Cytek Aurora Full Spectrum Flow Cytometer
Cytek Aurora 全谱流式细胞仪
基本信息
- 批准号:10429777
- 负责人:
- 金额:$ 46.83万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-09-20 至 2024-06-19
- 项目状态:已结题
- 来源:
- 关键词:AccountingAgingBiological ProcessBiomedical ResearchCaliforniaCellsChildChildhood MedulloblastomasColorCoronavirusCytometryDevelopmental BiologyDiseaseFlow CytometryFluorescenceFundingGenomicsGoldGut MucosaHealthHealthcareHeterogeneityImmuneImmune EvasionImmunooncologyInfectionInflammationInflammatoryInstitutesLasersMalignant NeoplasmsMalignant neoplasm of pancreasMeasuresMedicalMolecularMucosal ImmunityProteomicsProtocols documentationPublicationsResearch InstituteResearch PersonnelRunningSamplingT-LymphocyteTechnologyTimeTissuesTumor ImmunityUnited States National Institutes of HealthUniversitiesbaseexhaustiongraft vs host diseasehigh standardinsightmalignant breast neoplasmmelanomasingle cell analysistime use
项目摘要
PROJECT SUMMARY
Sanford Burnham Prebys Medical Discovery Institute (SBP) has ~40 labs studying the molecular and cellular
underpinnings of diverse biological processes including cancer; developmental biology; immuno-oncology;
infection, inflammation; aging and related diseases. Many recent advances in health care depend upon new
insights into tissue heterogeneity at the single cell level. Flow cytometry is the gold standard for high throughput
single cell analysis, however conventional flow is limited by the number of analytes that can be run in unison.
Spectral flow cytometers recently achieved the parameter depth required for deep immune profiling with
publications showing 40+ color rivaling time-of-flight mass cytometry (CyTOF) parameter depth with ~50-fold
higher throughput.
SBP requests support for a Cytek Aurora Spectral Flow Cytometer with 5 lasers (UV/V/B/YG/R), 64
fluorescence and 3 scattering channels, and Automatic Micro-sampling Station (AMS) to enable high-
parameter single-cell proteomic analysis for deep profiling of cell heterogeneity in tissues. We also request
support for a BioBUBBLE Class I Negative Biocontainment Enclosure for Cytek Aurora to protect
researchers running potentially infectious material. The Aurora more than doubles the number of fluorescent
parameters that can be measured at SBP and leverages existing FACS protocols and expertise. It is a bridging
technology between high-throughput low-plex conventional flow cytometers in SBP’s Core and high-plex low-
throughput genomics-based single-cell Seq technologies.
This application is supported by a coalition of 12 Major Users from SBP and nearby University of California San
Diego (UCSD) and San Diego Biomedical Research Institute (SDBRI) demonstrating the broad impact for the
region. NIH-funded researchers, together accounting for 75% of the Aurora’s available use time (AUT), need the
Aurora to advance their understanding of T cell exhaustion, anti-tumor immunity, aging, multi-system
inflammatory disease in children (MIS-C), gut mucosal immunity, melanoma, pancreatic cancer, breast cancer,
pediatric medulloblastoma, graft versus host disease, and the immune evasion tactics of Coronavirus. The
Aurora will provide unprecedented insight into tissue signatures that correlate with health and disease leading to
new mechanistic inquiries and discoveries.
