Core C: Epigenetics and Quantitative Proteomics

表观基因组学和定量蛋白质组学核心将为该计划提供高端组学分析 帮助识别和量化组蛋白表达和翻译后修饰(PTM)的项目 使用质谱学，并提供各种全基因组实验的信息学分析。专门知识 在基因组学方面的核心包括分析下一代测序产生的数据和单 细胞测序技术，绘制各种细胞类型和细胞状态的表观基因组图，包括老化的细胞 或处于衰老状态的细胞，探讨组蛋白PTMS与转录因子结合的关系 从功能上整合基因组、表观基因组和转录组。在蛋白质组学方面，核心是好的 有提供单一和组合组蛋白PTM分析的经验，跟踪细胞信号事件 (磷酸蛋白质组学)，对中心碳代谢相关分子进行靶向代谢组学，并 蛋白质-蛋白质相互作用的鉴定。这将包括进行无标记和稳定的同位素标记 在高分辨率Orbitrap质谱仪上进行蛋白质组和代谢组学实验，与Customer配对 数据分析和生物信息学。所有实验的结果将分发给所有计划项目团队 成员以促进进一步的互动，并为未来的实验得出有用的建议。核心由以下人员领导 本杰明·加西亚博士和兰叶敏博士共同带来了多年的免费专业知识、创新和 对P01项目的体验。

The Epigenomics and Quantitative Proteomics Core will provide high-end omics analyses for the Program Project to help identify and quantify protein expression and post-translational modifications (PTMs) on histone using mass spectrometry, and to provide informatics analyses from various genome-wide experiments. Expertise in the Core on the genomics side include analyzing data generated from next-generation sequencing and single- cell sequencing technologies, mapping the epigenome of various cell types and cell states including aging cells or cells in senescence, inquiring into the relationship of histone PTMs and transcription factor binding, and functionally integrating the genome, epigenome and transcriptome. On the proteomics side, the Core is well experienced to provide analysis of single and combinatorial histone PTMs, track cellular signaling events (phosphoproteomics), perform targeted metabolomics on central carbon metabolism related molecules and for identification of protein-protein interactions. This will include performing label-free and stable isotope labeled proteomics and metabolomics experiments on high-resolution Orbitrap mass spectrometers, paired with custom data analysis and bioinformatics. Results from all experiments will be disseminated to all Program Project team members to facilitate further interactions and elicit helpful suggestions for future experiments. The Core is led by Dr. Benjamin Garcia and Dr. Yemin Lan who together bring years of complimentary expertise, innovation and experience to the P01 project.