10X Chromium Connect for Single Cell Library Preparations for Translational Research

项目总结/摘要 要求资金购买10 X Genomics Chromium Connect，以满足当前和未来 Moores癌症中心（MCC）和医学院的许多研究人员的需求 加州大学圣地亚哥分校。所要求的10 X Genomics Chromium Connect是一款独一无二的机器人 该平台通过集成单细胞分区、条形码和 将RNA文库制备整合到一个优化的解决方案中，减少工作流程时间并降低阈值 用于进行单细胞分选和测序。单细胞测序正在迅速成为一种强大的工具 在基础，转化和临床癌症研究，以及在其他临床疾病的研究。的 使用单细胞技术获得的知识深度正在改变新疗法的速度， 癌症和其他疾病的治疗方法可以被开发和微调。10X Genomics Chromium Connect 平台简化了单细胞测序工作流程，降低了单细胞测序的门槛。 为所有用户进行分类和排序，特别是在与专家随时访问的情况下， 设计单细胞实验和分析生成的测序数据。该装置减少了 样品与样品之间的可变性，并避免人为移液错误，从而增加了 从运行到运行的测序数据收集。Chromium Connect的增强功能， 圣地亚哥现有的仪器将为单细胞测序提供更多的机会 我们的目标是为MCC用户提供先进的技术，从而促进癌症预防、诊断和护理。 我们的NIH资助用户研究各种项目，从肿瘤微环境的分析， 肿瘤免疫学、细胞信号传导、癌症干细胞生物学、癌症治疗学和癌症护理， 代表了癌症和癌症治疗研究的多种方法。这些项目跨越 基础、转化和临床癌症研究。此外，我们的一些MCC调查员 疾病生物学其他重要领域的研究。所有这些都有一个共同点，即高吞吐量、鲁棒的单 细胞测序工作流程将通过实现更可靠的单细胞测序运行来增强他们的研究， 通过消除将单一测序纳入其NIH资助研究的障碍。 Chromium Connect将在新的单细胞处理中定位、管理和维护 Moores癌症中心三楼的共享资源-一个共享资源，将提供自动化的 单细胞测序、用于活细胞测定的单细胞分选和数字空间分析--所有这些技术 是先进的癌症研究所必需的。Chromium Connect将参与MCC成本回收 该系统确保部分收回薪金、维修协议和用品的资金。

PROJECT SUMMARY/ABSTRACT Funds are requested to purchase a 10X Genomics Chromium Connect to meet the ongoing and future demands of numerous researchers within the Moores Cancer Center (MCC) and the School of Medicine at the University of California, San Diego. The requested 10X Genomics Chromium Connect is a one-of-a-kind robotic platform that automates single cell sequencing workflows by integrating single cell partitioning, barcoding, and RNA library preparation together into one optimized solution that reduces workflow time and lowers the threshold for performing single cell sorting and sequencing. Single cell sequencing is rapidly becoming a powerful tool in basic, translational, and clinical cancer research, as well as in the study of other clinical disorders. The depth of knowledge gained using single cell technologies is transforming the speed at which new therapies for cancer and other diseases can be developed and fine-tuned. The 10X Genomics Chromium Connect platform simplifies single cell sequencing workflows and lowers the threshold for performing single cell sorting and sequencing for all users, especially when coupled with ready access to experts in both the design of single cell experiments and the analysis of generated sequencing data. This device reduces sample to sample variability and avoids human pipetting errors, thus adding substantial reliability to sequencing data collection from run to run. The enhanced capabilities of the Chromium Connect over current instrumentation available in San Diego will provide greater access to single cell sequencing technologies to MCC users and thereby will advance cancer prevention, diagnosis, and care. Our NIH funded users study a variety of projects, ranging from analysis of the tumor microenvironment, tumor immunology, cell signaling, cancer stem cell biology, cancer therapeutics, and cancer care, representing a wide variety of approaches to the study of cancer and cancer therapy. These projects span basic, translational, and clinical cancer research. In addition, some of our MCC investigators conduct research in other significant areas of disease biology. All have in common that high throughput, robust single cell sequencing workflows will enhance their research by enabling more reliable single cell sequencing runs and by eliminating barriers to the incorporation of single sequencing into their NIH funded studies. The Chromium Connect will be located, administered, and maintained in the new Single Cell Processing Shared Resource on the third floor of the Moores Cancer Center – a shared resource that will offer automated single cell sequencing, single cell sorting for live cell assays, and digital spatial profiling - all technologies that are needed for advanced cancer research. The Chromium Connect will participate in the MCC cost recovery system to ensure partial recovery of funds for salaries, maintenance agreements, and supplies.