10X Chromium Connect for Single Cell Library Preparations for Translational Research

项目摘要/摘要 需要资金购买10倍基因组Chromium Connect以满足当前和未来的需求 摩尔癌症中心(MCC)和纽约大学医学院的众多研究人员的要求 加州大学圣地亚哥分校。所要求的10倍基因组学Chromium Connect是一款独一无二的机器人 通过集成单细胞划分、条形码和 将RNA文库准备整合到一个优化的解决方案中，从而缩短工作流程时间并降低门槛 用于执行单细胞分选和测序。单细胞测序正在迅速成为一种强大的工具 在基础、翻译和临床癌症研究以及其他临床疾病的研究中。这个 使用单细胞技术获得的知识深度正在改变新疗法的速度 对于癌症和其他疾病，可以开发和微调。10倍基因组学Chromium Connect 平台简化了单细胞测序工作流程，降低了执行单细胞测序的门槛 对所有用户进行分类和排序，特别是在与 设计单细胞实验和分析产生的测序数据。这个装置减少了 样品之间的可变性，避免了人为移液错误，从而增加了相当大的可靠性 从一次运行到另一次运行的排序数据收集。Chromium Connect Over的增强功能 圣地亚哥目前可用的仪器将为单细胞测序提供更多途径 向MCC用户提供技术，从而将促进癌症的预防、诊断和护理。 我们NIH资助的用户研究各种项目，从肿瘤微环境分析， 肿瘤免疫学、细胞信号转导、癌症干细胞生物学、癌症治疗和癌症护理， 代表了研究癌症和癌症治疗的各种方法。这些项目跨越了 基础、转化性和临床癌症研究。此外，我们的一些MCC调查人员进行了 疾病生物学其他重要领域的研究。它们都有一个共同点，那就是高吞吐量、健壮的单一 细胞测序工作流程将通过支持更可靠的单细胞测序运行和 通过消除将单一测序纳入NIH资助的研究的障碍。 Chromium Connect将在新的Single Cell Process中进行定位、管理和维护 摩尔斯癌症中心三楼的共享资源-一个共享资源，将提供自动化 单细胞测序、活细胞分析的单细胞分选和数字空间分析-所有这些技术都可以 是先进的癌症研究所需要的。Chromium Connect将参与MCC成本回收 确保部分收回工资、维修协议和用品资金的制度。

PROJECT SUMMARY/ABSTRACT Funds are requested to purchase a 10X Genomics Chromium Connect to meet the ongoing and future demands of numerous researchers within the Moores Cancer Center (MCC) and the School of Medicine at the University of California, San Diego. The requested 10X Genomics Chromium Connect is a one-of-a-kind robotic platform that automates single cell sequencing workflows by integrating single cell partitioning, barcoding, and RNA library preparation together into one optimized solution that reduces workflow time and lowers the threshold for performing single cell sorting and sequencing. Single cell sequencing is rapidly becoming a powerful tool in basic, translational, and clinical cancer research, as well as in the study of other clinical disorders. The depth of knowledge gained using single cell technologies is transforming the speed at which new therapies for cancer and other diseases can be developed and fine-tuned. The 10X Genomics Chromium Connect platform simplifies single cell sequencing workflows and lowers the threshold for performing single cell sorting and sequencing for all users, especially when coupled with ready access to experts in both the design of single cell experiments and the analysis of generated sequencing data. This device reduces sample to sample variability and avoids human pipetting errors, thus adding substantial reliability to sequencing data collection from run to run. The enhanced capabilities of the Chromium Connect over current instrumentation available in San Diego will provide greater access to single cell sequencing technologies to MCC users and thereby will advance cancer prevention, diagnosis, and care. Our NIH funded users study a variety of projects, ranging from analysis of the tumor microenvironment, tumor immunology, cell signaling, cancer stem cell biology, cancer therapeutics, and cancer care, representing a wide variety of approaches to the study of cancer and cancer therapy. These projects span basic, translational, and clinical cancer research. In addition, some of our MCC investigators conduct research in other significant areas of disease biology. All have in common that high throughput, robust single cell sequencing workflows will enhance their research by enabling more reliable single cell sequencing runs and by eliminating barriers to the incorporation of single sequencing into their NIH funded studies. The Chromium Connect will be located, administered, and maintained in the new Single Cell Processing Shared Resource on the third floor of the Moores Cancer Center – a shared resource that will offer automated single cell sequencing, single cell sorting for live cell assays, and digital spatial profiling - all technologies that are needed for advanced cancer research. The Chromium Connect will participate in the MCC cost recovery system to ensure partial recovery of funds for salaries, maintenance agreements, and supplies.