10X Chromium Connect for Single Cell Library Preparations for Translational Research

项目总结/文摘

PROJECT SUMMARY/ABSTRACT Funds are requested to purchase a 10X Genomics Chromium Connect to meet the ongoing and future demands of numerous researchers within the Moores Cancer Center (MCC) and the School of Medicine at the University of California, San Diego. The requested 10X Genomics Chromium Connect is a one-of-a-kind robotic platform that automates single cell sequencing workflows by integrating single cell partitioning, barcoding, and RNA library preparation together into one optimized solution that reduces workflow time and lowers the threshold for performing single cell sorting and sequencing. Single cell sequencing is rapidly becoming a powerful tool in basic, translational, and clinical cancer research, as well as in the study of other clinical disorders. The depth of knowledge gained using single cell technologies is transforming the speed at which new therapies for cancer and other diseases can be developed and fine-tuned. The 10X Genomics Chromium Connect platform simplifies single cell sequencing workflows and lowers the threshold for performing single cell sorting and sequencing for all users, especially when coupled with ready access to experts in both the design of single cell experiments and the analysis of generated sequencing data. This device reduces sample to sample variability and avoids human pipetting errors, thus adding substantial reliability to sequencing data collection from run to run. The enhanced capabilities of the Chromium Connect over current instrumentation available in San Diego will provide greater access to single cell sequencing technologies to MCC users and thereby will advance cancer prevention, diagnosis, and care. Our NIH funded users study a variety of projects, ranging from analysis of the tumor microenvironment, tumor immunology, cell signaling, cancer stem cell biology, cancer therapeutics, and cancer care, representing a wide variety of approaches to the study of cancer and cancer therapy. These projects span basic, translational, and clinical cancer research. In addition, some of our MCC investigators conduct research in other significant areas of disease biology. All have in common that high throughput, robust single cell sequencing workflows will enhance their research by enabling more reliable single cell sequencing runs and by eliminating barriers to the incorporation of single sequencing into their NIH funded studies. The Chromium Connect will be located, administered, and maintained in the new Single Cell Processing Shared Resource on the third floor of the Moores Cancer Center – a shared resource that will offer automated single cell sequencing, single cell sorting for live cell assays, and digital spatial profiling - all technologies that are needed for advanced cancer research. The Chromium Connect will participate in the MCC cost recovery system to ensure partial recovery of funds for salaries, maintenance agreements, and supplies.