10X Chromium Connect for Single Cell Library Preparations for Translational Research

用于转化研究的单细胞文库制备的 10X Chromium Connect

• 批准号: 10430292
• 负责人: Judith A VARNER
• 金额: $ 28.35万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-15 至 2023-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10430292
• 关键词: Advanced Malignant Neoplasm; Agreement; Area; Bar Codes; Biology; California; Cancer Center; Caring; Cell Separation; Cells; Cellular Assay; Chromium; Clinical; Coupled; Data; Data Collection; Devices; Diagnosis; Disease; Ensure; Floor; Funding; Future; Genomics; Human; Knowledge; Libraries; Maintenance; Malignant Neoplasms; Preparation; RNA library; Recovery; Research; Research Personnel; Resource Sharing; Robotics; Running; Sampling; Signal Transduction; Speed; System; Technology; Therapeutic; Time; Translational Research; United States National Institutes of Health; Universities; Wages; anticancer research; cancer care; cancer prevention; cancer stem cell; cancer therapy; cost; design; digital; experimental study; instrumentation; medical schools; novel therapeutics; partial recovery; single cell sequencing; single cell technology; stem cell biology; tool; tumor immunology; tumor microenvironment

PROJECT SUMMARY/ABSTRACT Funds are requested to purchase a 10X Genomics Chromium Connect to meet the ongoing and future demands of numerous researchers within the Moores Cancer Center (MCC) and the School of Medicine at the University of California, San Diego. The requested 10X Genomics Chromium Connect is a one-of-a-kind robotic platform that automates single cell sequencing workflows by integrating single cell partitioning, barcoding, and RNA library preparation together into one optimized solution that reduces workflow time and lowers the threshold for performing single cell sorting and sequencing. Single cell sequencing is rapidly becoming a powerful tool in basic, translational, and clinical cancer research, as well as in the study of other clinical disorders. The depth of knowledge gained using single cell technologies is transforming the speed at which new therapies for cancer and other diseases can be developed and fine-tuned. The 10X Genomics Chromium Connect platform simplifies single cell sequencing workflows and lowers the threshold for performing single cell sorting and sequencing for all users, especially when coupled with ready access to experts in both the design of single cell experiments and the analysis of generated sequencing data. This device reduces sample to sample variability and avoids human pipetting errors, thus adding substantial reliability to sequencing data collection from run to run. The enhanced capabilities of the Chromium Connect over current instrumentation available in San Diego will provide greater access to single cell sequencing technologies to MCC users and thereby will advance cancer prevention, diagnosis, and care. Our NIH funded users study a variety of projects, ranging from analysis of the tumor microenvironment, tumor immunology, cell signaling, cancer stem cell biology, cancer therapeutics, and cancer care, representing a wide variety of approaches to the study of cancer and cancer therapy. These projects span basic, translational, and clinical cancer research. In addition, some of our MCC investigators conduct research in other significant areas of disease biology. All have in common that high throughput, robust single cell sequencing workflows will enhance their research by enabling more reliable single cell sequencing runs and by eliminating barriers to the incorporation of single sequencing into their NIH funded studies. The Chromium Connect will be located, administered, and maintained in the new Single Cell Processing Shared Resource on the third floor of the Moores Cancer Center – a shared resource that will offer automated single cell sequencing, single cell sorting for live cell assays, and digital spatial profiling - all technologies that are needed for advanced cancer research. The Chromium Connect will participate in the MCC cost recovery system to ensure partial recovery of funds for salaries, maintenance agreements, and supplies.

项目概要/摘要 需要资金购买 10X Genomics Chromium Connect 以满足当前和未来的需求 摩尔斯癌症中心 (MCC) 和医学院众多研究人员的需求 加州大学圣地亚哥分校。所要求的 10X Genomics Chromium Connect 是一款独一无二的机器人 通过集成单细胞分区、条形码和自动化单细胞测序工作流程的平台 将 RNA 文库制备整合为一种优化的解决方案，可缩短工作流程时间并降低门槛 用于进行单细胞分选和测序。单细胞测序正在迅速成为一种强大的工具 在基础、转化和临床癌症研究以及其他临床疾病的研究中。这 使用单细胞技术获得的知识深度正在改变新疗法的速度 针对癌症和其他疾病的治疗可以进行开发和微调。 10X 基因组学 Chromium Connect 平台简化单细胞测序工作流程，降低单细胞测序门槛 为所有用户进行分类和排序，尤其是在可以随时联系两个领域的专家的情况下 单细胞实验的设计和生成的测序数据的分析。该装置减少了 样品间的变异性并避免人为移液错误，从而增加了可靠性 对每次运行的数据收集进行排序。 Chromium Connect 的增强功能 圣地亚哥现有的仪器将为单细胞测序提供更多机会 向 MCC 用户提供技术，从而促进癌症预防、诊断和护理。 我们 NIH 资助的用户研究了各种项目，包括肿瘤微环境分析、 肿瘤免疫学、细胞信号传导、癌症干细胞生物学、癌症治疗和癌症护理， 代表了癌症和癌症治疗研究的多种方法。这些项目跨越 基础、转化和临床癌症研究。此外，我们的一些 MCC 调查员还进行 疾病生物学其他重要领域的研究。所有这些的共同点是高吞吐量、稳健的单 细胞测序工作流程将通过实现更可靠的单细胞测序运行来增强他们的研究 通过消除将单一测序纳入 NIH 资助的研究的障碍。 Chromium Connect 将在新的单细胞处理中进行定位、管理和维护 摩尔斯癌症中心三楼的共享资源——该共享资源将提供自动化服务 单细胞测序、活细胞分析的单细胞分选以及数字空间分析——所有技术 是高级癌症研究所需要的。 Chromium Connect 将参与 MCC 成本回收 确保部分回收工资、维护协议和用品资金的系统。