Core 2: Rodent Behavioral Core

项目摘要-核心2 酗酒的易感性有明显的遗传因素。VCU-ARC及其合作者寻求更好地 通过研究跨物种遗传相关性来了解乙醇反应基因。这些努力 产生了几个乙醇响应基因候选者，如Clic 4，GSK 3B和slo 1。啮齿动物 行为核心（核心2）将通过研究操纵这些（和 新发现的）啮齿动物行为电池中的候选基因，该电池模拟急性乙醇的特定方面 敏感性、消费和寻求行为。核心2将使用条件转基因小鼠或病毒 转基因递送至小鼠或大鼠脑。核心2也可能使用环境调节来研究x基因 环境交互作用对乙醇响应行为的影响。核心蛋白2的目的是将表达水平 乙醇反应候选基因对特定行为结果的影响，以获得更深层次的 了解酗酒的遗传基础。核心2的结果将提供有价值的见解， 复杂的行为和神经生物学适应的遗传基础， 急性或反复乙醇暴露。这些新知识将有助于制定干预措施， 预防和/或治疗酒精滥用和酒精中毒。

Project Summary – Core 2 Susceptibility to alcoholism has a clear genetic component. The VCU-ARC and its collaborators seeks to better understand ethanol-responsive genes by studying cross-species genetic correlates. These efforts have generated several ethanol-responsive gene candidates such as Clic4, GSK3B, and slo1. The Rodent Behavioral Core (Core 2) will expand these efforts by studying the consequences of manipulating these (and newly identified) candidate genes in a rodent behavioral battery that models specific aspects of acute ethanol sensitivity, consumption, and seeking behavior. Core 2 will use conditional genetically modified mice or viral transgene delivery to mouse or rat brain. Core 2 may also use environmental modulations to investigate gene x environment interactions on ethanol response behaviors. The intent of Core 2 is to correlate expression levels of an ethanol-responsive candidate gene to a particular behavioral outcome in order to gain a deeper understanding of the genetic basis of alcoholism. The results of Core 2 will provide valuable insight into the genetic basis of the complex behavioral and neurobiological adaptations that develop as a consequence of either acute or repeated ethanol exposure. This new knowledge will aid in the development of interventions for the prevention and/or treatment of ethanol abuse and alcoholism.