UCLA IDDRC: Structural and Functional Visualization Core

加州大学洛杉矶分校 IDDRC：结构和功能可视化核心

• 批准号: 10224913
• 负责人: SAMANTHA J BUTLER
• 金额: $ 18.45万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10224913
• 关键词: 3-Dimensional; Affect; Animal Model; Animals; Axon; Biological; Biological Models; Biological Process; Brain; Brain region; Cell Culture Techniques; Cell physiology; Cells; Complex; Coupled; Data; Dendrites; Developmental Disabilities; Disease; Fluorescence Microscopy; Gene Expression Profiling; Generations; Genetic; Human; Image; In Vitro; Intellectual and Developmental Disabilities Research Centers; Laboratories; Learning; Light; Light Microscope; Magnetic Resonance Imaging; Memory; Methodology; Methods; Microscope; Microscopy; Modality; Modeling; Molecular Probes; Mus; Nervous System Physiology; Neurons; Optics; Organ; Organoids; Patients; Pattern; Photons; Population; Protocols documentation; Recording of previous events; Research; Research Personnel; Research Support; Resolution; Resources; Rodent; Rodent Model; Role; Sampling; Scientist; Services; Sleep disturbances; Spectrum Analysis; Stem Cell Research; Structure; Techniques; Technology; Therapeutic Intervention; Tissues; Translating; Visual Acuity; Visualization; Work; animal imaging; brain circuitry; cognitive neuroscience; community center; developmental disease; fluorescence microscope; holistic approach; improved; in vivo imaging; light microscopy; member; miniaturize; nervous system development; neural circuit; neural implant; new technology; next generation; novel; novel imaging technology; open source; optical imaging; optogenetics; relating to nervous system; stem cells; submicron; two-photon; voucher

CORE E: STRUCTURAL AND FUNCTIONAL VISUALIZATION Samantha Butler, Core Director; Peyman Golshani, Core Co-Director; Neil Harris and Susan Bookheimer; MRI sub-core directors Abstract The Structural and Functional Visualization Core provides comprehensive imaging services to the members of UCLA Intellectual & Developmental Disabilities Research Center (IDDRC), working on any aspect of the genetic and environmentally-induced developmental diseases affecting nervous system development and function. The research ongoing in the UCLA IDDRC spans basic scientists using reductionist approaches to elucidate the mechanisms specific to intellectual developmental disabilities (IDDs) to clinicians assessing therapeutic interventions for patients. To accommodate all of their imaging requirements, we provide access to three light microscopy cores, including a microscopy suite dedicated to IDDRC researchers, human and animal MRI facilities and the technical support needed to initiate and complete any imaging analysis. The Structural and Functional Visualization Core also works to develop new technologies for visualizing biological samples and in turn provide them to IDDRC researchers. In this proposal, we are focused on [1] developing smaller lighter one-photon miniaturized fluorescent microscopes for live imaging neural activity in freely moving animals and [2] refining the methods for CLARITY and iDISCO, protocols that render tissue transparent thereby permitting unparalleled visual acuity into the complex circuitry of the brain. These techniques offer the promise of a holistic approach to imaging, permitting IDDRC researchers to translate mechanism into therapy. For example, researchers investigating a specific intellectual disorder will be able perform MRI on patients to identify the affected region of the brain, implant miniaturized microscopes in rodent models to perform Ca2+ imaging in vivo to examine how the firing patterns of specific populations of neuron are mechanistically altered by the disease, while concomitantly examining putative aberrant circuit formation using light microscopy coupled with CLARITY. Finally, this core also supports the efforts of all the other cores, offering IDDRC researchers the ability to both probe molecular and cellular function at any level from the sub- cellular to living animals and determine the consequence of therapeutic interventions.

核心 E：结构和功能可视化 萨曼莎·巴特勒，核心总监； Peyman Golshani，核心联合总监； 尼尔·哈里斯和苏珊·布克海默； MRI 子核心主任 抽象的 结构和功能可视化核心为 加州大学洛杉矶分校智力与发育障碍研究中心 (IDDRC) 成员，正在工作 影响遗传和环境诱发的发育疾病的任何方面 神经系统的发育和功能。加州大学洛杉矶分校 IDDRC 正在进行的研究涵盖 基础科学家使用还原论方法来阐明智力的特定机制 发育障碍 (IDD) 供临床医生评估患者的治疗干预措施。到 满足他们所有的成像要求，我们提供三光学显微镜 核心，包括专用于 IDDRC 研究人员、人类和动物 MRI 的显微镜套件 启动和完成任何成像分析所需的设施和技术支持。 结构和功能可视化核心还致力于开发新技术 用于可视化生物样本并将其提供给 IDDRC 研究人员。在这个提案中， 我们专注于 [1] 开发更小、更轻的单光子微型荧光显微镜 用于自由活动动物的实时成像神经活动并 [2] 改进 CLARITY 方法 和 iDISCO，使组织透明的协议，从而实现无与伦比的视敏度 进入大脑的复杂电路。这些技术提供了整体方法的希望 成像，使 IDDRC 研究人员能够将机制转化为治疗。例如， 调查特定智力障碍的研究人员将能够对患者进行核磁共振检查 识别受影响的大脑区域，在啮齿动物模型中植入微型显微镜 进行体内 Ca2+ 成像，以检查特定神经元群体的放电模式 被疾病机械地改变，同时检查假定的异常电路 使用光学显微镜结合 CLARITY 形成。 最后，该核心还支持所有其他核心的工作，提供 IDDRC 研究人员能够在任何水平上探测分子和细胞功能 细胞到活体动物并确定治疗干预的结果。