Cytek Aurora Full Spectrum Flow Cytometer

Cytek Aurora 全谱流式细胞仪

• 批准号: 10429777
• 负责人: Yoav Altman
• 金额: $ 46.83万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-20 至 2024-06-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10429777
• 关键词: Accounting; Aging; Biological Process; Biomedical Research; California; Cells; Child; Childhood Medulloblastomas; Color; Coronavirus; Cytometry; Developmental Biology; Disease; Flow Cytometry; Fluorescence; Funding; Genomics; Gold; Gut Mucosa; Health; Healthcare; Heterogeneity; Immune; Immune Evasion; Immunooncology; Infection; Inflammation; Inflammatory; Institutes; Lasers; Malignant Neoplasms; Malignant neoplasm of pancreas; Measures; Medical; Molecular; Mucosal Immunity; Proteomics; Protocols documentation; Publications; Research Institute; Research Personnel; Running; Sampling; T-Lymphocyte; Technology; Time; Tissues; Tumor Immunity; United States National Institutes of Health; Universities; base; exhaustion; graft vs host disease; high standard; insight; malignant breast neoplasm; melanoma; single cell analysis; time use

PROJECT SUMMARY Sanford Burnham Prebys Medical Discovery Institute (SBP) has ~40 labs studying the molecular and cellular underpinnings of diverse biological processes including cancer; developmental biology; immuno-oncology; infection, inflammation; aging and related diseases. Many recent advances in health care depend upon new insights into tissue heterogeneity at the single cell level. Flow cytometry is the gold standard for high throughput single cell analysis, however conventional flow is limited by the number of analytes that can be run in unison. Spectral flow cytometers recently achieved the parameter depth required for deep immune profiling with publications showing 40+ color rivaling time-of-flight mass cytometry (CyTOF) parameter depth with ~50-fold higher throughput. SBP requests support for a Cytek Aurora Spectral Flow Cytometer with 5 lasers (UV/V/B/YG/R), 64 fluorescence and 3 scattering channels, and Automatic Micro-sampling Station (AMS) to enable high- parameter single-cell proteomic analysis for deep profiling of cell heterogeneity in tissues. We also request support for a BioBUBBLE Class I Negative Biocontainment Enclosure for Cytek Aurora to protect researchers running potentially infectious material. The Aurora more than doubles the number of fluorescent parameters that can be measured at SBP and leverages existing FACS protocols and expertise. It is a bridging technology between high-throughput low-plex conventional flow cytometers in SBP’s Core and high-plex low- throughput genomics-based single-cell Seq technologies. This application is supported by a coalition of 12 Major Users from SBP and nearby University of California San Diego (UCSD) and San Diego Biomedical Research Institute (SDBRI) demonstrating the broad impact for the region. NIH-funded researchers, together accounting for 75% of the Aurora’s available use time (AUT), need the Aurora to advance their understanding of T cell exhaustion, anti-tumor immunity, aging, multi-system inflammatory disease in children (MIS-C), gut mucosal immunity, melanoma, pancreatic cancer, breast cancer, pediatric medulloblastoma, graft versus host disease, and the immune evasion tactics of Coronavirus. The Aurora will provide unprecedented insight into tissue signatures that correlate with health and disease leading to new mechanistic inquiries and discoveries.

项目概要 桑福德伯纳姆普雷比斯医学发现研究所 (SBP) 拥有约 40 个研究分子和细胞的实验室 包括癌症在内的多种生物过程的基础；发育生物学；免疫肿瘤学； 感染、炎症；衰老和相关疾病。医疗保健领域的许多最新进展都依赖于新的 在单细胞水平上洞察组织异质性。流式细胞术是高通量的黄金标准 单细胞分析，然而传统流程受到可同时运行的分析物数量的限制。 光谱流式细胞仪最近达到了深度免疫分析所需的参数深度 出版物显示 40 多种颜色可与飞行时间质谱流式细胞仪 (CyTOF) 参数深度相媲美，约为 50 倍 更高的吞吐量。 SBP 请求支持配备 5 个激光器（UV/V/B/YG/R）的 Cytek Aurora 光谱流式细胞仪，64 荧光和 3 个散射通道，以及自动微量采样站 (AMS)，以实现高 参数单细胞蛋白质组分析，用于深入分析组织中细胞异质性。我们还要求 支持 Cytek Aurora 的 BioBUBBLE I 级负生物防护外壳以提供保护 研究人员正在运行具有潜在感染性的材料。 Aurora 的荧光灯数量增加了一倍多 可以在 SBP 上测量的参数，并利用现有的 FACS 协议和专业知识。它是一个桥梁 SBP 核心中的高通量低复数传统流式细胞仪与高复数低复数流式细胞仪之间的技术 基于通量基因组学的单细胞测序技术。 该应用程序由来自 SBP 和附近的加州大学圣保罗分校的 12 个主要用户组成的联盟支持 圣地亚哥 (UCSD) 和圣地亚哥生物医学研究所 (SDBRI) 展示了对 地区。 NIH 资助的研究人员合计占 Aurora 可用时间 (AUT) 的 75%，他们需要 Aurora 促进他们对 T 细胞耗竭、抗肿瘤免疫、衰老、多系统的理解 儿童炎症性疾病（MIS-C）、肠道粘膜免疫、黑色素瘤、胰腺癌、乳腺癌、 儿科髓母细胞瘤、移植物抗宿主病以及冠状病毒的免疫逃避策略。这 Aurora 将提供对与健康和疾病相关的组织特征的前所未有的洞察，从而导致 新的机制探究和发现。