项目概要
桑福德伯纳姆普雷比斯医学发现研究所 (SBP) 拥有约 40 个研究分子和细胞的实验室
包括癌症在内的多种生物过程的基础;发育生物学;免疫肿瘤学;
感染、炎症;衰老和相关疾病。医疗保健领域的许多最新进展都依赖于新的
在单细胞水平上洞察组织异质性。流式细胞术是高通量的黄金标准
单细胞分析,然而传统流程受到可同时运行的分析物数量的限制。
光谱流式细胞仪最近达到了深度免疫分析所需的参数深度
出版物显示 40 多种颜色可与飞行时间质谱流式细胞仪 (CyTOF) 参数深度相媲美,约为 50 倍
更高的吞吐量。
SBP 请求支持配备 5 个激光器(UV/V/B/YG/R)的 Cytek Aurora 光谱流式细胞仪,64
荧光和 3 个散射通道,以及自动微量采样站 (AMS),以实现高
参数单细胞蛋白质组分析,用于深入分析组织中细胞异质性。我们还要求
支持 Cytek Aurora 的 BioBUBBLE I 级负生物防护外壳以提供保护
研究人员正在运行具有潜在感染性的材料。 Aurora 的荧光灯数量增加了一倍多
可以在 SBP 上测量的参数,并利用现有的 FACS 协议和专业知识。它是一个桥梁
SBP 核心中的高通量低复数传统流式细胞仪与高复数低复数流式细胞仪之间的技术
基于通量基因组学的单细胞测序技术。
该应用程序由来自 SBP 和附近的加州大学圣保罗分校的 12 个主要用户组成的联盟支持
圣地亚哥 (UCSD) 和圣地亚哥生物医学研究所 (SDBRI) 展示了对
地区。 NIH 资助的研究人员合计占 Aurora 可用时间 (AUT) 的 75%,他们需要
Aurora 促进他们对 T 细胞耗竭、抗肿瘤免疫、衰老、多系统的理解
儿童炎症性疾病(MIS-C)、肠道粘膜免疫、黑色素瘤、胰腺癌、乳腺癌、
儿科髓母细胞瘤、移植物抗宿主病以及冠状病毒的免疫逃避策略。这
Aurora 将提供对与健康和疾病相关的组织特征的前所未有的洞察,从而导致
新的机制探究和发现。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Yoav Altman其他文献
Yoav Altman的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
相似海外基金
Interplay between Aging and Tubulin Posttranslational Modifications
衰老与微管蛋白翻译后修饰之间的相互作用
- 批准号:
24K18114 - 财政年份:2024
- 资助金额:
$ 46.83万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
The Canadian Brain Health and Cognitive Impairment in Aging Knowledge Mobilization Hub: Sharing Stories of Research
加拿大大脑健康和老龄化认知障碍知识动员中心:分享研究故事
- 批准号:
498288 - 财政年份:2024
- 资助金额:
$ 46.83万 - 项目类别:
Operating Grants
EMNANDI: Advanced Characterisation and Aging of Compostable Bioplastics for Automotive Applications
EMNANDI:汽车应用可堆肥生物塑料的高级表征和老化
- 批准号:
10089306 - 财政年份:2024
- 资助金额:
$ 46.83万 - 项目类别:
Collaborative R&D
関節リウマチ患者のSuccessful Agingに向けたフレイル予防対策の構築
类风湿性关节炎患者成功老龄化的衰弱预防措施的建立
- 批准号:
23K20339 - 财政年份:2024
- 资助金额:
$ 46.83万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Baycrest Academy for Research and Education Summer Program in Aging (SPA): Strengthening research competencies, cultivating empathy, building interprofessional networks and skills, and fostering innovation among the next generation of healthcare workers t
Baycrest Academy for Research and Education Summer Program in Aging (SPA):加强研究能力,培养同理心,建立跨专业网络和技能,并促进下一代医疗保健工作者的创新
- 批准号:
498310 - 财政年份:2024
- 资助金额:
$ 46.83万 - 项目类别:
Operating Grants
Life course pathways in healthy aging and wellbeing
健康老龄化和福祉的生命历程路径
- 批准号:
2740736 - 财政年份:2024
- 资助金额:
$ 46.83万 - 项目类别:
Studentship
I-Corps: Aging in Place with Artificial Intelligence-Powered Augmented Reality
I-Corps:利用人工智能驱动的增强现实实现原地老龄化
- 批准号:
2406592 - 财政年份:2024
- 资助金额:
$ 46.83万 - 项目类别:
Standard Grant
NSF PRFB FY 2023: Connecting physiological and cellular aging to individual quality in a long-lived free-living mammal.
NSF PRFB 2023 财年:将生理和细胞衰老与长寿自由生活哺乳动物的个体质量联系起来。
- 批准号:
2305890 - 财政年份:2024
- 资助金额:
$ 46.83万 - 项目类别:
Fellowship Award
虚弱高齢者のSuccessful Agingを支える地域課題分析指標と手法の確立
建立区域问题分析指标和方法,支持体弱老年人成功老龄化
- 批准号:
23K20355 - 财政年份:2024
- 资助金额:
$ 46.83万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
「ケア期間」に着目したbiological aging指標の開発
开发聚焦“护理期”的生物衰老指数
- 批准号:
23K24782 - 财政年份:2024
- 资助金额:
$ 46.83万 - 项目类别:
Grant-in-Aid for Scientific Research (B)














{{item.name}}会员




