Animal and Phenotyping Core

动物和表型核心

• 批准号: 10045027
• 负责人: LUANNE L PETERS
• 金额: $ 59.28万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10045027
• 关键词: Age-Months; Aging; Animals; Anxiety; Award; Behavioral; Biological Assay; Biology of Aging; Biometry; Blood specimen; Chromosome Mapping; Clinical; Clinical Research; Communities; Complex; Data; Databases; Development; Dietary Intervention; Ensure; Equilibrium; Female; Funding; Genetic; Genetic Variation; Genetic study; Goals; Hand Strength; Hematology; Human; Immune; Inbred Strain; Inbreeding; Intervention; Intervention Studies; Kidney; Laboratories; Longevity; Methods; Modeling; Mus; Organ; Phenotype; Pilot Projects; Population; Process; Quality Control; Quantitative Trait Loci; Reproducibility; Research; Research Personnel; Resolution; Resources; Shock; Testing; The Jackson Laboratory; Time; Tissue Sample; Tissues; Work; aged; animal resource; behavioral health; cohort; genetic analysis; healthspan; male; mouse genetics; mouse model; novel; outreach; phenome; physical conditioning; programs; research and development; response; senescence; trend; web site

ANIMAL AND PHENOTYPING CORE PROJECT SUMMARY The Jackson Laboratory Nathan Shock Center (JAX NSC) provides diverse animal resources to enhance research in the genetics of aging. The overall goal of the Animal and Phenotyping Core is to increase the diversity of mouse resources available for aging research. The JAX NSC remains the preeminent aging research center for the development and dissemination of aging mouse models and resources. These resources include 32 common inbred strains for which the JAX NSC freely provides comprehensive lifespan and healthspan data via the interactive Mouse Phenome Database (MPD). In the current funding period, JAX investigators established models to study the genetics of aging using highly diverse strains, including Collaborative Cross (CC) inbred strains and Diversity Outbred (DO) mice. In addition to animal resources, a robust phenotyping pipeline ideally suited for large-scale, non-invasive testing of aging mice was developed. The Animal and Phenotyping Core will continue to generate valuable, high-demand animals and phenotyping resources for the aging community and distribute these resources effectively to ensure their widespread use. The Core will also promote the overarching goals of the NIA Nathan Shock Center Program through extensive interactions with all other JAX NSC cores, with other NSCs and the NSC Coordinating Center (NSCCC), and with the aging community at large. The Specific Aims of the Animal and Phenotyping Core are to: Aim 1. Provide unique animal resources to support aging research. Specifically, in Aim 1A, aging colonies of DO, CC, and C57BL/6J mice will be continuously maintained “on the shelf” and available for Pilot Awards. In Aim 1B, in conjunction with the Data and Statistical Core, the lifespan, extensive healthspan phenotyping, and QTL analyses of the dietary intervention study initiated in the prior funding period will be completed.Aim 2. Enable characterization of animal resources by providing robust and novel phenotyping assays relevant to human lifespan and healthspan. Affordable, novel, targeted phenotyping, and tissue and blood samples will be provided to researchers in the aging field to facilitate a comprehensive characterization of aged mice. Aim 3. Implement a large-scale study on the impact of genetic diversity on senescence and the effect of senolytics in DO mice and couple phenotypic responses to the underlying genetics. Senescence and the effect of senolytics have only been studied in C57BL/6J mice. Male and female DO control mice and mice treated with senolytic ABT263 will be extensively phenotyped in order to couple senescence and senolytic treatment in various tissues and phenotypic responses at 24 months of age to the underlying genetics. Aim 4. Coordinate with the Data and Statistical Core to analyze data and streamline dissemination efforts to the aging community via the JAX NSC website and the MPD. The Animal and Phenotyping Core will support aging research by continuing to generate and distribute valuable, high-demand mouse genetic and phenotyping resources for the aging research community.

动物和表型核心 项目总结 杰克逊实验室内森休克中心(JAX NSC)提供多样化的动物资源，以增强 衰老遗传学方面的研究。动物和表型核心的总体目标是增加多样性 可用于衰老研究的鼠标资源。JAX NSC仍然是卓越的老龄化研究中心 用于开发和传播老化小鼠模型和资源。这些资源包括32个 JAX NSC通过以下方式免费提供全面寿命和健康范围数据的常见近交系 交互式鼠标表现组数据库(MPD)。在本供资期间，日本宇宙航空研究开发机构的调查人员建立了 使用高度不同的品系研究衰老遗传学的模型，包括合作杂交(CC)近交系 品系和多样性杂交(DO)小鼠。除了动物资源，理想情况下，一个强大的表型鉴定管道 开发了一种适用于大规模、非侵入性的衰老小鼠测试的新技术。动物和表型核心意志 继续为老龄化社区创造有价值的、高需求的动物和表型资源，以及 有效地分配这些资源，以确保其广泛使用。核心还将促进总体 通过与所有其他JAX NSC核心的广泛互动，NIA Nathan Shock Center计划的目标， 与其他NSC和NSC协调中心(NSCCC)以及整个老龄化社区的关系。这个 动物和表型核心的具体目标是：目标1.提供独特的动物资源以支持 老龄化研究。具体地说，在目标1A中，DO、CC和C57BL/6J小鼠的老化群体将持续 保持“搁置”状态，并可用于飞行员奖。在Aim 1B中，结合数据和统计 膳食干预研究的核心、寿命、广泛健康范围表型和QTL分析 将完成在前一个供资期间启动的工作。目标2.通过以下方式确定动物资源的特性 提供与人类寿命和健康相关的可靠和新颖的表型分析。价格实惠， 将向老龄化领域的研究人员提供新的、有针对性的表型鉴定以及组织和血液样本，以 促进对衰老小鼠的全面描述。目标3.实施关于影响的大规模研究 遗传多样性对DO小鼠衰老的影响及增老剂的影响 对潜在遗传学的反应。衰老和衰老剂的作用仅在 C57BL/6J小鼠。雄性和雌性DO对照组小鼠和用感觉剂ABT263处理的小鼠将被广泛 在不同组织中结合衰老和衰老处理的表型和表型反应 在24个月大的时候对潜在的遗传学产生了影响。目标4.与数据和统计核心协调，以 通过JAX NSC网站分析数据并简化向老龄化社区的传播工作 公安部。动物和表型核心将通过继续生成和分发来支持衰老研究 为老龄化研究社区提供有价值的、高需求的小鼠遗传和表型资源。