Animal and Phenotyping Core

动物和表型核心 项目摘要 杰克逊实验室内森休克中心（JAX NSC）提供多样化的动物资源，以提高 研究衰老的遗传学。动物和表型核心的总体目标是增加动物的多样性， 可用于衰老研究的老鼠资源。JAX NSC仍然是卓越的老龄化研究中心 用于开发和传播衰老小鼠模型和资源。这些资源包括32 JAX NSC免费提供全面的寿命和健康寿命数据的常见近交系， 交互式小鼠表型数据库（MPD）。在当前的资助期内，JAX研究人员建立了 使用高度多样化的品系（包括协作杂交（CC）近交系）研究衰老遗传学的模型 品系和多样性远交（DO）小鼠。除了动物资源外，理想情况下， 适合于大规模的，非侵入性的测试老化小鼠的开发。动物和表型核心将 继续为老龄化社区提供有价值的、高需求的动物和表型资源， 有效分配这些资源以确保其广泛使用。核心还将促进 通过与所有其他JAX NSC核心的广泛交互，实现NIA内森冲击中心计划的目标， 与其他NSC和NSC协调中心（NSCCC），并与整个老龄化社区。的 动物和表型核心的具体目标是：目标1。提供独特的动物资源， 老化研究具体地，在目标1A中，将连续地对DO、CC和C57 BL/6 J小鼠的老化集落进行处理。 保持“在货架上”，并可用于试点奖。在目标1B中，结合数据和统计 饮食干预研究的核心、寿命、广泛健康期表型和QTL分析 将完成上一个供资期间启动的项目。使动物资源的特性， 提供了与人类寿命和健康寿命相关的稳健和新颖的表型分析。价格实惠， 新的，有针对性的表型，组织和血液样本将提供给研究人员在老化领域， 有助于老年小鼠的全面表征。目标3：开展一项大规模的研究， 遗传多样性对DO小鼠衰老的影响以及抗衰老药物的作用 对潜在遗传学的反应。衰老和抗衰老剂的作用仅在 C57 BL/6 J小鼠。雄性和雌性DO对照小鼠和用衰老清除剂ABT 263处理的小鼠将被广泛地 表型分析，以便在各种组织和表型反应中将衰老和衰老清除处理偶联 在24个月大的时候就已经有了潜在的基因。目标4。与数据和统计核心协调， 通过JAX NSC网站分析数据并简化向老龄社区的传播工作， MPD。动物和表型核心将通过继续产生和分发 为老龄化研究界提供了宝贵的、高需求的小鼠遗传和表型资源。

ANIMAL AND PHENOTYPING CORE PROJECT SUMMARY The Jackson Laboratory Nathan Shock Center (JAX NSC) provides diverse animal resources to enhance research in the genetics of aging. The overall goal of the Animal and Phenotyping Core is to increase the diversity of mouse resources available for aging research. The JAX NSC remains the preeminent aging research center for the development and dissemination of aging mouse models and resources. These resources include 32 common inbred strains for which the JAX NSC freely provides comprehensive lifespan and healthspan data via the interactive Mouse Phenome Database (MPD). In the current funding period, JAX investigators established models to study the genetics of aging using highly diverse strains, including Collaborative Cross (CC) inbred strains and Diversity Outbred (DO) mice. In addition to animal resources, a robust phenotyping pipeline ideally suited for large-scale, non-invasive testing of aging mice was developed. The Animal and Phenotyping Core will continue to generate valuable, high-demand animals and phenotyping resources for the aging community and distribute these resources effectively to ensure their widespread use. The Core will also promote the overarching goals of the NIA Nathan Shock Center Program through extensive interactions with all other JAX NSC cores, with other NSCs and the NSC Coordinating Center (NSCCC), and with the aging community at large. The Specific Aims of the Animal and Phenotyping Core are to: Aim 1. Provide unique animal resources to support aging research. Specifically, in Aim 1A, aging colonies of DO, CC, and C57BL/6J mice will be continuously maintained “on the shelf” and available for Pilot Awards. In Aim 1B, in conjunction with the Data and Statistical Core, the lifespan, extensive healthspan phenotyping, and QTL analyses of the dietary intervention study initiated in the prior funding period will be completed.Aim 2. Enable characterization of animal resources by providing robust and novel phenotyping assays relevant to human lifespan and healthspan. Affordable, novel, targeted phenotyping, and tissue and blood samples will be provided to researchers in the aging field to facilitate a comprehensive characterization of aged mice. Aim 3. Implement a large-scale study on the impact of genetic diversity on senescence and the effect of senolytics in DO mice and couple phenotypic responses to the underlying genetics. Senescence and the effect of senolytics have only been studied in C57BL/6J mice. Male and female DO control mice and mice treated with senolytic ABT263 will be extensively phenotyped in order to couple senescence and senolytic treatment in various tissues and phenotypic responses at 24 months of age to the underlying genetics. Aim 4. Coordinate with the Data and Statistical Core to analyze data and streamline dissemination efforts to the aging community via the JAX NSC website and the MPD. The Animal and Phenotyping Core will support aging research by continuing to generate and distribute valuable, high-demand mouse genetic and phenotyping resources for the aging research community.