDDRC 研究人员能够探测分子和细胞功能在任何水平上从亚细胞， 细胞到活的动物，并确定治疗干预的后果。

CORE E: STRUCTURAL AND FUNCTIONAL VISUALIZATION Samantha Butler, Core Director; Peyman Golshani, Core Co-Director; Neil Harris and Susan Bookheimer; MRI sub-core directors Abstract The Structural and Functional Visualization Core provides comprehensive imaging services to the members of UCLA Intellectual & Developmental Disabilities Research Center (IDDRC), working on any aspect of the genetic and environmentally-induced developmental diseases affecting nervous system development and function. The research ongoing in the UCLA IDDRC spans basic scientists using reductionist approaches to elucidate the mechanisms specific to intellectual developmental disabilities (IDDs) to clinicians assessing therapeutic interventions for patients. To accommodate all of their imaging requirements, we provide access to three light microscopy cores, including a microscopy suite dedicated to IDDRC researchers, human and animal MRI facilities and the technical support needed to initiate and complete any imaging analysis. The Structural and Functional Visualization Core also works to develop new technologies for visualizing biological samples and in turn provide them to IDDRC researchers. In this proposal, we are focused on [1] developing smaller lighter one-photon miniaturized fluorescent microscopes for live imaging neural activity in freely moving animals and [2] refining the methods for CLARITY and iDISCO, protocols that render tissue transparent thereby permitting unparalleled visual acuity into the complex circuitry of the brain. These techniques offer the promise of a holistic approach to imaging, permitting IDDRC researchers to translate mechanism into therapy. For example, researchers investigating a specific intellectual disorder will be able perform MRI on patients to identify the affected region of the brain, implant miniaturized microscopes in rodent models to perform Ca2+ imaging in vivo to examine how the firing patterns of specific populations of neuron are mechanistically altered by the disease, while concomitantly examining putative aberrant circuit formation using light microscopy coupled with CLARITY. Finally, this core also supports the efforts of all the other cores, offering IDDRC researchers the ability to both probe molecular and cellular function at any level from the sub- cellular to living animals and determine the consequence of therapeutic interventions.