Impact of mucin fermenting microbes on the virulence of Pseudomonas aeruginosa in chronic rhinosinusitis
Impact of mucin fermenting microbes on the virulence of Pseudomonas aeruginosa in chronic rhinosinusitis
批准号:
10430744
负责人:
Do-Yeon Cho
金额:
$22.28万
依托单位国家:
美国
项目类别:
财政年份:
2022
资助国家:
美国
项目状态:
已结题
起止时间:
2022-04-19 至 2024-03-31
关键词:
AdultAffectAirway DiseaseAmericanAnaerobic BacteriaAntibiotic ResistanceAntibioticsAsthmaBacterial GenesBacteroides fragilisBenignBrain AbscessCT2584 HMSCarbonCellulitisChronic BronchitisChronic DiseaseChronic Obstructive Pulmonary DiseaseClinicalCongestive Heart FailureCystic FibrosisDNA ProbesDataDigoxinDiseaseEnvironmentEnzymesEpithelialEpoxide hydrolaseExhibitsEye InfectionsFermentationGene ExpressionGene ProteinsGram-Negative BacteriaGrowthHealthHealthcareHumanImpairmentIn Situ HybridizationIn VitroIndividualInfectionInflammationInflammatoryInvadedIrrigationIsocitrate LyaseLabelLeadLightLiquid substanceMalignant - descriptorMeasurementMethodsMicrobeModelingMucinsMucous MembraneNational Institute of Allergy and Infectious DiseaseNutrientOperative Surgical ProceduresOryctolagus cuniculusOtitis ExternaOutcomePathogenicityPatientsPeptic UlcerPhenotypePopulationProliferatingPseudomonas aeruginosaPseudomonas aeruginosa infectionPyruvate synthaseQuality of lifeResearchResearch PersonnelResolutionRibosomal RNARoleSalineSinusSourceSteroid therapyStreptococcusSubmucosaSymptomsSystemTechniquesTestingTissuesTranslatingVirulenceVirulence Factorsbone erosionchronic respiratory diseasechronic rhinosinusitisdesigneffective therapyexperimental studyhuman tissuein vivomacrophagemicrobialmicrobial communitymicrobiotamutantnovelpathogenprotein biomarkersprotein expressionresponsetranscriptome sequencingtreatment strategy
中文摘要
题目:黏素发酵菌对慢性铜绿假单胞菌毒力的影响
英文摘要
Title: Impact of mucin fermenting microbes on the virulence of Pseudomonas aeruginosa in chronic
rhinosinusitis
Project Summary/Abstract
Chronic rhinosinusitis (CRS) is a chronic airway disease defined as persistent inflammation and infection of the
nasal and sinus mucosa. Microbes affect human health through interspecies interactions, with a broad
spectrum of outcomes - some beneficial, others pathogenic. Disruption of the stable microbiota in the sinuses
and pathogen overgrowth can lead to benign microbial communities becoming pro-inflammatory and invasive.
Pseudomonas aeruginosa is an opportunistic, gram-negative bacterial species associated with severe,
recalcitrant CRS; CRS patients with P. aeruginosa correlate with poor symptom resolution and worse clinical
outcomes, leading to complications, such as orbital cellulitis and brain abscess, even in non-
immunocompromised individuals. Thus, identifying the mechanisms underlying the virulence of P. aeruginosa
in CRS progression is critical to developing new treatment strategies. Our aims are designed to test a
hypothesis that mucin-fermenting microbes promote the microbial virulence of P. aeruginosa, leading to the
progression of recalcitrant CRS.
(1) To evaluate whether enhanced P. aeruginosa virulence and CRS progression in vivo rely on mucin
fermentation by B. fragilis. We expect CRS rabbits inoculated with wild-type P. aeruginosa and wild-type B.
fragilis to exhibit the most severe CRS phenotype with marked tissue invasion and virulence-associated
gene/protein markers.
(2) To determine how mucin-fermenting microbes modulate P. aeruginosa tissue invasion and
antibiotic resistance in vitro. We expect to identify the critical actors in modulating antibiotic resistance and
invasion in human tissue.
We expect this proposal to answer fundamental questions regarding P. aeruginosa virulence in recalcitrant,
destructive CRS, which, in turn, can lead to develop new, effective treatment strategies for a disease that
exacts an enormous healthcare burden. The proposed experiments will also shed light on how interactions with
mucin-fermenting microbes influence other airway diseases associated with P. aeruginosa, such as malignant
otitis externa, cystic fibrosis, and chronic obstructive pulmonary disease.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Impact of mucin fermenting microbes on the virulence of Pseudomonas aeruginosa in chronic rhinosinusitis
-
批准号:10611454
-
项目类别:
-
资助金额:$18.56万
-
财政年份:2022
-
负责人:Do-Yeon Cho
-
依托单位:
Dysbiosis of the Sinus Microbiota in Chronic Rhinosinusitis
-
批准号:10165486
-
项目类别:
-
资助金额:$15.88万
-
财政年份:2019
-
负责人:Do-Yeon Cho
-
依托单位:
Dysbiosis of the Sinus Microbiota in Chronic Rhinosinusitis
-
批准号:9805155
-
项目类别:
-
资助金额:$15.4万
-
财政年份:2019
-
负责人:Do-Yeon Cho
-
依托单位:
海外基金