Cytek Aurora Full Spectrum Flow Cytometer

Cytek Aurora 全谱流式细胞仪

• 批准号: 10429777
• 负责人: Yoav Altman
• 金额: $ 46.83万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-20 至 2024-06-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10429777
• 关键词: Accounting; Aging; Biological Process; Biomedical Research; California; Cells; Child; Childhood Medulloblastomas; Color; Coronavirus; Cytometry; Developmental Biology; Disease; Flow Cytometry; Fluorescence; Funding; Genomics; Gold; Gut Mucosa; Health; Healthcare; Heterogeneity; Immune; Immune Evasion; Immunooncology; Infection; Inflammation; Inflammatory; Institutes; Lasers; Malignant Neoplasms; Malignant neoplasm of pancreas; Measures; Medical; Molecular; Mucosal Immunity; Proteomics; Protocols documentation; Publications; Research Institute; Research Personnel; Running; Sampling; T-Lymphocyte; Technology; Time; Tissues; Tumor Immunity; United States National Institutes of Health; Universities; base; exhaustion; graft vs host disease; high standard; insight; malignant breast neoplasm; melanoma; single cell analysis; time use

PROJECT SUMMARY Sanford Burnham Prebys Medical Discovery Institute (SBP) has ~40 labs studying the molecular and cellular underpinnings of diverse biological processes including cancer; developmental biology; immuno-oncology; infection, inflammation; aging and related diseases. Many recent advances in health care depend upon new insights into tissue heterogeneity at the single cell level. Flow cytometry is the gold standard for high throughput single cell analysis, however conventional flow is limited by the number of analytes that can be run in unison. Spectral flow cytometers recently achieved the parameter depth required for deep immune profiling with publications showing 40+ color rivaling time-of-flight mass cytometry (CyTOF) parameter depth with ~50-fold higher throughput. SBP requests support for a Cytek Aurora Spectral Flow Cytometer with 5 lasers (UV/V/B/YG/R), 64 fluorescence and 3 scattering channels, and Automatic Micro-sampling Station (AMS) to enable high- parameter single-cell proteomic analysis for deep profiling of cell heterogeneity in tissues. We also request support for a BioBUBBLE Class I Negative Biocontainment Enclosure for Cytek Aurora to protect researchers running potentially infectious material. The Aurora more than doubles the number of fluorescent parameters that can be measured at SBP and leverages existing FACS protocols and expertise. It is a bridging technology between high-throughput low-plex conventional flow cytometers in SBP’s Core and high-plex low- throughput genomics-based single-cell Seq technologies. This application is supported by a coalition of 12 Major Users from SBP and nearby University of California San Diego (UCSD) and San Diego Biomedical Research Institute (SDBRI) demonstrating the broad impact for the region. NIH-funded researchers, together accounting for 75% of the Aurora’s available use time (AUT), need the Aurora to advance their understanding of T cell exhaustion, anti-tumor immunity, aging, multi-system inflammatory disease in children (MIS-C), gut mucosal immunity, melanoma, pancreatic cancer, breast cancer, pediatric medulloblastoma, graft versus host disease, and the immune evasion tactics of Coronavirus. The Aurora will provide unprecedented insight into tissue signatures that correlate with health and disease leading to new mechanistic inquiries and discoveries.

项目总结 桑福德·伯纳姆·普雷比医学发现研究所(SBP)有大约40个实验室研究分子和细胞 包括癌症在内的多种生物学过程的基础；发育生物学；免疫肿瘤学； 感染、炎症、衰老和相关疾病。医疗保健方面的许多最新进展依赖于新的 在单细胞水平上对组织异质性的洞察。流式细胞术是高通量的金标准 单细胞分析，然而，传统的流程受到可同时运行的分析物数量的限制。 光谱流式细胞仪最近达到了深度免疫分析所需的参数深度 出版物展示了40多种颜色，可与飞行时间质量细胞术(CyTOF)参数深度相媲美，达到约50倍 更高的吞吐量。 SBP请求支持带有5个激光(UV/V/B/YG/R)的Cytek Aurora光谱流式细胞仪， 荧光和3个散射通道，以及自动微量采样站(AMS)，以实现高 组织中细胞异质性深度剖析的参数单细胞蛋白质组学分析。我们还要求 支持为Cytek Aurora提供保护的BioBUBBLE I类负生物包容外壳 研究人员正在运行可能具有传染性的材料。极光使荧光灯的数量增加了一倍多 可在SBP测量的参数，并利用现有的FACS协议和专业知识。这是一座桥梁 SBP核心高通量低复用常规流量计与高复用低功耗流量计之间的技术 以基因组学为基础的单细胞序列技术。 该应用程序由来自SBP和附近的加州大学旧金山分校的12个主要用户组成的联盟支持 迭戈(UCSD)和圣地亚哥生物医学研究所(SDBRI)展示了 区域。美国国立卫生研究院资助的研究人员，加起来占极光可用使用时间(AUT)的75%，需要 极光促进他们对T细胞耗竭、抗肿瘤免疫、衰老、多系统的认识 儿童炎症性疾病(MIS-C)、肠道粘膜免疫、黑色素瘤、胰腺癌、乳腺癌 儿童髓母细胞瘤、移植物抗宿主病和冠状病毒的免疫逃避策略。这个 极光将提供前所未有的对与健康和疾病相关的组织特征的洞察，从而导致 新的机械探索和发现。